Details
| Original language | English |
|---|---|
| Pages (from-to) | 356-362 |
| Number of pages | 7 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 105 |
| Issue number | 2 |
| Early online date | 29 Sept 2016 |
| Publication status | Published - 1 Feb 2017 |
Abstract
The application of nanotechnology in biomedical field has enormous potential in basic and applied research. Micro or nanofibers produced by electrospinning technique offer excellent properties because of large specific surface area, high porosity, and ability to incorporate functional additives. Here we embedded biotinylated bovine serum albumin into polylactic acid (PLA)–polyethylene glycol (PEG) fibers, which enabled specific immobilization of fluorescently labelled avidin. An alkaline phosphatase enzyme was immobilized via biotin-streptavidin interaction on the hybrid nanofibers, demonstrating the suitability of the material for biosensing applications. These functional nanofibers provide a promising platform for development of biosensors and other biofunctional materials utilizing avidin–biotin as a generic and robust immobilization method.
Keywords
- avidin, biotinylated BSA, electrospinning, functional fibers, polyethylene glycol (PEG), polylactic acid (PLA)
ASJC Scopus subject areas
- Materials Science(all)
- Ceramics and Composites
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Materials Science(all)
- Metals and Alloys
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In: Journal of Biomedical Materials Research - Part A, Vol. 105, No. 2, 01.02.2017, p. 356-362.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mixture of PLA–PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers
AU - Kumar, Manish
AU - Rahikainen, Rolle
AU - Unruh, Daniel
AU - Hytönen, Vesa P.
AU - Delbrück, Cesare
AU - Sindelar, Ralf
AU - Renz, Franz
N1 - Publisher Copyright: © 2016 Wiley Periodicals, Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The application of nanotechnology in biomedical field has enormous potential in basic and applied research. Micro or nanofibers produced by electrospinning technique offer excellent properties because of large specific surface area, high porosity, and ability to incorporate functional additives. Here we embedded biotinylated bovine serum albumin into polylactic acid (PLA)–polyethylene glycol (PEG) fibers, which enabled specific immobilization of fluorescently labelled avidin. An alkaline phosphatase enzyme was immobilized via biotin-streptavidin interaction on the hybrid nanofibers, demonstrating the suitability of the material for biosensing applications. These functional nanofibers provide a promising platform for development of biosensors and other biofunctional materials utilizing avidin–biotin as a generic and robust immobilization method.
AB - The application of nanotechnology in biomedical field has enormous potential in basic and applied research. Micro or nanofibers produced by electrospinning technique offer excellent properties because of large specific surface area, high porosity, and ability to incorporate functional additives. Here we embedded biotinylated bovine serum albumin into polylactic acid (PLA)–polyethylene glycol (PEG) fibers, which enabled specific immobilization of fluorescently labelled avidin. An alkaline phosphatase enzyme was immobilized via biotin-streptavidin interaction on the hybrid nanofibers, demonstrating the suitability of the material for biosensing applications. These functional nanofibers provide a promising platform for development of biosensors and other biofunctional materials utilizing avidin–biotin as a generic and robust immobilization method.
KW - avidin
KW - biotinylated BSA
KW - electrospinning
KW - functional fibers
KW - polyethylene glycol (PEG)
KW - polylactic acid (PLA)
UR - http://www.scopus.com/inward/record.url?scp=84992410676&partnerID=8YFLogxK
U2 - https://trepo.tuni.fi/bitstream/10024/118107/2/Mixture_of_PLA-PEG_2017.pdf
DO - https://trepo.tuni.fi/bitstream/10024/118107/2/Mixture_of_PLA-PEG_2017.pdf
M3 - Article
C2 - 27684712
AN - SCOPUS:84992410676
VL - 105
SP - 356
EP - 362
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -