In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Tobias Ehmke
  • Janine Leckelt
  • Maria Reichard
  • Heike Weiss
  • Marina Hovakimyan
  • Alexander Heisterkamp
  • Oliver Stachs
  • Simone Baltrusch

Research Organisations

External Research Organisations

  • Laser Zentrum Hannover e.V. (LZH)
  • University of Rostock
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Details

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalExperimental Eye Research
Volume146
Publication statusPublished - 8 Dec 2015

Abstract

Two-photon microscopy (TPM) allows high contrast imaging at a subcellular resolution scale. In this work, the microscopy technique was applied to visualize corneal structures in two mouse models (BALB/c and B6.Cg-Tg(Thy1-YFP)16Jrs/J) in vivo. In particular, the transgenic Thy1-YFP mice expressing the yellow fluorescent protein (YFP) in all motor and sensory neurons had been used for investigating the nerve fiber density in healthy and streptozotocin-diabetic mice. This model is clinically relevant since patients suffering from diabetes mellitus have a high risk to develop small fiber neuropathy. Nonlinear laser scanning microscopy displayed a reduction of nerve fiber density in streptozotocin-diabetic versus healthy mice and confirmed data obtained by confocal laser scanning microscopy (CLSM). In recent years, corneal CLSM was proved to be an appropriate non-invasive tool for an early diagnosis of diabetic neuropathy. Nevertheless, validation of the CLSM method for the clinical routine is currently a matter of investigation and requires confirmation by further studies and complementary techniques. Thus, the present study provides further evidence of corneal confocal microscopy as a promising technique for non-invasive detection of diabetic neuropathy. Information derived from these experiments may become clinically relevant and help to develop new drugs for treatment of diabetic neuropathy.

Keywords

    Applied optics, Confocal microscopy, Cornea, Diabetic neuropathy, Nonlinear microscopy, Ophthalmology

ASJC Scopus subject areas

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Cite this

In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice. / Ehmke, Tobias; Leckelt, Janine; Reichard, Maria et al.
In: Experimental Eye Research, Vol. 146, 08.12.2015, p. 137-144.

Research output: Contribution to journalArticleResearchpeer review

Ehmke, T, Leckelt, J, Reichard, M, Weiss, H, Hovakimyan, M, Heisterkamp, A, Stachs, O & Baltrusch, S 2015, 'In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice', Experimental Eye Research, vol. 146, pp. 137-144. https://doi.org/10.1016/j.exer.2015.11.024
Ehmke, T., Leckelt, J., Reichard, M., Weiss, H., Hovakimyan, M., Heisterkamp, A., Stachs, O., & Baltrusch, S. (2015). In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice. Experimental Eye Research, 146, 137-144. https://doi.org/10.1016/j.exer.2015.11.024
Ehmke T, Leckelt J, Reichard M, Weiss H, Hovakimyan M, Heisterkamp A et al. In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice. Experimental Eye Research. 2015 Dec 8;146:137-144. doi: 10.1016/j.exer.2015.11.024
Ehmke, Tobias ; Leckelt, Janine ; Reichard, Maria et al. / In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice. In: Experimental Eye Research. 2015 ; Vol. 146. pp. 137-144.
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title = "In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice",
abstract = "Two-photon microscopy (TPM) allows high contrast imaging at a subcellular resolution scale. In this work, the microscopy technique was applied to visualize corneal structures in two mouse models (BALB/c and B6.Cg-Tg(Thy1-YFP)16Jrs/J) in vivo. In particular, the transgenic Thy1-YFP mice expressing the yellow fluorescent protein (YFP) in all motor and sensory neurons had been used for investigating the nerve fiber density in healthy and streptozotocin-diabetic mice. This model is clinically relevant since patients suffering from diabetes mellitus have a high risk to develop small fiber neuropathy. Nonlinear laser scanning microscopy displayed a reduction of nerve fiber density in streptozotocin-diabetic versus healthy mice and confirmed data obtained by confocal laser scanning microscopy (CLSM). In recent years, corneal CLSM was proved to be an appropriate non-invasive tool for an early diagnosis of diabetic neuropathy. Nevertheless, validation of the CLSM method for the clinical routine is currently a matter of investigation and requires confirmation by further studies and complementary techniques. Thus, the present study provides further evidence of corneal confocal microscopy as a promising technique for non-invasive detection of diabetic neuropathy. Information derived from these experiments may become clinically relevant and help to develop new drugs for treatment of diabetic neuropathy.",
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AU - Ehmke, Tobias

AU - Leckelt, Janine

AU - Reichard, Maria

AU - Weiss, Heike

AU - Hovakimyan, Marina

AU - Heisterkamp, Alexander

AU - Stachs, Oliver

AU - Baltrusch, Simone

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