Details
| Original language | English |
|---|---|
| Article number | fiad047 |
| Journal | FEMS microbiology ecology |
| Volume | 99 |
| Issue number | 6 |
| Early online date | 8 May 2023 |
| Publication status | Published - Jun 2023 |
Abstract
A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.
Keywords
- landfill cover, methane, methylobacter, methylocystis, methylosarcina, pmoA, viral shunt
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Environmental Science(all)
- Ecology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: FEMS microbiology ecology, Vol. 99, No. 6, fiad047, 06.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mitomycin C-induced effects on aerobic methanotrophs in a landfill cover soil
T2 - implications of a viral shunt?
AU - Heffner, Tanja
AU - Kaupper, Thomas
AU - Heinrichs, Mara
AU - Lee, Hyo Jung
AU - Rüppel, Nadine
AU - Horn, Marcus A
AU - Ho, Adrian
N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (HO6234/1-2), and the Leibniz Universität Hannover, Germany.
PY - 2023/6
Y1 - 2023/6
N2 - A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.
AB - A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.
KW - landfill cover
KW - methane
KW - methylobacter
KW - methylocystis
KW - methylosarcina
KW - pmoA
KW - viral shunt
UR - http://www.scopus.com/inward/record.url?scp=85163087409&partnerID=8YFLogxK
U2 - 10.1093/femsec/fiad047
DO - 10.1093/femsec/fiad047
M3 - Article
C2 - 37156510
VL - 99
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
SN - 0168-6496
IS - 6
M1 - fiad047
ER -