Details
Original language | English |
---|---|
Pages (from-to) | 1913-1923 |
Number of pages | 11 |
Journal | Journal of Animal Science |
Volume | 94 |
Issue number | 5 |
Publication status | Published - May 2016 |
Externally published | Yes |
Abstract
The purpose of this study was to evaluate possible effects of quercetin (Q) on liver lipid metabolism and antioxidative status in periparturient dairy cows. The periparturient period is associated with enormous metabolic changes for dairy cows. Energy needs for incipient lactation are too high to be balanced by feed intake, leading to negative energy balance and body fat mobilization. It has been estimated that this leads to the development of fatty liver in about 50% of cows, which are at high risk for disease. Furthermore, the antioxidative status of these cows may be impaired. Quercetin is a plant flavonoid having hepatoprotective and antioxidative potential and the ability to reduce liver lipid accumulation in monogastric animals. Little information is available in regard to these effects in ruminants. To prevent microbial Q degradation in the rumen, Q was administered via a duodenal fistula to improve systemic availability. Five cows of the Q-treated group received, daily, 100 mg of quercetin dehydrate/ kg BW in a 0.9% sodium chloride solution from d −20 until d 20 relative to calving, whereas 5 control (CTR) cows received only a sodium chloride solution. Blood samples were taken weekly and liver biopsies were performed in wk −4, −2, and 3 relative to calving. Cows treated with Q showed a tendency (P = 0.082) for lower liver fat content compared with CTR cows. Liver glycogen, glutathione concentrations, and relative mRNA abundance of genes related to hepatic lipid metabolism and antioxidative status as well as parameters of antioxidative status in plasma were not affected (P > 0.1) by Q supplementation. In conclusion, liver fat content in dairy cows tended to be reduced by Q supplementation, but potential underlying mechanisms remain unclear because analyzed parameters related to hepatic lipid metabolism and antioxidative defense were not altered by Q supplementation.
Keywords
- Antioxidative status, Flavonoid, Liver fat, Metabolism, Quercetin, Transition dairy cow
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Agricultural and Biological Sciences(all)
- Animal Science and Zoology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Journal of Animal Science, Vol. 94, No. 5, 05.2016, p. 1913-1923.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effects of a six-week intraduodenal supplementation with quercetin on liver lipid metabolism and oxidative stress in peripartal dairy cows
AU - Stoldt, A. K.
AU - Mielenz, M.
AU - Nürnberg, G.
AU - Sauerwein, H.
AU - Esatbeyoglu, T.
AU - Wagner, A. E.
AU - Rimbach, G.
AU - Starke, A.
AU - Wolffram, S.
AU - Metges, C. C.
N1 - Publisher Copyright: © 2016 American Society of Animal Science. All rights reserved.
PY - 2016/5
Y1 - 2016/5
N2 - The purpose of this study was to evaluate possible effects of quercetin (Q) on liver lipid metabolism and antioxidative status in periparturient dairy cows. The periparturient period is associated with enormous metabolic changes for dairy cows. Energy needs for incipient lactation are too high to be balanced by feed intake, leading to negative energy balance and body fat mobilization. It has been estimated that this leads to the development of fatty liver in about 50% of cows, which are at high risk for disease. Furthermore, the antioxidative status of these cows may be impaired. Quercetin is a plant flavonoid having hepatoprotective and antioxidative potential and the ability to reduce liver lipid accumulation in monogastric animals. Little information is available in regard to these effects in ruminants. To prevent microbial Q degradation in the rumen, Q was administered via a duodenal fistula to improve systemic availability. Five cows of the Q-treated group received, daily, 100 mg of quercetin dehydrate/ kg BW in a 0.9% sodium chloride solution from d −20 until d 20 relative to calving, whereas 5 control (CTR) cows received only a sodium chloride solution. Blood samples were taken weekly and liver biopsies were performed in wk −4, −2, and 3 relative to calving. Cows treated with Q showed a tendency (P = 0.082) for lower liver fat content compared with CTR cows. Liver glycogen, glutathione concentrations, and relative mRNA abundance of genes related to hepatic lipid metabolism and antioxidative status as well as parameters of antioxidative status in plasma were not affected (P > 0.1) by Q supplementation. In conclusion, liver fat content in dairy cows tended to be reduced by Q supplementation, but potential underlying mechanisms remain unclear because analyzed parameters related to hepatic lipid metabolism and antioxidative defense were not altered by Q supplementation.
AB - The purpose of this study was to evaluate possible effects of quercetin (Q) on liver lipid metabolism and antioxidative status in periparturient dairy cows. The periparturient period is associated with enormous metabolic changes for dairy cows. Energy needs for incipient lactation are too high to be balanced by feed intake, leading to negative energy balance and body fat mobilization. It has been estimated that this leads to the development of fatty liver in about 50% of cows, which are at high risk for disease. Furthermore, the antioxidative status of these cows may be impaired. Quercetin is a plant flavonoid having hepatoprotective and antioxidative potential and the ability to reduce liver lipid accumulation in monogastric animals. Little information is available in regard to these effects in ruminants. To prevent microbial Q degradation in the rumen, Q was administered via a duodenal fistula to improve systemic availability. Five cows of the Q-treated group received, daily, 100 mg of quercetin dehydrate/ kg BW in a 0.9% sodium chloride solution from d −20 until d 20 relative to calving, whereas 5 control (CTR) cows received only a sodium chloride solution. Blood samples were taken weekly and liver biopsies were performed in wk −4, −2, and 3 relative to calving. Cows treated with Q showed a tendency (P = 0.082) for lower liver fat content compared with CTR cows. Liver glycogen, glutathione concentrations, and relative mRNA abundance of genes related to hepatic lipid metabolism and antioxidative status as well as parameters of antioxidative status in plasma were not affected (P > 0.1) by Q supplementation. In conclusion, liver fat content in dairy cows tended to be reduced by Q supplementation, but potential underlying mechanisms remain unclear because analyzed parameters related to hepatic lipid metabolism and antioxidative defense were not altered by Q supplementation.
KW - Antioxidative status
KW - Flavonoid
KW - Liver fat
KW - Metabolism
KW - Quercetin
KW - Transition dairy cow
UR - http://www.scopus.com/inward/record.url?scp=85043409006&partnerID=8YFLogxK
U2 - 10.2527/jas.2016-0338
DO - 10.2527/jas.2016-0338
M3 - Article
C2 - 27285689
VL - 94
SP - 1913
EP - 1923
JO - Journal of Animal Science
JF - Journal of Animal Science
SN - 0021-8812
IS - 5
ER -