Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Natalia Trufanova
  • Oleksandra Hubenia
  • Yurii Kot
  • Oleh Trufanov
  • Ihor Kovalenko
  • Kateryna Kot
  • Oleksandr Petrenko

Research Organisations

External Research Organisations

  • Institute for Problems of Cryobiology and Cryomedicine
  • Kharkov National University
View graph of relations

Details

Original languageEnglish
JournalBiopreservation and biobanking
Publication statusE-pub ahead of print - 26 Dec 2024

Abstract

Introduction: Human mesenchymal stromal cells (MSCs) are attractive for both medical practice and biomedical research. Nonfreezing short-term storage may provide safe and simple transportation and promote the practical use of MSCs. Objectives: We aimed to determine the duration of efficient storage at ambient temperature (22°C) of human dermal MSCs in different three-dimensional organization and to investigate the role of cell metabolic mode in the resistance to the ambient storage damaging factors. Methods: MSCs in monolayer, suspension, and encapsulated in alginate microspheres (AMS) were stored in sealed containers at 22°С in culture medium. Viability (fluorescein diacetate /ethidium bromide) and metabolic activity (Alamar Blue assay) were assessed at 0, 3, 7, 10, and 14 days of the storage. Mitochondrial membrane potential (JC-1 test), cell cycle analysis, reactive oxygen species level, and resistance to hydrogen peroxide were analyzed under culture conditions. Results: Alginate encapsulation was shown to maintain viability (about 85%), metabolic activity, and adhesion ability during storage for 7 days. The storage of MSCs in both monolayer and suspension was less efficient. Culture of MSCs in AMS decreased basal metabolic activity, mitochondrial activity, and led to reversible cell cycle arrest compared to standard two-dimensional culture. MSCs in AMS have a lower basal level of reactive oxygen species and higher resistance to hydrogen peroxide compared with those in monolayer culture. Conclusion: Revealed shift into quiescent metabolic mode is essential for alginate-encapsulated MSCs resistance to storage at ambient temperature.

Keywords

    alginate encapsulation, ambient temperature storage, cell cycle, mesenchymal stromal cells, metabolic activity, reactive oxygen species

ASJC Scopus subject areas

Cite this

Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature. / Trufanova, Natalia; Hubenia, Oleksandra; Kot, Yurii et al.
In: Biopreservation and biobanking, 26.12.2024.

Research output: Contribution to journalArticleResearchpeer review

Trufanova, N., Hubenia, O., Kot, Y., Trufanov, O., Kovalenko, I., Kot, K., & Petrenko, O. (2024). Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature. Biopreservation and biobanking. Advance online publication. https://doi.org/10.1089/bio.2024.0103
Trufanova N, Hubenia O, Kot Y, Trufanov O, Kovalenko I, Kot K et al. Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature. Biopreservation and biobanking. 2024 Dec 26. Epub 2024 Dec 26. doi: 10.1089/bio.2024.0103
Download
@article{4a981787b3e8474299bf552a844f6e58,
title = "Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature",
abstract = "Introduction: Human mesenchymal stromal cells (MSCs) are attractive for both medical practice and biomedical research. Nonfreezing short-term storage may provide safe and simple transportation and promote the practical use of MSCs. Objectives: We aimed to determine the duration of efficient storage at ambient temperature (22°C) of human dermal MSCs in different three-dimensional organization and to investigate the role of cell metabolic mode in the resistance to the ambient storage damaging factors. Methods: MSCs in monolayer, suspension, and encapsulated in alginate microspheres (AMS) were stored in sealed containers at 22°С in culture medium. Viability (fluorescein diacetate /ethidium bromide) and metabolic activity (Alamar Blue assay) were assessed at 0, 3, 7, 10, and 14 days of the storage. Mitochondrial membrane potential (JC-1 test), cell cycle analysis, reactive oxygen species level, and resistance to hydrogen peroxide were analyzed under culture conditions. Results: Alginate encapsulation was shown to maintain viability (about 85%), metabolic activity, and adhesion ability during storage for 7 days. The storage of MSCs in both monolayer and suspension was less efficient. Culture of MSCs in AMS decreased basal metabolic activity, mitochondrial activity, and led to reversible cell cycle arrest compared to standard two-dimensional culture. MSCs in AMS have a lower basal level of reactive oxygen species and higher resistance to hydrogen peroxide compared with those in monolayer culture. Conclusion: Revealed shift into quiescent metabolic mode is essential for alginate-encapsulated MSCs resistance to storage at ambient temperature.",
keywords = "alginate encapsulation, ambient temperature storage, cell cycle, mesenchymal stromal cells, metabolic activity, reactive oxygen species",
author = "Natalia Trufanova and Oleksandra Hubenia and Yurii Kot and Oleh Trufanov and Ihor Kovalenko and Kateryna Kot and Oleksandr Petrenko",
note = "Publisher Copyright: {\textcopyright} 2024, Mary Ann Liebert, Inc., publishers.",
year = "2024",
month = dec,
day = "26",
doi = "10.1089/bio.2024.0103",
language = "English",
journal = "Biopreservation and biobanking",
issn = "1947-5535",
publisher = "Mary Ann Liebert Inc.",

