Liposomes alter thermal phase behavior and composition of red blood cell membranes

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Christoph Stoll
  • Hart Stadnick
  • Oliver Kollas
  • Jelena L. Holovati
  • Birgit Glasmacher
  • Jason P. Acker
  • Willem F. Wolkers

Research Organisations

External Research Organisations

  • University of Alberta
  • Bielefeld University
  • Canadian Blood Services
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Details

Original languageEnglish
Pages (from-to)474-481
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1808
Issue number1
Early online date29 Sept 2010
Publication statusPublished - Jan 2011

Abstract

Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 °C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition.

Keywords

    Cholesterol transfer, Erythrocyte, Fourier transform infrared spectroscopy, Lipid phase behavior, Liposome, MALDI-TOF mass spectrometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biophysics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Liposomes alter thermal phase behavior and composition of red blood cell membranes. / Stoll, Christoph; Stadnick, Hart; Kollas, Oliver et al.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1808, No. 1, 01.2011, p. 474-481.

Research output: Contribution to journalArticleResearchpeer review

Stoll, C, Stadnick, H, Kollas, O, Holovati, JL, Glasmacher, B, Acker, JP & Wolkers, WF 2011, 'Liposomes alter thermal phase behavior and composition of red blood cell membranes', Biochimica et Biophysica Acta - Biomembranes, vol. 1808, no. 1, pp. 474-481. https://doi.org/10.1016/j.bbamem.2010.09.012
Stoll, C., Stadnick, H., Kollas, O., Holovati, J. L., Glasmacher, B., Acker, J. P., & Wolkers, W. F. (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta - Biomembranes, 1808(1), 474-481. https://doi.org/10.1016/j.bbamem.2010.09.012
Stoll C, Stadnick H, Kollas O, Holovati JL, Glasmacher B, Acker JP et al. Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochimica et Biophysica Acta - Biomembranes. 2011 Jan;1808(1):474-481. Epub 2010 Sept 29. doi: 10.1016/j.bbamem.2010.09.012
Stoll, Christoph ; Stadnick, Hart ; Kollas, Oliver et al. / Liposomes alter thermal phase behavior and composition of red blood cell membranes. In: Biochimica et Biophysica Acta - Biomembranes. 2011 ; Vol. 1808, No. 1. pp. 474-481.
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title = "Liposomes alter thermal phase behavior and composition of red blood cell membranes",
abstract = "Unilamellar liposomes composed of natural phospholipids provide a new promising class of protective agents for hypothermic storage, cryopreservation, or freeze-drying of red blood cells (RBCs). In this study, FTIR spectroscopy, MALDI-TOF MS, and colorimetric assays were used to investigate the effects of liposomes composed of a homologous series of linear saturated phosphatidylcholine phospholipids (18:0; 16:0; 14:0; 12:0) on RBC membranes. RBCs were incubated with liposomes at 37 °C and both the liposomal and the RBC fraction were analyzed after incubation. FTIR studies showed that liposomes composed of short acyl chain length lipids cause an increase in RBC membrane conformational disorder at suprazero temperatures, whereas long acyl chain length lipids were found to have little effects. The increased lipid conformational disorder in the RBC membranes coincided with a decrease in the cholesterol-to-phospholipid ratio. The opposite effects were found in the liposomes after incubation with RBCs. MALDI-TOF MS analysis showed the presence of short acyl chain length lipids (14:0 and 12:0) in RBC membranes after incubation, which was not observed after incubation with liposomes containing long acyl chain length lipids (18:0 and 16:0). Liposomes alter RBC membrane properties by cholesterol depletion and lipid addition.",
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T1 - Liposomes alter thermal phase behavior and composition of red blood cell membranes

AU - Stoll, Christoph

AU - Stadnick, Hart

AU - Kollas, Oliver

AU - Holovati, Jelena L.

AU - Glasmacher, Birgit

AU - Acker, Jason P.

AU - Wolkers, Willem F.

N1 - Funding Information: This work is supported by funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy). This study was partially funded from a grant “Liposomes in Transfusion Medicine: An Approach for Reducing the Red Blood Cell Hypothermic Storage Lesion” to JA from the Canadian Blood Services/Canadian Institutes for Health Research . We kindly acknowledge Dr. Heuft from the Hanover Medical school blood donation service for providing blood samples for the experiments. Furthermore, we acknowledge Dr. Ion Stoll from the University of Bielefeld, Department of Chemistry for ingenious theoretical cooperation in this project.

PY - 2011/1

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KW - Lipid phase behavior

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