Details
Original language | English |
---|---|
Article number | 1400 |
Number of pages | 16 |
Journal | Polymers |
Volume | 12 |
Issue number | 6 |
Publication status | Published - 22 Jun 2020 |
Abstract
Electrospun polycaprolactone:gelatin (PCL:GT) fibre scaffolds are widely employed in the field of tissue implants. Here, the orientation of fibres plays an important role in regard to implantation due to the impact on the mechanical properties. Likewise, the orientation of collagen fibres in skin tissue is relevant for dermatology. State-of-the-art fibre orientation measurement methods like electron microscopy are time consuming and destructive. In this work, we demonstrate polarimetry as a non-invasive approach and evaluate its potential by measuring the Mueller matrix (MM) of gelatin and collagen containing samples as simple skin tissue phantoms. We demonstrate that it is possible to determine the orientation of PCL:GT fibre scaffolds within one MM measurement. Furthermore, we determine the structural orientation in collagen film samples. Currently, the diagnosis of skin diseases is often performed by image analysis or histopathology respectively, which are either subjective or invasive. The method presented, here, provides an interesting alternative approach for such investigations. Our findings indicate that the orientation of collagen fibres within skin lesions might be detectable by MM measurements in the future, which is of interest for skin diagnostics, and will be further investigated during the next step.
Keywords
- Collagen, Electrospinning, Fibre alignment, Gelatin, Mueller matrix, Raman spectroscopy, Tissue engineering
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Polymers and Plastics
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In: Polymers, Vol. 12, No. 6, 1400, 22.06.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics
AU - Fricke, Dierk
AU - Becker, Alexander
AU - Heratizadeh, Annice
AU - Knigge, Sara
AU - Jütte, Lennart
AU - Wollweber, Merve
AU - Werfel, Thomas
AU - Roth, Bernhard Wilhelm
AU - Glasmacher, Birgit
N1 - Funding Information: Funding: This project is funded by the Lower Saxony Ministry for Culture and Science (MWK) through the program Tailored Light and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). The research project is also supported by the DFG in the framework of the Research Unit 2180 ”Graded Implants for Tendon–Bone Junctions”.
PY - 2020/6/22
Y1 - 2020/6/22
N2 - Electrospun polycaprolactone:gelatin (PCL:GT) fibre scaffolds are widely employed in the field of tissue implants. Here, the orientation of fibres plays an important role in regard to implantation due to the impact on the mechanical properties. Likewise, the orientation of collagen fibres in skin tissue is relevant for dermatology. State-of-the-art fibre orientation measurement methods like electron microscopy are time consuming and destructive. In this work, we demonstrate polarimetry as a non-invasive approach and evaluate its potential by measuring the Mueller matrix (MM) of gelatin and collagen containing samples as simple skin tissue phantoms. We demonstrate that it is possible to determine the orientation of PCL:GT fibre scaffolds within one MM measurement. Furthermore, we determine the structural orientation in collagen film samples. Currently, the diagnosis of skin diseases is often performed by image analysis or histopathology respectively, which are either subjective or invasive. The method presented, here, provides an interesting alternative approach for such investigations. Our findings indicate that the orientation of collagen fibres within skin lesions might be detectable by MM measurements in the future, which is of interest for skin diagnostics, and will be further investigated during the next step.
AB - Electrospun polycaprolactone:gelatin (PCL:GT) fibre scaffolds are widely employed in the field of tissue implants. Here, the orientation of fibres plays an important role in regard to implantation due to the impact on the mechanical properties. Likewise, the orientation of collagen fibres in skin tissue is relevant for dermatology. State-of-the-art fibre orientation measurement methods like electron microscopy are time consuming and destructive. In this work, we demonstrate polarimetry as a non-invasive approach and evaluate its potential by measuring the Mueller matrix (MM) of gelatin and collagen containing samples as simple skin tissue phantoms. We demonstrate that it is possible to determine the orientation of PCL:GT fibre scaffolds within one MM measurement. Furthermore, we determine the structural orientation in collagen film samples. Currently, the diagnosis of skin diseases is often performed by image analysis or histopathology respectively, which are either subjective or invasive. The method presented, here, provides an interesting alternative approach for such investigations. Our findings indicate that the orientation of collagen fibres within skin lesions might be detectable by MM measurements in the future, which is of interest for skin diagnostics, and will be further investigated during the next step.
KW - Collagen
KW - Electrospinning
KW - Fibre alignment
KW - Gelatin
KW - Mueller matrix
KW - Raman spectroscopy
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85087811625&partnerID=8YFLogxK
U2 - 10.3390/polym12061400
DO - 10.3390/polym12061400
M3 - Article
C2 - 32580462
AN - SCOPUS:85087811625
VL - 12
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 6
M1 - 1400
ER -