Details
| Original language | English |
|---|---|
| Article number | 1649419 |
| Journal | Frontiers in Oral Health |
| Volume | 6 |
| Publication status | Published - 4 Sept 2025 |
Abstract
Introduction: Changes in bacterial species composition within oral biofilms, known as biofilm dysbiosis, are associated with the development of severe oral diseases. To better understand this process and help establish early detection systems, models are needed which replicate oral biofilm dysbiosis in vitro – ideally by also mimicking natural salivary flow conditions. Methods: For this purpose, the present study cultivated two different combinations of oral commensal and pathogenic strains – Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar/parvula, Fusobacterium nucleatum and Porphyromonas gingivalis – comparatively within an established flow chamber model on the implant material titanium, and statically in 6-well plates for 21 days. Biofilm morphology, species distribution, and bacterial metabolism were analyzed by fluorescence microscopy, molecular biological methods, and metabolic interaction prediction. Results: Biofilm growth and composition were strongly influenced by bacterial species selection, and to a more minor extent, by cultivation conditions. Within the model containing V. dispar and a laboratory P. gingivalis strain, a diversification of commensal species was observed over time along with a significantly reduced pH-value. In contrast, the model containing V. parvula and the clinical isolate P. gingivalis W83, a dysbiotic shift with increased pathogen levels, pH-value, and virulence factors was achieved. Conclusion: Within the present study, different in vitro oral multispecies biofilm models were successfully developed. Depending on bacterial species selection, these models were able to depict the infection-associated dysbiotic shift in species composition under flow conditions solely by intrinsic interactions and without the use of external stimuli.
Keywords
- dental implants, dental plaque, dynamic cultivation, dysbiosis, microbiological techniques
ASJC Scopus subject areas
- Dentistry(all)
- Oral Surgery
- Dentistry(all)
- Dentistry (miscellaneous)
- Dentistry(all)
- Periodontics
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Frontiers in Oral Health, Vol. 6, 1649419, 04.09.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Influence of species composition and cultivation condition on peri-implant biofilm dysbiosis in vitro
AU - Heine, Nils
AU - Bittroff, Kristina
AU - Szafrański, Szymon P.
AU - Duitscher, Maya
AU - Behrens, Wiebke
AU - Vollmer, Clarissa
AU - Mikolai, Carina
AU - Kommerein, Nadine
AU - Debener, Nicolas
AU - Frings, Katharina
AU - Heisterkamp, Alexander
AU - Scheper, Thomas
AU - Torres-Mapa, Maria L.
AU - Bahnemann, Janina
AU - Stiesch, Meike
AU - Doll-Nikutta, Katharina
N1 - Publisher Copyright: 2025 Heine, Bittroff, Szafrański, Duitscher, Behrens, Vollmer, Mikolai, Kommerein, Debener, Frings, Heisterkamp, Scheper, Torres-Mapa, Bahnemann, Stiesch and Doll-Nikutta.
PY - 2025/9/4
Y1 - 2025/9/4
N2 - Introduction: Changes in bacterial species composition within oral biofilms, known as biofilm dysbiosis, are associated with the development of severe oral diseases. To better understand this process and help establish early detection systems, models are needed which replicate oral biofilm dysbiosis in vitro – ideally by also mimicking natural salivary flow conditions. Methods: For this purpose, the present study cultivated two different combinations of oral commensal and pathogenic strains – Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar/parvula, Fusobacterium nucleatum and Porphyromonas gingivalis – comparatively within an established flow chamber model on the implant material titanium, and statically in 6-well plates for 21 days. Biofilm morphology, species distribution, and bacterial metabolism were analyzed by fluorescence microscopy, molecular biological methods, and metabolic interaction prediction. Results: Biofilm growth and composition were strongly influenced by bacterial species selection, and to a more minor extent, by cultivation conditions. Within the model containing V. dispar and a laboratory P. gingivalis strain, a diversification of commensal species was observed over time along with a significantly reduced pH-value. In contrast, the model containing V. parvula and the clinical isolate P. gingivalis W83, a dysbiotic shift with increased pathogen levels, pH-value, and virulence factors was achieved. Conclusion: Within the present study, different in vitro oral multispecies biofilm models were successfully developed. Depending on bacterial species selection, these models were able to depict the infection-associated dysbiotic shift in species composition under flow conditions solely by intrinsic interactions and without the use of external stimuli.
AB - Introduction: Changes in bacterial species composition within oral biofilms, known as biofilm dysbiosis, are associated with the development of severe oral diseases. To better understand this process and help establish early detection systems, models are needed which replicate oral biofilm dysbiosis in vitro – ideally by also mimicking natural salivary flow conditions. Methods: For this purpose, the present study cultivated two different combinations of oral commensal and pathogenic strains – Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar/parvula, Fusobacterium nucleatum and Porphyromonas gingivalis – comparatively within an established flow chamber model on the implant material titanium, and statically in 6-well plates for 21 days. Biofilm morphology, species distribution, and bacterial metabolism were analyzed by fluorescence microscopy, molecular biological methods, and metabolic interaction prediction. Results: Biofilm growth and composition were strongly influenced by bacterial species selection, and to a more minor extent, by cultivation conditions. Within the model containing V. dispar and a laboratory P. gingivalis strain, a diversification of commensal species was observed over time along with a significantly reduced pH-value. In contrast, the model containing V. parvula and the clinical isolate P. gingivalis W83, a dysbiotic shift with increased pathogen levels, pH-value, and virulence factors was achieved. Conclusion: Within the present study, different in vitro oral multispecies biofilm models were successfully developed. Depending on bacterial species selection, these models were able to depict the infection-associated dysbiotic shift in species composition under flow conditions solely by intrinsic interactions and without the use of external stimuli.
KW - dental implants
KW - dental plaque
KW - dynamic cultivation
KW - dysbiosis
KW - microbiological techniques
UR - http://www.scopus.com/inward/record.url?scp=105016582488&partnerID=8YFLogxK
U2 - 10.3389/froh.2025.1649419
DO - 10.3389/froh.2025.1649419
M3 - Article
AN - SCOPUS:105016582488
VL - 6
JO - Frontiers in Oral Health
JF - Frontiers in Oral Health
M1 - 1649419
ER -