Details
| Original language | English |
|---|---|
| Article number | e202500794 |
| Journal | CHEMBIOCHEM |
| Volume | 27 |
| Issue number | 1 |
| Publication status | Published - 8 Jan 2026 |
Abstract
The actin cytoskeleton plays a central role in cellular organization and dynamics, yet the tools available for targeting actin function remain limited. Cytochalasans represent a large class of actin-targeting natural products that are readily cell permeable with varying cytotoxicity and actin-targeting capabilities in mammalian cells. Their pharmacologic exploitation not only requires an understanding of their mode of action but also exact knowledge of how they can be derivatized without affecting their bioactivity. Herein, the design, synthesis, and evaluation of five fluorescently labeled [11]cytochalasan derivatives generated from pyrichalasin H (PyriH) and 19,20-epoxycytochalasin C (EpoxyCytoC) are reported. Guided by molecular docking, the C21-OAc moiety is identified as a promising site for tag attachment without disrupting actin binding. Semisynthetic modifications enable the conjugation of different dyes to PyriH and EpoxyCytoC scaffolds. Moreover, the effect of linkers separating cytochalasans and dyes is analyzed. Biological evaluation supplemented by in vitro assays to additionally interrogates their activities on the assembly of pure actin filaments revealing distinct activity profiles. Together, this study compares permeability, cytotoxicity, and actin binding of five novel [11]cytochalasan probes bearing substitutions of the C21-OAc moiety. These findings establish the C21-OAc position as a versatile functionalization site and provide a framework for developing next generation cytochalasan-based actin-targeting probes.
Keywords
- actin imaging, molecular docking, semisynthesis, [11]cytochalsans
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Organic Chemistry
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In: CHEMBIOCHEM, Vol. 27, No. 1, e202500794, 08.01.2026.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Exploring the Substitution of the C21-OAc Moiety in [11]Cytochalasans
T2 - Synthesis and Evaluation of Fluorophore Conjugates
AU - Kagho, Mervic D.
AU - Schmidt, Katharina
AU - Chakrabarti, Aishi
AU - Karger, Marius
AU - Lambert, Christopher
AU - Hauser, Maurice
AU - Cox, Russell J.
AU - Rottner, Klemens
AU - Stadler, Marc
AU - Stradal, Theresia E.
AU - Klahn, Philipp
N1 - Publisher Copyright: © 2025 The Author(s). ChemBioChem published by Wiley-VCH GmbH.
PY - 2026/1/8
Y1 - 2026/1/8
N2 - The actin cytoskeleton plays a central role in cellular organization and dynamics, yet the tools available for targeting actin function remain limited. Cytochalasans represent a large class of actin-targeting natural products that are readily cell permeable with varying cytotoxicity and actin-targeting capabilities in mammalian cells. Their pharmacologic exploitation not only requires an understanding of their mode of action but also exact knowledge of how they can be derivatized without affecting their bioactivity. Herein, the design, synthesis, and evaluation of five fluorescently labeled [11]cytochalasan derivatives generated from pyrichalasin H (PyriH) and 19,20-epoxycytochalasin C (EpoxyCytoC) are reported. Guided by molecular docking, the C21-OAc moiety is identified as a promising site for tag attachment without disrupting actin binding. Semisynthetic modifications enable the conjugation of different dyes to PyriH and EpoxyCytoC scaffolds. Moreover, the effect of linkers separating cytochalasans and dyes is analyzed. Biological evaluation supplemented by in vitro assays to additionally interrogates their activities on the assembly of pure actin filaments revealing distinct activity profiles. Together, this study compares permeability, cytotoxicity, and actin binding of five novel [11]cytochalasan probes bearing substitutions of the C21-OAc moiety. These findings establish the C21-OAc position as a versatile functionalization site and provide a framework for developing next generation cytochalasan-based actin-targeting probes.
AB - The actin cytoskeleton plays a central role in cellular organization and dynamics, yet the tools available for targeting actin function remain limited. Cytochalasans represent a large class of actin-targeting natural products that are readily cell permeable with varying cytotoxicity and actin-targeting capabilities in mammalian cells. Their pharmacologic exploitation not only requires an understanding of their mode of action but also exact knowledge of how they can be derivatized without affecting their bioactivity. Herein, the design, synthesis, and evaluation of five fluorescently labeled [11]cytochalasan derivatives generated from pyrichalasin H (PyriH) and 19,20-epoxycytochalasin C (EpoxyCytoC) are reported. Guided by molecular docking, the C21-OAc moiety is identified as a promising site for tag attachment without disrupting actin binding. Semisynthetic modifications enable the conjugation of different dyes to PyriH and EpoxyCytoC scaffolds. Moreover, the effect of linkers separating cytochalasans and dyes is analyzed. Biological evaluation supplemented by in vitro assays to additionally interrogates their activities on the assembly of pure actin filaments revealing distinct activity profiles. Together, this study compares permeability, cytotoxicity, and actin binding of five novel [11]cytochalasan probes bearing substitutions of the C21-OAc moiety. These findings establish the C21-OAc position as a versatile functionalization site and provide a framework for developing next generation cytochalasan-based actin-targeting probes.
KW - actin imaging
KW - molecular docking
KW - semisynthesis
KW - [11]cytochalsans
UR - http://www.scopus.com/inward/record.url?scp=105023098709&partnerID=8YFLogxK
U2 - 10.1002/cbic.202500794
DO - 10.1002/cbic.202500794
M3 - Article
AN - SCOPUS:105023098709
VL - 27
JO - CHEMBIOCHEM
JF - CHEMBIOCHEM
SN - 1439-4227
IS - 1
M1 - e202500794
ER -