Details
| Original language | English |
|---|---|
| Article number | e202100352 |
| Journal | Journal of biophotonics |
| Volume | 15 |
| Issue number | 7 |
| Early online date | 9 Apr 2022 |
| Publication status | Published - Jul 2022 |
Abstract
All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.
Keywords
- calcium imaging, cardiac electrophysiology, channelrhodopsin, optogenetics, spatial light modulator, wavefront shaping
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: Journal of biophotonics, Vol. 15, No. 7, e202100352, 07.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology
AU - Junge, Sebastian
AU - Schmieder, Felix
AU - Sasse, Philipp
AU - Czarske, Jürgen
AU - Torres-Mapa, Maria Leilani
AU - Heisterkamp, Alexander
N1 - Funding Information: We acknowledge the support from the Federal Ministry of Education and Research, Germany (13N14085, Alexander Heisterkamp and Philipp Sasse). This study was partly supported by the German Research Foundation, Germany Clusters of Excellence: REBIRTH (EXC 62) and Hearing4all (EXC 2177) (Alexander Heisterkamp), Physics of Life (2068) (Jürgen Czarske) and German Research Foundation, project CZ55/39 (Jürgen Czarske). Maria Leilani Torres‐Mapa acknowledges the support of Caroline Herschel Program from the Hochschulbüro für Chancenvielfalt, Leibniz University Hannover. Open Access funding enabled and organized by Projekt DEAL.
PY - 2022/7
Y1 - 2022/7
N2 - All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.
AB - All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin-2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal-630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.
KW - calcium imaging
KW - cardiac electrophysiology
KW - channelrhodopsin
KW - optogenetics
KW - spatial light modulator
KW - wavefront shaping
UR - http://www.scopus.com/inward/record.url?scp=85129169794&partnerID=8YFLogxK
U2 - 10.1002/jbio.202100352
DO - 10.1002/jbio.202100352
M3 - Article
C2 - 35397155
AN - SCOPUS:85129169794
VL - 15
JO - Journal of biophotonics
JF - Journal of biophotonics
SN - 1864-063X
IS - 7
M1 - e202100352
ER -