Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Pravda Stoeva
  • Margarita Yankulova
  • Velichka Nikolaeva
  • Rositza Bachvarova
  • Lidia Ivanova
  • Edgar Maiss
  • Guenter Adam
  • Vladimir Vulkov
  • Spas Guelemerov
  • Atanas Atanassov

Research Organisations

External Research Organisations

  • De Montfort University
  • Institute of Genetic Engineering
  • Universität Hamburg
  • Tobacco Breeding Station
View graph of relations

Details

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalMolecular breeding
Volume4
Issue number2
Publication statusPublished - Apr 1998

Abstract

We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.

Keywords

    Field trials, Nucleoprotein gene-mediated resistance, Transgenic tobacco, TSWV

ASJC Scopus subject areas

Cite this

Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests. / Stoeva, Pravda; Yankulova, Margarita; Nikolaeva, Velichka et al.
In: Molecular breeding, Vol. 4, No. 2, 04.1998, p. 155-164.

Research output: Contribution to journalArticleResearchpeer review

Stoeva, P, Yankulova, M, Nikolaeva, V, Bachvarova, R, Ivanova, L, Maiss, E, Adam, G, Vulkov, V, Guelemerov, S & Atanassov, A 1998, 'Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests', Molecular breeding, vol. 4, no. 2, pp. 155-164. https://doi.org/10.1023/A:1009644315295
Stoeva, P., Yankulova, M., Nikolaeva, V., Bachvarova, R., Ivanova, L., Maiss, E., Adam, G., Vulkov, V., Guelemerov, S., & Atanassov, A. (1998). Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests. Molecular breeding, 4(2), 155-164. https://doi.org/10.1023/A:1009644315295
Stoeva P, Yankulova M, Nikolaeva V, Bachvarova R, Ivanova L, Maiss E et al. Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests. Molecular breeding. 1998 Apr;4(2):155-164. doi: 10.1023/A:1009644315295
Download
@article{544d831e67734a19b2c167b7072c49e7,
title = "Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: Greenhouse and field tests",
abstract = "We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.",
keywords = "Field trials, Nucleoprotein gene-mediated resistance, Transgenic tobacco, TSWV",
author = "Pravda Stoeva and Margarita Yankulova and Velichka Nikolaeva and Rositza Bachvarova and Lidia Ivanova and Edgar Maiss and Guenter Adam and Vladimir Vulkov and Spas Guelemerov and Atanas Atanassov",
year = "1998",
month = apr,
doi = "10.1023/A:1009644315295",
language = "English",
volume = "4",
pages = "155--164",
journal = "Molecular breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",
number = "2",

}

Download

TY - JOUR

T1 - Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene

T2 - Greenhouse and field tests

AU - Stoeva, Pravda

AU - Yankulova, Margarita

AU - Nikolaeva, Velichka

AU - Bachvarova, Rositza

AU - Ivanova, Lidia

AU - Maiss, Edgar

AU - Adam, Guenter

AU - Vulkov, Vladimir

AU - Guelemerov, Spas

AU - Atanassov, Atanas

PY - 1998/4

Y1 - 1998/4

N2 - We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.

AB - We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.

KW - Field trials

KW - Nucleoprotein gene-mediated resistance

KW - Transgenic tobacco

KW - TSWV

UR - http://www.scopus.com/inward/record.url?scp=7144227295&partnerID=8YFLogxK

U2 - 10.1023/A:1009644315295

DO - 10.1023/A:1009644315295

M3 - Article

AN - SCOPUS:7144227295

VL - 4

SP - 155

EP - 164

JO - Molecular breeding

JF - Molecular breeding

SN - 1380-3743

IS - 2

ER -