Details
| Original language | English |
|---|---|
| Article number | 102330 |
| Journal | Current Opinion in Plant Biology |
| Volume | 71 |
| Early online date | 2 Jan 2023 |
| Publication status | Published - Feb 2023 |
Abstract
Major hurdles in plant biosynthetic pathway elucidation and engineering include the need for rapid testing of enzyme candidates and the lack of complex substrates that are often not accumulated in the plant, amenable to synthesis, or commercially available. Linking metabolic engineering with gene discovery in both yeast and plant holds great promise to expedite the elucidation process and, at the same time, provide a platform for the sustainable production of plant metabolites. In this review, we highlight how synthetic biology and metabolic engineering alleviated longstanding obstacles in plant pathway elucidation. Recent advances in developing these chassis that showcase established and emerging strategies in accelerating biosynthetic gene discovery will also be discussed.
Keywords
- Gene discovery, Metabolic engineering, Nicotiana benthamiana, Pathway elucidation, Plant specialized metabolism, Yeast
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Current Opinion in Plant Biology, Vol. 71, 102330, 02.2023.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Leveraging synthetic biology and metabolic engineering to overcome obstacles in plant pathway elucidation
AU - Kwan, Brooke D.
AU - Seligmann, Benedikt
AU - Nguyen, Trinh-Don
AU - Franke, Jakob
AU - Dang, Thu-Thuy T.
N1 - Funding Information: TTTD received funding from the Canada Natural Science and Engineering Research Council, NSERC Alliance International (ALLRP 571673 – 21), and the Michael Smith Foundation for Health Research Scholar (SCH- 2020-0401). JF received funding from the Bioeconomy International 2020 programme of the Federal Ministry of Education and Research (BMBF) Germany (grant 031B1208).
PY - 2023/2
Y1 - 2023/2
N2 - Major hurdles in plant biosynthetic pathway elucidation and engineering include the need for rapid testing of enzyme candidates and the lack of complex substrates that are often not accumulated in the plant, amenable to synthesis, or commercially available. Linking metabolic engineering with gene discovery in both yeast and plant holds great promise to expedite the elucidation process and, at the same time, provide a platform for the sustainable production of plant metabolites. In this review, we highlight how synthetic biology and metabolic engineering alleviated longstanding obstacles in plant pathway elucidation. Recent advances in developing these chassis that showcase established and emerging strategies in accelerating biosynthetic gene discovery will also be discussed.
AB - Major hurdles in plant biosynthetic pathway elucidation and engineering include the need for rapid testing of enzyme candidates and the lack of complex substrates that are often not accumulated in the plant, amenable to synthesis, or commercially available. Linking metabolic engineering with gene discovery in both yeast and plant holds great promise to expedite the elucidation process and, at the same time, provide a platform for the sustainable production of plant metabolites. In this review, we highlight how synthetic biology and metabolic engineering alleviated longstanding obstacles in plant pathway elucidation. Recent advances in developing these chassis that showcase established and emerging strategies in accelerating biosynthetic gene discovery will also be discussed.
KW - Gene discovery
KW - Metabolic engineering
KW - Nicotiana benthamiana
KW - Pathway elucidation
KW - Plant specialized metabolism
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=85145369488&partnerID=8YFLogxK
U2 - 10.1016/j.pbi.2022.102330
DO - 10.1016/j.pbi.2022.102330
M3 - Review article
AN - SCOPUS:85145369488
VL - 71
JO - Current Opinion in Plant Biology
JF - Current Opinion in Plant Biology
SN - 1369-5266
M1 - 102330
ER -