Details
| Original language | English |
|---|---|
| Pages (from-to) | 812-830 |
| Number of pages | 19 |
| Journal | Plant Cell and Environment |
| Volume | 46 |
| Issue number | 3 |
| Early online date | 20 Dec 2022 |
| Publication status | Published - 1 Feb 2023 |
Abstract
Elevated CO2 (eCO2) reduces the impact of drought, but the mechanisms underlying this effect remain unclear. Therefore, we used a multidisciplinary approach to investigate the interaction of drought and eCO2 in Arabidopsis thaliana leaves. Transcriptome and subsequent metabolite analyses identified a strong induction of the aliphatic glucosinolate (GL) biosynthesis as a main effect of eCO2 in drought-stressed leaves. Transcriptome results highlighted the upregulation of ABI5 and downregulation of WRKY63 transcription factors (TF), known to enhance and inhibit the expression of genes regulating aliphatic GL biosynthesis (e.g., MYB28 and 29 TFs), respectively. In addition, eCO2 positively regulated aliphatic GL biosynthesis by MYB28/29 and increasing the accumulation of GL precursors. To test the role of GLs in the stress-mitigating effect of eCO2, we investigated the effect of genetic perturbations of the GL biosynthesis. Overexpression of MYB28, 29 and 76 improved drought tolerance by inducing stomatal closure and maintaining plant turgor, whereas loss of cyp79f genes reduced the stress-mitigating effect of eCO2 and decreased drought tolerance. Overall, the crucial role of GL metabolism in drought stress mitigation by eCO2 could be a beneficial trait to overcome future climate challenges.
Keywords
- Arabidopsis thaliana, climate change, glucosinolate metabolism, hormonal signaling, stomatal conductance
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
Sustainable Development Goals
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In: Plant Cell and Environment, Vol. 46, No. 3, 01.02.2023, p. 812-830.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Elevated CO2 mitigates the impact of drought stress by upregulating glucosinolate metabolism in Arabidopsis thaliana
AU - AbdElgawad, Hamada
AU - Zinta, Gaurav
AU - Hornbacher, Johann
AU - Papenbrock, Jutta
AU - Markakis, Marios N.
AU - Asard, Han
AU - Beemster, Gerrit T.S.
N1 - Funding Information: This research was funded by the Research Council of the University of Antwerp as concerted research project ‘Changes in the stress sensitivity of plants and ecosystems under climate change conditions’ (GOA‐BOF‐UA‐2007).
PY - 2023/2/1
Y1 - 2023/2/1
N2 - Elevated CO2 (eCO2) reduces the impact of drought, but the mechanisms underlying this effect remain unclear. Therefore, we used a multidisciplinary approach to investigate the interaction of drought and eCO2 in Arabidopsis thaliana leaves. Transcriptome and subsequent metabolite analyses identified a strong induction of the aliphatic glucosinolate (GL) biosynthesis as a main effect of eCO2 in drought-stressed leaves. Transcriptome results highlighted the upregulation of ABI5 and downregulation of WRKY63 transcription factors (TF), known to enhance and inhibit the expression of genes regulating aliphatic GL biosynthesis (e.g., MYB28 and 29 TFs), respectively. In addition, eCO2 positively regulated aliphatic GL biosynthesis by MYB28/29 and increasing the accumulation of GL precursors. To test the role of GLs in the stress-mitigating effect of eCO2, we investigated the effect of genetic perturbations of the GL biosynthesis. Overexpression of MYB28, 29 and 76 improved drought tolerance by inducing stomatal closure and maintaining plant turgor, whereas loss of cyp79f genes reduced the stress-mitigating effect of eCO2 and decreased drought tolerance. Overall, the crucial role of GL metabolism in drought stress mitigation by eCO2 could be a beneficial trait to overcome future climate challenges.
AB - Elevated CO2 (eCO2) reduces the impact of drought, but the mechanisms underlying this effect remain unclear. Therefore, we used a multidisciplinary approach to investigate the interaction of drought and eCO2 in Arabidopsis thaliana leaves. Transcriptome and subsequent metabolite analyses identified a strong induction of the aliphatic glucosinolate (GL) biosynthesis as a main effect of eCO2 in drought-stressed leaves. Transcriptome results highlighted the upregulation of ABI5 and downregulation of WRKY63 transcription factors (TF), known to enhance and inhibit the expression of genes regulating aliphatic GL biosynthesis (e.g., MYB28 and 29 TFs), respectively. In addition, eCO2 positively regulated aliphatic GL biosynthesis by MYB28/29 and increasing the accumulation of GL precursors. To test the role of GLs in the stress-mitigating effect of eCO2, we investigated the effect of genetic perturbations of the GL biosynthesis. Overexpression of MYB28, 29 and 76 improved drought tolerance by inducing stomatal closure and maintaining plant turgor, whereas loss of cyp79f genes reduced the stress-mitigating effect of eCO2 and decreased drought tolerance. Overall, the crucial role of GL metabolism in drought stress mitigation by eCO2 could be a beneficial trait to overcome future climate challenges.
KW - Arabidopsis thaliana
KW - climate change
KW - glucosinolate metabolism
KW - hormonal signaling
KW - stomatal conductance
UR - http://www.scopus.com/inward/record.url?scp=85146313653&partnerID=8YFLogxK
U2 - 10.1111/pce.14521
DO - 10.1111/pce.14521
M3 - Article
AN - SCOPUS:85146313653
VL - 46
SP - 812
EP - 830
JO - Plant Cell and Environment
JF - Plant Cell and Environment
SN - 0140-7791
IS - 3
ER -