Details
| Original language | English |
|---|---|
| Pages (from-to) | 119-126 |
| Number of pages | 8 |
| Journal | Food and chemical toxicology |
| Volume | 73 |
| Publication status | Published - 1 Nov 2014 |
Abstract
Betanin is a red pigment present in red beetroot. Recently, potential health benefits of betanin-rich beetroot have been suggested. However, little is known regarding the free radical scavenging and antioxidant activity of betanin. Electron spin resonance spectroscopy (ESR) and spin trapping techniques were applied to evaluate the ability of betanin to scavenge hydroxyl, superoxide, 2,2 diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl free radicals. In addition, we tested in cultured cells the ability of betanin to prevent DNA damage and to induce the transcription factor Nrf2 (nuclear factor (erythroid-derived 2)-like 2) as well as its down-stream target heme oxygenase. 1 (HO-1), paraoxonase. 1 (PON1) and glutathione (GSH). Betanin dose-dependently scavenged DPPH-, galvinoxyl-, superoxide-, and hydroxyl-radicals in the ESR and spin trapping studies and prevented hydrogen peroxide induced DNA damage as determined by the Comet assay. Furthermore, betanin treatment induced the transcription factor Nrf2 and resulted in an increase of HO-1 protein levels, PON1-transactivation and cellular GSH. Present data suggest that betanin is both a free radical scavenger and an inducer of antioxidant defense mechanism in cultured cells.
Keywords
- Beetroot, Betanin, ESR, Heme oxygenase 1, Nrf2, Paraoxonase 1
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Pharmacology, Toxicology and Pharmaceutics(all)
- Toxicology
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In: Food and chemical toxicology, Vol. 73, 01.11.2014, p. 119-126.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells
AU - Esatbeyoglu, T.
AU - Wagner, A.E.
AU - Motafakkerazad, R.
AU - Nakajima, Y.
AU - Matsugo, S.
AU - Rimbach, G.
N1 - Publisher Copyright: © 2014 Elsevier Ltd. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Betanin is a red pigment present in red beetroot. Recently, potential health benefits of betanin-rich beetroot have been suggested. However, little is known regarding the free radical scavenging and antioxidant activity of betanin. Electron spin resonance spectroscopy (ESR) and spin trapping techniques were applied to evaluate the ability of betanin to scavenge hydroxyl, superoxide, 2,2 diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl free radicals. In addition, we tested in cultured cells the ability of betanin to prevent DNA damage and to induce the transcription factor Nrf2 (nuclear factor (erythroid-derived 2)-like 2) as well as its down-stream target heme oxygenase. 1 (HO-1), paraoxonase. 1 (PON1) and glutathione (GSH). Betanin dose-dependently scavenged DPPH-, galvinoxyl-, superoxide-, and hydroxyl-radicals in the ESR and spin trapping studies and prevented hydrogen peroxide induced DNA damage as determined by the Comet assay. Furthermore, betanin treatment induced the transcription factor Nrf2 and resulted in an increase of HO-1 protein levels, PON1-transactivation and cellular GSH. Present data suggest that betanin is both a free radical scavenger and an inducer of antioxidant defense mechanism in cultured cells.
AB - Betanin is a red pigment present in red beetroot. Recently, potential health benefits of betanin-rich beetroot have been suggested. However, little is known regarding the free radical scavenging and antioxidant activity of betanin. Electron spin resonance spectroscopy (ESR) and spin trapping techniques were applied to evaluate the ability of betanin to scavenge hydroxyl, superoxide, 2,2 diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl free radicals. In addition, we tested in cultured cells the ability of betanin to prevent DNA damage and to induce the transcription factor Nrf2 (nuclear factor (erythroid-derived 2)-like 2) as well as its down-stream target heme oxygenase. 1 (HO-1), paraoxonase. 1 (PON1) and glutathione (GSH). Betanin dose-dependently scavenged DPPH-, galvinoxyl-, superoxide-, and hydroxyl-radicals in the ESR and spin trapping studies and prevented hydrogen peroxide induced DNA damage as determined by the Comet assay. Furthermore, betanin treatment induced the transcription factor Nrf2 and resulted in an increase of HO-1 protein levels, PON1-transactivation and cellular GSH. Present data suggest that betanin is both a free radical scavenger and an inducer of antioxidant defense mechanism in cultured cells.
KW - Beetroot
KW - Betanin
KW - ESR
KW - Heme oxygenase 1
KW - Nrf2
KW - Paraoxonase 1
UR - http://www.scopus.com/inward/record.url?scp=84908070360&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2014.08.007
DO - 10.1016/j.fct.2014.08.007
M3 - Article
VL - 73
SP - 119
EP - 126
JO - Food and chemical toxicology
JF - Food and chemical toxicology
SN - 0278-6915
ER -