TY - JOUR

T1 - Metal Nanoparticles in Laser Bioprinting

AU - Zhigarkov, Vyacheslav

AU - Volchkov, Ivan

AU - Yusupov, Vladimir

AU - Chichkov, Boris

N1 - Funding Information: This work was supported by the grant from the Russian Science Foundation 20?14-00286 in terms of conducting experiments, analyzing the obtained results, and improving bioprinting and LEMS technologies and partially by the Ministry of Science and Higher Education within the framework of the State Assignment of the Federal Research Center ?Crystallography and Photonics? of the Russian Academy of Sciences, in terms of using the equipment of the Center for Collective Use by providing SEM and AFM facilities.

PY - 2021/9/30

Y1 - 2021/9/30

N2 - Laser bioprinting is a promising method for applications in biotechnology, tissue engineering, and regenerative medicine. It is based on a microdroplet transfer from a donor slide induced by laser pulse heating of a thin metal absorption film covered with a layer of hydrogel containing living cells (bioink). Due to the presence of the metal absorption layer, some debris in the form of metal nanoparticles is printed together with bioink microdroplets. In this article, experimental investigations of the amount of metal nanoparticles formed during the laser bioprinting process and transported in bioink microdroplets are performed. As metal absorption layers, Ti films with the thickness in the range of 25–400 nm, produced by magnetron spattering, were applied. Dependences of the volume of bioink microdroplets and the amount of Ti nanoparticles within them on the laser pulse fluence were obtained. It has been experimentally found that practically all nanoparticles remain in the hydrogel layer on the donor slide during bioprinting, with only a small fraction of them transferred within the microdroplet (0.5% to 2.5%). These results are very important for applications of laser bioprinting since the transferred metal nanoparticles can potentially affect living systems. The good news is that the amount of such nanoparticles is very low to produce any negative effect on the printed cells.

AB - Laser bioprinting is a promising method for applications in biotechnology, tissue engineering, and regenerative medicine. It is based on a microdroplet transfer from a donor slide induced by laser pulse heating of a thin metal absorption film covered with a layer of hydrogel containing living cells (bioink). Due to the presence of the metal absorption layer, some debris in the form of metal nanoparticles is printed together with bioink microdroplets. In this article, experimental investigations of the amount of metal nanoparticles formed during the laser bioprinting process and transported in bioink microdroplets are performed. As metal absorption layers, Ti films with the thickness in the range of 25–400 nm, produced by magnetron spattering, were applied. Dependences of the volume of bioink microdroplets and the amount of Ti nanoparticles within them on the laser pulse fluence were obtained. It has been experimentally found that practically all nanoparticles remain in the hydrogel layer on the donor slide during bioprinting, with only a small fraction of them transferred within the microdroplet (0.5% to 2.5%). These results are very important for applications of laser bioprinting since the transferred metal nanoparticles can potentially affect living systems. The good news is that the amount of such nanoparticles is very low to produce any negative effect on the printed cells.

KW - Gel microdroplets

KW - Laser bioprinting

KW - Ti nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85116054323&partnerID=8YFLogxK

U2 - 10.3390/nano11102584

DO - 10.3390/nano11102584

M3 - Article

AN - SCOPUS:85116054323

VL - 11

JO - Nanomaterials

JF - Nanomaterials

SN - 2079-4991

IS - 10

M1 - 2584

ER -