Details
| Translated title of the contribution | Detection of the potato tuber necrotic ringspot strain of potato virus Y (PVY(NTN)) by reverse transcription and immunocapture polymerase chain reaction |
|---|---|
| Original language | German |
| Pages (from-to) | 337-345 |
| Number of pages | 9 |
| Journal | Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz |
| Volume | 103 |
| Issue number | 4 |
| Publication status | Published - 1996 |
Abstract
The potato tuber necrotic ringspot strain of potato virus Y (PVY(NTN)) was distinguished from the other members of the PVY(N) strain group by RT- immunocapture polymerase chain reaction (RT-PRC). The obligonucleotid primers were placed in the 5'-non coding region and in the adjacent P1-protein gene of the PVY(NTN) genome. Both primers define a target sequence of 835 bp. Twelve virus isolates obtained from samples with tuber symptoms, together with the Hungarian isolate as the standard, gave the expected band in the gel, but five virus isolates of the PVY(O) group, 11 isolates of the PVY(N) group and one isolate of the PVY(C) group did not. Moreover, PVY(NTN) was detected in two potato samples without symptoms and in tomato samples. This indicates that PVY(NTN) infections do not lead necessarily to PTNRD symptoms and that tomatoes can act as a virus reservoir. Three California virus isolates which induced PTNRD symptoms did not lead to the expected 835 bp band in the gel, indicating that these isolates differ from those collected in Europe.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 103, No. 4, 1996, p. 337-345.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Nachwels des kartoffelknollen-ringnekrose-stammes des kartoffelvirus Y (PVY(NTN)) mit hilfe reverser transkription und immunocapture-polymerase- kettenreaktion
AU - Weidemann, H. L.
AU - Maiss, E.
PY - 1996
Y1 - 1996
N2 - The potato tuber necrotic ringspot strain of potato virus Y (PVY(NTN)) was distinguished from the other members of the PVY(N) strain group by RT- immunocapture polymerase chain reaction (RT-PRC). The obligonucleotid primers were placed in the 5'-non coding region and in the adjacent P1-protein gene of the PVY(NTN) genome. Both primers define a target sequence of 835 bp. Twelve virus isolates obtained from samples with tuber symptoms, together with the Hungarian isolate as the standard, gave the expected band in the gel, but five virus isolates of the PVY(O) group, 11 isolates of the PVY(N) group and one isolate of the PVY(C) group did not. Moreover, PVY(NTN) was detected in two potato samples without symptoms and in tomato samples. This indicates that PVY(NTN) infections do not lead necessarily to PTNRD symptoms and that tomatoes can act as a virus reservoir. Three California virus isolates which induced PTNRD symptoms did not lead to the expected 835 bp band in the gel, indicating that these isolates differ from those collected in Europe.
AB - The potato tuber necrotic ringspot strain of potato virus Y (PVY(NTN)) was distinguished from the other members of the PVY(N) strain group by RT- immunocapture polymerase chain reaction (RT-PRC). The obligonucleotid primers were placed in the 5'-non coding region and in the adjacent P1-protein gene of the PVY(NTN) genome. Both primers define a target sequence of 835 bp. Twelve virus isolates obtained from samples with tuber symptoms, together with the Hungarian isolate as the standard, gave the expected band in the gel, but five virus isolates of the PVY(O) group, 11 isolates of the PVY(N) group and one isolate of the PVY(C) group did not. Moreover, PVY(NTN) was detected in two potato samples without symptoms and in tomato samples. This indicates that PVY(NTN) infections do not lead necessarily to PTNRD symptoms and that tomatoes can act as a virus reservoir. Three California virus isolates which induced PTNRD symptoms did not lead to the expected 835 bp band in the gel, indicating that these isolates differ from those collected in Europe.
KW - latent infections
KW - monoclonal antibodies
KW - polyclonal antibodies
KW - PTNRD
KW - PVY strain groups
KW - tomatoes
UR - http://www.scopus.com/inward/record.url?scp=0029821575&partnerID=8YFLogxK
M3 - Artikel
AN - SCOPUS:0029821575
VL - 103
SP - 337
EP - 345
JO - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
JF - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
SN - 0340-8159
IS - 4
ER -