Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Dierk Fricke
  • Alexander Becker
  • Annice Heratizadeh
  • Sara Knigge
  • Lennart Jütte
  • Merve Wollweber
  • Thomas Werfel
  • Bernhard Wilhelm Roth
  • Birgit Glasmacher

External Research Organisations

  • NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development
  • Hannover Medical School (MHH)
  • Laser Zentrum Hannover e.V. (LZH)
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Details

Original languageEnglish
Article number1400
Number of pages16
JournalPolymers
Volume12
Issue number6
Publication statusPublished - 22 Jun 2020

Abstract

Electrospun polycaprolactone:gelatin (PCL:GT) fibre scaffolds are widely employed in the field of tissue implants. Here, the orientation of fibres plays an important role in regard to implantation due to the impact on the mechanical properties. Likewise, the orientation of collagen fibres in skin tissue is relevant for dermatology. State-of-the-art fibre orientation measurement methods like electron microscopy are time consuming and destructive. In this work, we demonstrate polarimetry as a non-invasive approach and evaluate its potential by measuring the Mueller matrix (MM) of gelatin and collagen containing samples as simple skin tissue phantoms. We demonstrate that it is possible to determine the orientation of PCL:GT fibre scaffolds within one MM measurement. Furthermore, we determine the structural orientation in collagen film samples. Currently, the diagnosis of skin diseases is often performed by image analysis or histopathology respectively, which are either subjective or invasive. The method presented, here, provides an interesting alternative approach for such investigations. Our findings indicate that the orientation of collagen fibres within skin lesions might be detectable by MM measurements in the future, which is of interest for skin diagnostics, and will be further investigated during the next step.

Keywords

    Collagen, Electrospinning, Fibre alignment, Gelatin, Mueller matrix, Raman spectroscopy, Tissue engineering

ASJC Scopus subject areas

Cite this

Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics. / Fricke, Dierk; Becker, Alexander; Heratizadeh, Annice et al.
In: Polymers, Vol. 12, No. 6, 1400, 22.06.2020.

Research output: Contribution to journalArticleResearchpeer review

Fricke, D, Becker, A, Heratizadeh, A, Knigge, S, Jütte, L, Wollweber, M, Werfel, T, Roth, BW & Glasmacher, B 2020, 'Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics', Polymers, vol. 12, no. 6, 1400. https://doi.org/10.3390/polym12061400
Fricke, D., Becker, A., Heratizadeh, A., Knigge, S., Jütte, L., Wollweber, M., Werfel, T., Roth, B. W., & Glasmacher, B. (2020). Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics. Polymers, 12(6), Article 1400. https://doi.org/10.3390/polym12061400
Fricke D, Becker A, Heratizadeh A, Knigge S, Jütte L, Wollweber M et al. Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics. Polymers. 2020 Jun 22;12(6):1400. doi: 10.3390/polym12061400
Fricke, Dierk ; Becker, Alexander ; Heratizadeh, Annice et al. / Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics. In: Polymers. 2020 ; Vol. 12, No. 6.
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abstract = "Electrospun polycaprolactone:gelatin (PCL:GT) fibre scaffolds are widely employed in the field of tissue implants. Here, the orientation of fibres plays an important role in regard to implantation due to the impact on the mechanical properties. Likewise, the orientation of collagen fibres in skin tissue is relevant for dermatology. State-of-the-art fibre orientation measurement methods like electron microscopy are time consuming and destructive. In this work, we demonstrate polarimetry as a non-invasive approach and evaluate its potential by measuring the Mueller matrix (MM) of gelatin and collagen containing samples as simple skin tissue phantoms. We demonstrate that it is possible to determine the orientation of PCL:GT fibre scaffolds within one MM measurement. Furthermore, we determine the structural orientation in collagen film samples. Currently, the diagnosis of skin diseases is often performed by image analysis or histopathology respectively, which are either subjective or invasive. The method presented, here, provides an interesting alternative approach for such investigations. Our findings indicate that the orientation of collagen fibres within skin lesions might be detectable by MM measurements in the future, which is of interest for skin diagnostics, and will be further investigated during the next step.",
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