Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Susanne C Hammer
  • Annegret Becker
  • Katja Rateitschak
  • Annika Mohr
  • Florenza Lüder Ripoli
  • Silvia Hennecke
  • Johannes Junginger
  • Marion Hewicker-Trautwein
  • Bertram Brenig
  • Anaclet Ngezahayo
  • Ingo Nolte
  • Hugo Murua Escobar

External Research Organisations

  • University of Veterinary Medicine of Hannover, Foundation
  • University of Greifswald
  • University of Göttingen
  • Center for Systems Neuroscience Hannover (ZSN)
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Details

Original languageEnglish
Article number1655
JournalInternational Journal of Molecular Sciences
Volume17
Issue number10
Publication statusPublished - 29 Sept 2016

Abstract

Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.

Keywords

    Canine, Cell culture, Cell lines, Claudin, Mammary neoplasias, Marker expression

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines. / Hammer, Susanne C; Becker, Annegret; Rateitschak, Katja et al.
In: International Journal of Molecular Sciences, Vol. 17, No. 10, 1655, 29.09.2016.

Research output: Contribution to journalArticleResearchpeer review

Hammer, SC, Becker, A, Rateitschak, K, Mohr, A, Lüder Ripoli, F, Hennecke, S, Junginger, J, Hewicker-Trautwein, M, Brenig, B, Ngezahayo, A, Nolte, I & Murua Escobar, H 2016, 'Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines', International Journal of Molecular Sciences, vol. 17, no. 10, 1655. https://doi.org/10.3390/ijms17101655
Hammer, S. C., Becker, A., Rateitschak, K., Mohr, A., Lüder Ripoli, F., Hennecke, S., Junginger, J., Hewicker-Trautwein, M., Brenig, B., Ngezahayo, A., Nolte, I., & Murua Escobar, H. (2016). Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines. International Journal of Molecular Sciences, 17(10), Article 1655. https://doi.org/10.3390/ijms17101655
Hammer SC, Becker A, Rateitschak K, Mohr A, Lüder Ripoli F, Hennecke S et al. Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines. International Journal of Molecular Sciences. 2016 Sept 29;17(10):1655. doi: 10.3390/ijms17101655
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abstract = "Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.",
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AU - Hammer, Susanne C

AU - Becker, Annegret

AU - Rateitschak, Katja

AU - Mohr, Annika

AU - Lüder Ripoli, Florenza

AU - Hennecke, Silvia

AU - Junginger, Johannes

AU - Hewicker-Trautwein, Marion

AU - Brenig, Bertram

AU - Ngezahayo, Anaclet

AU - Nolte, Ingo

AU - Murua Escobar, Hugo

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Y1 - 2016/9/29

N2 - Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages the claudin expressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.

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