Details
| Original language | English |
|---|---|
| Article number | 714 |
| Journal | Frontiers in Bioengineering and Biotechnology |
| Volume | 8 |
| Publication status | Published - 7 Jul 2020 |
| Externally published | Yes |
Abstract
In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.
Keywords
- biocatalysis, enzymatic reactions, heterogeneous catalysis, metal–organic frameworks, size-selective catalysis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Histology
- Engineering(all)
- Biomedical Engineering
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In: Frontiers in Bioengineering and Biotechnology, Vol. 8, 714, 07.07.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
AU - Wang, Yangxin
AU - Zhang, Ningning
AU - Tan, Deming
AU - Qi, Zhenhui
AU - Wu, Changzhu
N1 - Funding Information: We thank Prof. Marion B. Ansorge-Schumacher (TU Dresden) for valuable discussions and support and Prof. Rainer Jordan (TU Dresden) for providing the lab facility. Funding. We acknowledge financial support from the Deutsche Forschungsgemeinschaft (WU 814/1-1). This work was also financially supported by the Thousand Talents Program of China (1800-16GH030121), Key R&D Program of Shaanxi Province (2019KW-031 and 2019KW-038), China Postdoctoral Science Foundation (2017M623231), and Fundamental Research Funds for the Central Universities (3102018zy051).
PY - 2020/7/7
Y1 - 2020/7/7
N2 - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.
AB - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.
KW - biocatalysis
KW - enzymatic reactions
KW - heterogeneous catalysis
KW - metal–organic frameworks
KW - size-selective catalysis
UR - http://www.scopus.com/inward/record.url?scp=85088468868&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2020.00714
DO - 10.3389/fbioe.2020.00714
M3 - Article
AN - SCOPUS:85088468868
VL - 8
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
SN - 2296-4185
M1 - 714
ER -