Imaging of the mouse lung with scanning laser optical tomography (SLOT)

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Manuela Kellner
  • Marko Heidrich
  • Rebecca Beigel
  • Raoul Amadeus Lorbeer
  • Lars Knudsen
  • Tammo Ripken
  • Alexander Heisterkamp
  • Heiko Meyer
  • Mark Philipp Kühnel
  • Matthias Ochs

External Research Organisations

  • Hannover Medical School (MHH)
  • Laser Zentrum Hannover e.V. (LZH)
  • German Center for Lung Research
  • Friedrich Schiller University Jena
  • REBIRTH Research Center for Translational Regenerative Medicine
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Details

Original languageEnglish
Pages (from-to)975-983
Number of pages9
JournalJournal of Applied Physiology
Volume113
Issue number6
Publication statusPublished - 15 Sept 2012
Externally publishedYes

Abstract

The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.

Keywords

    3D microscopy, Acini, Airways, Alveoli, Blood vessels, Optical projection tomography

ASJC Scopus subject areas

Cite this

Imaging of the mouse lung with scanning laser optical tomography (SLOT). / Kellner, Manuela; Heidrich, Marko; Beigel, Rebecca et al.
In: Journal of Applied Physiology, Vol. 113, No. 6, 15.09.2012, p. 975-983.

Research output: Contribution to journalArticleResearchpeer review

Kellner, M, Heidrich, M, Beigel, R, Lorbeer, RA, Knudsen, L, Ripken, T, Heisterkamp, A, Meyer, H, Kühnel, MP & Ochs, M 2012, 'Imaging of the mouse lung with scanning laser optical tomography (SLOT)', Journal of Applied Physiology, vol. 113, no. 6, pp. 975-983. https://doi.org/10.1152/japplphysiol.00026.2012
Kellner, M., Heidrich, M., Beigel, R., Lorbeer, R. A., Knudsen, L., Ripken, T., Heisterkamp, A., Meyer, H., Kühnel, M. P., & Ochs, M. (2012). Imaging of the mouse lung with scanning laser optical tomography (SLOT). Journal of Applied Physiology, 113(6), 975-983. https://doi.org/10.1152/japplphysiol.00026.2012
Kellner M, Heidrich M, Beigel R, Lorbeer RA, Knudsen L, Ripken T et al. Imaging of the mouse lung with scanning laser optical tomography (SLOT). Journal of Applied Physiology. 2012 Sept 15;113(6):975-983. doi: 10.1152/japplphysiol.00026.2012
Kellner, Manuela ; Heidrich, Marko ; Beigel, Rebecca et al. / Imaging of the mouse lung with scanning laser optical tomography (SLOT). In: Journal of Applied Physiology. 2012 ; Vol. 113, No. 6. pp. 975-983.
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