}

Download

TY - JOUR

T1 - Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature

AU - Trufanova, Natalia

AU - Hubenia, Oleksandra

AU - Kot, Yurii

AU - Trufanov, Oleh

AU - Kovalenko, Ihor

AU - Kot, Kateryna

AU - Petrenko, Oleksandr

N1 - Publisher Copyright: © 2024, Mary Ann Liebert, Inc., publishers.

PY - 2024/12/26

Y1 - 2024/12/26

N2 - Introduction: Human mesenchymal stromal cells (MSCs) are attractive for both medical practice and biomedical research. Nonfreezing short-term storage may provide safe and simple transportation and promote the practical use of MSCs. Objectives: We aimed to determine the duration of efficient storage at ambient temperature (22°C) of human dermal MSCs in different three-dimensional organization and to investigate the role of cell metabolic mode in the resistance to the ambient storage damaging factors. Methods: MSCs in monolayer, suspension, and encapsulated in alginate microspheres (AMS) were stored in sealed containers at 22°С in culture medium. Viability (fluorescein diacetate /ethidium bromide) and metabolic activity (Alamar Blue assay) were assessed at 0, 3, 7, 10, and 14 days of the storage. Mitochondrial membrane potential (JC-1 test), cell cycle analysis, reactive oxygen species level, and resistance to hydrogen peroxide were analyzed under culture conditions. Results: Alginate encapsulation was shown to maintain viability (about 85%), metabolic activity, and adhesion ability during storage for 7 days. The storage of MSCs in both monolayer and suspension was less efficient. Culture of MSCs in AMS decreased basal metabolic activity, mitochondrial activity, and led to reversible cell cycle arrest compared to standard two-dimensional culture. MSCs in AMS have a lower basal level of reactive oxygen species and higher resistance to hydrogen peroxide compared with those in monolayer culture. Conclusion: Revealed shift into quiescent metabolic mode is essential for alginate-encapsulated MSCs resistance to storage at ambient temperature.

AB - Introduction: Human mesenchymal stromal cells (MSCs) are attractive for both medical practice and biomedical research. Nonfreezing short-term storage may provide safe and simple transportation and promote the practical use of MSCs. Objectives: We aimed to determine the duration of efficient storage at ambient temperature (22°C) of human dermal MSCs in different three-dimensional organization and to investigate the role of cell metabolic mode in the resistance to the ambient storage damaging factors. Methods: MSCs in monolayer, suspension, and encapsulated in alginate microspheres (AMS) were stored in sealed containers at 22°С in culture medium. Viability (fluorescein diacetate /ethidium bromide) and metabolic activity (Alamar Blue assay) were assessed at 0, 3, 7, 10, and 14 days of the storage. Mitochondrial membrane potential (JC-1 test), cell cycle analysis, reactive oxygen species level, and resistance to hydrogen peroxide were analyzed under culture conditions. Results: Alginate encapsulation was shown to maintain viability (about 85%), metabolic activity, and adhesion ability during storage for 7 days. The storage of MSCs in both monolayer and suspension was less efficient. Culture of MSCs in AMS decreased basal metabolic activity, mitochondrial activity, and led to reversible cell cycle arrest compared to standard two-dimensional culture. MSCs in AMS have a lower basal level of reactive oxygen species and higher resistance to hydrogen peroxide compared with those in monolayer culture. Conclusion: Revealed shift into quiescent metabolic mode is essential for alginate-encapsulated MSCs resistance to storage at ambient temperature.

KW - alginate encapsulation

KW - ambient temperature storage

KW - cell cycle

KW - mesenchymal stromal cells

KW - metabolic activity

KW - reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=85213235729&partnerID=8YFLogxK

U2 - 10.1089/bio.2024.0103

DO - 10.1089/bio.2024.0103

M3 - Article

AN - SCOPUS:85213235729

JO - Biopreservation and biobanking

JF - Biopreservation and biobanking

SN - 1947-5535

ER -