Details
| Original language | English |
|---|---|
| Article number | 63 |
| Journal | Molecular breeding |
| Volume | 45 |
| Issue number | 8 |
| Publication status | Published - 22 Jul 2025 |
Abstract
The potato cyst nematode Globodera pallida is a major agricultural pest that is responsible for significant potato yield losses on a global scale. The most effective and sustainable strategy to reduce damage caused by G. pallida is to cultivate nematode-resistant potato varieties. These varieties have been developed mainly by introgressing resistance genes from wild potato species. However, intensive reliance on limited and widely utilised sources of resistance against the predominant G. pallida pathotypes 2 and 3 (Pa2/3) has led to the emergence of virulent nematode populations that can overcome these defences. To address this issue, we undertook a comprehensive search for novel sources of resistance against this new virulent G. pallida type, ‘Emsland’, within wild Solanum species and identified several promising candidates. Three resistant accessions were further characterised with resistance loci mapped via a single-nucleotide polymorphism array in biparental progenies. Furthermore, one population was subjected to fine-mapping through genotyping-by-sequencing. A major resistance locus was identified in each of the three studied accessions: in a clone of Solanum gourlayi ssp. pachytrichum, resistance was mapped to the short arm of chromosome 5, whereas in clones of both S. hawkesianum and S. spegazzinii, resistance was traced to a locus on the long arm of chromosome 6. This study is the first to identify resistance loci controlling the new virulent G. pallida type, ‘Emsland’ (population ‘Oberlangen’). Our findings provide essential data for the introgression of these resistance loci into cultivated potato varieties, offering the potential to increase nematode resistance in potato crops.
Keywords
- Genebank, Genetic resources, Germplasm, Pyramiding, Quantitative resistance loci, Sustainable crop protection
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Molecular breeding, Vol. 45, No. 8, 63, 22.07.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Getting help from the extended family
T2 - identification and genetic characterisation of novel resistance to Globodera pallida ‘Oberlangen’ in wild Solanum species
AU - Kaufmann, Helgard
AU - Kiewnick, Sebastian
AU - Hammann, Thilo
AU - Tacke, Eckhard
AU - Hartje, Stefanie
AU - Kauder, Friedrich
AU - Muders, Katja
AU - Prigge, Vanessa
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Publisher Copyright: © The Author(s) 2025.
PY - 2025/7/22
Y1 - 2025/7/22
N2 - The potato cyst nematode Globodera pallida is a major agricultural pest that is responsible for significant potato yield losses on a global scale. The most effective and sustainable strategy to reduce damage caused by G. pallida is to cultivate nematode-resistant potato varieties. These varieties have been developed mainly by introgressing resistance genes from wild potato species. However, intensive reliance on limited and widely utilised sources of resistance against the predominant G. pallida pathotypes 2 and 3 (Pa2/3) has led to the emergence of virulent nematode populations that can overcome these defences. To address this issue, we undertook a comprehensive search for novel sources of resistance against this new virulent G. pallida type, ‘Emsland’, within wild Solanum species and identified several promising candidates. Three resistant accessions were further characterised with resistance loci mapped via a single-nucleotide polymorphism array in biparental progenies. Furthermore, one population was subjected to fine-mapping through genotyping-by-sequencing. A major resistance locus was identified in each of the three studied accessions: in a clone of Solanum gourlayi ssp. pachytrichum, resistance was mapped to the short arm of chromosome 5, whereas in clones of both S. hawkesianum and S. spegazzinii, resistance was traced to a locus on the long arm of chromosome 6. This study is the first to identify resistance loci controlling the new virulent G. pallida type, ‘Emsland’ (population ‘Oberlangen’). Our findings provide essential data for the introgression of these resistance loci into cultivated potato varieties, offering the potential to increase nematode resistance in potato crops.
AB - The potato cyst nematode Globodera pallida is a major agricultural pest that is responsible for significant potato yield losses on a global scale. The most effective and sustainable strategy to reduce damage caused by G. pallida is to cultivate nematode-resistant potato varieties. These varieties have been developed mainly by introgressing resistance genes from wild potato species. However, intensive reliance on limited and widely utilised sources of resistance against the predominant G. pallida pathotypes 2 and 3 (Pa2/3) has led to the emergence of virulent nematode populations that can overcome these defences. To address this issue, we undertook a comprehensive search for novel sources of resistance against this new virulent G. pallida type, ‘Emsland’, within wild Solanum species and identified several promising candidates. Three resistant accessions were further characterised with resistance loci mapped via a single-nucleotide polymorphism array in biparental progenies. Furthermore, one population was subjected to fine-mapping through genotyping-by-sequencing. A major resistance locus was identified in each of the three studied accessions: in a clone of Solanum gourlayi ssp. pachytrichum, resistance was mapped to the short arm of chromosome 5, whereas in clones of both S. hawkesianum and S. spegazzinii, resistance was traced to a locus on the long arm of chromosome 6. This study is the first to identify resistance loci controlling the new virulent G. pallida type, ‘Emsland’ (population ‘Oberlangen’). Our findings provide essential data for the introgression of these resistance loci into cultivated potato varieties, offering the potential to increase nematode resistance in potato crops.
KW - Genebank
KW - Genetic resources
KW - Germplasm
KW - Pyramiding
KW - Quantitative resistance loci
KW - Sustainable crop protection
UR - http://www.scopus.com/inward/record.url?scp=105011355205&partnerID=8YFLogxK
U2 - 10.1007/s11032-025-01582-0
DO - 10.1007/s11032-025-01582-0
M3 - Article
VL - 45
JO - Molecular breeding
JF - Molecular breeding
SN - 1380-3743
IS - 8
M1 - 63
ER -