Enzymatic degradation of the antibiotic sulfamethazine by using crude extracts of different halophytic plants

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Original languageEnglish
Pages (from-to)1104-1111
Number of pages8
JournalInternational Journal of Phytoremediation
Volume21
Issue number11
Early online date30 Apr 2019
Publication statusPublished - 19 Sept 2019

Abstract

The biodegradation of the antibiotic sulfamethazine (SMT) by using different crude extracts of halophytes was investigated. For this purpose, crude water extracts of the halophytes Chenopodium quinoa, Sesuvium portulacastrum, and Tripolium pannonicum were prepared. Different amounts of SMT were added to the different extracts (final concentration of 1, 2, and 5 mg L−1) and incubated at 37 °C. Crude extracts of T. pannonicum were further used to evaluate the degradation rate over time. In order to evaluate the influence of endophytic or naturally plant-associated microorganisms on the biodegradation of SMT, extracts from plants grown in sterile and non-sterile conditions were compared. SMT was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, crude extracts of T. pannonicum have a high potential to biodegrade SMT with a decrease up to 85.4% (4.27 ± 0.10 mg L−1) from an initial concentration of 5 mg L−1. The lowest activity was obtained using extracts of C. quinoa with degradation of 4.5%. Extracts of plants cultivated under sterile and non-sterile conditions do not have any significant difference in SMT degradation. Therefore, microorganisms and their enzymatic activities do not seem to play a significant role during this process.

Keywords

    Enzymatic assay, halophyte plants, phytoremediation, sulfamethazine biodegradation

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Enzymatic degradation of the antibiotic sulfamethazine by using crude extracts of different halophytic plants. / Turcios Pantaleón, Ariel Eliseo; Papenbrock, Jutta.
In: International Journal of Phytoremediation, Vol. 21, No. 11, 19.09.2019, p. 1104-1111.

Research output: Contribution to journalArticleResearchpeer review

Turcios Pantaleón AE, Papenbrock J. Enzymatic degradation of the antibiotic sulfamethazine by using crude extracts of different halophytic plants. International Journal of Phytoremediation. 2019 Sept 19;21(11):1104-1111. Epub 2019 Apr 30. doi: 10.1080/15226514.2019.1606782
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abstract = "The biodegradation of the antibiotic sulfamethazine (SMT) by using different crude extracts of halophytes was investigated. For this purpose, crude water extracts of the halophytes Chenopodium quinoa, Sesuvium portulacastrum, and Tripolium pannonicum were prepared. Different amounts of SMT were added to the different extracts (final concentration of 1, 2, and 5 mg L−1) and incubated at 37 °C. Crude extracts of T. pannonicum were further used to evaluate the degradation rate over time. In order to evaluate the influence of endophytic or naturally plant-associated microorganisms on the biodegradation of SMT, extracts from plants grown in sterile and non-sterile conditions were compared. SMT was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, crude extracts of T. pannonicum have a high potential to biodegrade SMT with a decrease up to 85.4% (4.27 ± 0.10 mg L−1) from an initial concentration of 5 mg L−1. The lowest activity was obtained using extracts of C. quinoa with degradation of 4.5%. Extracts of plants cultivated under sterile and non-sterile conditions do not have any significant difference in SMT degradation. Therefore, microorganisms and their enzymatic activities do not seem to play a significant role during this process.",
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