Details
| Original language | English |
|---|---|
| Article number | 2771 |
| Journal | International Journal of Molecular Sciences |
| Volume | 23 |
| Issue number | 5 |
| Publication status | Published - 2 Mar 2022 |
Abstract
The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of poly-myxin binding, were developed and carefully investigated. Characterization of the hybrid nanopar-ticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both pol-ymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudo-monas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the poly-myxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.
Keywords
- Antibiotics, Antimicrobial properties, Colistin, Composite materials, Core–shell structures, Drug delivery systems, Hybrid nanoparticles, Peptides, Polymyxin
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Spectroscopy
- Computer Science(all)
- Computer Science Applications
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
- Chemistry(all)
- Inorganic Chemistry
Sustainable Development Goals
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In: International Journal of Molecular Sciences, Vol. 23, No. 5, 2771, 02.03.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics
AU - Iudin, Dmitrii
AU - Vasilieva, Marina
AU - Knyazeva, Elena
AU - Korzhikov-Vlakh, Viktor
AU - Demyanova, Elena
AU - Lavrentieva, Antonina
AU - Skorik, Yury
AU - Korzhikova-Vlakh, Evgenia
N1 - Funding Information: This research was funded by the Russian Science Foundation (project no. 19-73-20157).
PY - 2022/3/2
Y1 - 2022/3/2
N2 - The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of poly-myxin binding, were developed and carefully investigated. Characterization of the hybrid nanopar-ticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both pol-ymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudo-monas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the poly-myxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.
AB - The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of poly-myxin binding, were developed and carefully investigated. Characterization of the hybrid nanopar-ticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both pol-ymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudo-monas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the poly-myxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.
KW - Antibiotics
KW - Antimicrobial properties
KW - Colistin
KW - Composite materials
KW - Core–shell structures
KW - Drug delivery systems
KW - Hybrid nanoparticles
KW - Peptides
KW - Polymyxin
UR - http://www.scopus.com/inward/record.url?scp=85125417388&partnerID=8YFLogxK
U2 - 10.3390/ijms23052771
DO - 10.3390/ijms23052771
M3 - Article
C2 - 35269910
AN - SCOPUS:85125417388
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 5
M1 - 2771
ER -