Details
Original language | English |
---|---|
Pages (from-to) | 474-481 |
Number of pages | 8 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 1808 |
Issue number | 1 |
Early online date | 29 Sept 2010 |
Publication status | Published - Jan 2011 |
Abstract
Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 °C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition.
Keywords
- Cholesterol transfer, Erythrocyte, Fourier transform infrared spectroscopy, Lipid phase behavior, Liposome, MALDI-TOF mass spectrometry
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1808, No. 1, 01.2011, p. 474-481.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Liposomes alter thermal phase behavior and composition of red blood cell membranes
AU - Stoll, Christoph
AU - Stadnick, Hart
AU - Kollas, Oliver
AU - Holovati, Jelena L.
AU - Glasmacher, Birgit
AU - Acker, Jason P.
AU - Wolkers, Willem F.
N1 - Funding Information: This work is supported by funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy). This study was partially funded from a grant “Liposomes in Transfusion Medicine: An Approach for Reducing the Red Blood Cell Hypothermic Storage Lesion” to JA from the Canadian Blood Services/Canadian Institutes for Health Research . We kindly acknowledge Dr. Heuft from the Hanover Medical school blood donation service for providing blood samples for the experiments. Furthermore, we acknowledge Dr. Ion Stoll from the University of Bielefeld, Department of Chemistry for ingenious theoretical cooperation in this project.
PY - 2011/1
Y1 - 2011/1
N2 - Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 °C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition.
AB - Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 °C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition.
KW - Cholesterol transfer
KW - Erythrocyte
KW - Fourier transform infrared spectroscopy
KW - Lipid phase behavior
KW - Liposome
KW - MALDI-TOF mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=78649779940&partnerID=8YFLogxK
U2 - 10.1016/j.bbamem.2010.09.012
DO - 10.1016/j.bbamem.2010.09.012
M3 - Article
C2 - 20883663
AN - SCOPUS:78649779940
VL - 1808
SP - 474
EP - 481
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -