Details
Original language | English |
---|---|
Article number | 100967 |
Journal | Current Research in Food Science |
Volume | 10 |
Early online date | 24 Dec 2024 |
Publication status | Published - 2025 |
Abstract
Chokeberry (Aronia melanocarpa) has been traditionally used as a folk remedy due to its health-promoting effects. The aim of this study was to investigate the effects of chokeberry polyphenols combined with the matrices of milk and milk alternatives on the permeability of the intestinal barrier. Based on this, in vitro availability of chokeberry polyphenols was tested by gastrointestinal model combined with a co-culture of human colon adenocarcinoma cells (Caco-2) and human colon cancer cells (HT29-MTX). Additionally, the antioxidant capacity of the samples was analyzed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. According to the results, both chokeberry juice and chokeberry juice in combination with milk showed a higher recovery of DPPH radical scavenging ability after intestinal digestion. Moreover, a significant difference in the transport of Lucifer Yellow through the intestinal membrane was observed when compared to the control. Therefore, fat- and protein-rich food matrices could represent a potential to increase the bioavailability of phenolic compounds while reducing intestinal barrier injury.
Keywords
- Antioxidant activity, Aronia, Berry, Bioaccessibility, Bioactive, Bioavailability, Cell culture, In vitro digestion, Milk, Polyphenol
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Sustainable Development Goals
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In: Current Research in Food Science, Vol. 10, 100967, 2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Metabolic fate of chokeberry (Aronia melanocarpa) phenolics in different food matrices
AU - Köpsel, Magdalena
AU - Ozkan, Gulay
AU - Esatbeyoglu, Tuba
N1 - Publisher Copyright: © 2024 The Authors
PY - 2025
Y1 - 2025
N2 - Chokeberry (Aronia melanocarpa) has been traditionally used as a folk remedy due to its health-promoting effects. The aim of this study was to investigate the effects of chokeberry polyphenols combined with the matrices of milk and milk alternatives on the permeability of the intestinal barrier. Based on this, in vitro availability of chokeberry polyphenols was tested by gastrointestinal model combined with a co-culture of human colon adenocarcinoma cells (Caco-2) and human colon cancer cells (HT29-MTX). Additionally, the antioxidant capacity of the samples was analyzed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. According to the results, both chokeberry juice and chokeberry juice in combination with milk showed a higher recovery of DPPH radical scavenging ability after intestinal digestion. Moreover, a significant difference in the transport of Lucifer Yellow through the intestinal membrane was observed when compared to the control. Therefore, fat- and protein-rich food matrices could represent a potential to increase the bioavailability of phenolic compounds while reducing intestinal barrier injury.
AB - Chokeberry (Aronia melanocarpa) has been traditionally used as a folk remedy due to its health-promoting effects. The aim of this study was to investigate the effects of chokeberry polyphenols combined with the matrices of milk and milk alternatives on the permeability of the intestinal barrier. Based on this, in vitro availability of chokeberry polyphenols was tested by gastrointestinal model combined with a co-culture of human colon adenocarcinoma cells (Caco-2) and human colon cancer cells (HT29-MTX). Additionally, the antioxidant capacity of the samples was analyzed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. According to the results, both chokeberry juice and chokeberry juice in combination with milk showed a higher recovery of DPPH radical scavenging ability after intestinal digestion. Moreover, a significant difference in the transport of Lucifer Yellow through the intestinal membrane was observed when compared to the control. Therefore, fat- and protein-rich food matrices could represent a potential to increase the bioavailability of phenolic compounds while reducing intestinal barrier injury.
KW - Antioxidant activity
KW - Aronia
KW - Berry
KW - Bioaccessibility
KW - Bioactive
KW - Bioavailability
KW - Cell culture
KW - In vitro digestion
KW - Milk
KW - Polyphenol
UR - http://www.scopus.com/inward/record.url?scp=85214574390&partnerID=8YFLogxK
U2 - 10.1016/j.crfs.2024.100967
DO - 10.1016/j.crfs.2024.100967
M3 - Article
VL - 10
JO - Current Research in Food Science
JF - Current Research in Food Science
M1 - 100967
ER -