Details
| Original language | English |
|---|---|
| Pages (from-to) | 1354-1359 |
| Number of pages | 6 |
| Journal | Acta biomaterialia |
| Volume | 10 |
| Issue number | 3 |
| Early online date | 19 Dec 2013 |
| Publication status | Published - Mar 2014 |
Abstract
Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37 C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25 C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
Keywords
- Drug delivery, Inclusion bodies, Nanomedicine, Protein release, Surface functionalization
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Materials Science(all)
- Biomaterials
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Engineering(all)
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Acta biomaterialia, Vol. 10, No. 3, 03.2014, p. 1354-1359.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates
AU - Seras-Franzoso, Joaquin
AU - Peebo, Karl
AU - García-Fruitós, Elena
AU - Vázquez, Esther
AU - Rinas, Ursula
AU - Villaverde, Antonio
N1 - Funding Information: We are indebted to MINECO ( BFU2010-17450 ), AGAUR ( 2009SGR-0108 ) and CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, Spain) for funding our research on inclusion bodies. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. We also acknowledge technical assistance from the Servei de Cultius Cellulars, Producció d’Anticossos i Citometria (SCAC) at the Universitat Autònoma de Barcelona (UAB). A.V. received an ICREA ACADEMIA award. We are also indebted to the Protein Production Platform (CIBER-BBN) for helpful technical assistance and for protein production and purification services ( http://www.bbn.ciber-bbn.es/programas/plataformas/equipamiento ).
PY - 2014/3
Y1 - 2014/3
N2 - Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37 C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25 C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
AB - Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37 C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25 C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
KW - Drug delivery
KW - Inclusion bodies
KW - Nanomedicine
KW - Protein release
KW - Surface functionalization
UR - http://www.scopus.com/inward/record.url?scp=84895068935&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2013.12.021
DO - 10.1016/j.actbio.2013.12.021
M3 - Article
C2 - 24361427
AN - SCOPUS:84895068935
VL - 10
SP - 1354
EP - 1359
JO - Acta biomaterialia
JF - Acta biomaterialia
SN - 1742-7061
IS - 3
ER -