Details
| Original language | English |
|---|---|
| Article number | 825 |
| Journal | International Journal of Environmental Research and Public Health |
| Volume | 14 |
| Issue number | 7 |
| Publication status | Published - 22 Jul 2017 |
Abstract
The mycotoxin patulin (PAT) was purified from Penicillium vulpinum CM1 culture that has been isolated from a soil cultivated with maize. The effect of PAT and of a fungal culture filtrate on the activities of glutathione-S-transferase (GST) and some antioxidant enzymes viz. ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) was investigated in roots and shoots of 8-day-old maize seedlings. PAT and culture filtrate caused significant reduction effects in a dose-related manner on the total GST activity. Upon application of the high PAT concentration (25 μg·mL-1) and of the concentrated fungal filtrate (100%, v/v), the reduction in GST activity of roots was 73.8–76.0% and of shoots was 60–61.7%. Conversely, significant increases in the activities of antioxidant enzymes were induced. Application of 25 μg·PAT·mL-1 increased APX, GR, DHAR, and MDHAR activity of root by 2.40-, 2.00-, 1.24-, and 2.16-fold, respectively. In shoots, the enzymatic activity was increased by 1.57-, 1.45-, 1.45-, and 1.61-fold, respectively. Similar induction values of the enzymatic activity were obtained upon application of the concentrated fungal filtrate. This is the first report describing the response of GST and antioxidant enzyme activities of plant cells to PAT toxicity.
Keywords
- Antioxidants, Glutathione-S-transferase (GST), Maize, Patulin (PAT), Penicillium vulpinum
ASJC Scopus subject areas
- Medicine(all)
- Public Health, Environmental and Occupational Health
- Environmental Science(all)
- Health, Toxicology and Mutagenesis
Sustainable Development Goals
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In: International Journal of Environmental Research and Public Health, Vol. 14, No. 7, 825, 22.07.2017.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of patulin from penicillium vulpinum on the activity of Glutathione-S-Transferase and selected antioxidative enzymes in Maize
AU - Ismaiel, Ahmed A.
AU - Papenbrock, Jutta
N1 - Funding information: This work was financially supported by a research grant from the Deutsche Forschungsgemeinschaft (PA 764/19-1, AOBJ 618653). We thank Pamela von Trzebiatowski and Julia Volker for their excellent technical assistance. We also acknowledge Walid Abuelsoud, Institut für Botanik, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany, for his valuable advices concerning measurement of enzymatic activities. The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2017/7/22
Y1 - 2017/7/22
N2 - The mycotoxin patulin (PAT) was purified from Penicillium vulpinum CM1 culture that has been isolated from a soil cultivated with maize. The effect of PAT and of a fungal culture filtrate on the activities of glutathione-S-transferase (GST) and some antioxidant enzymes viz. ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) was investigated in roots and shoots of 8-day-old maize seedlings. PAT and culture filtrate caused significant reduction effects in a dose-related manner on the total GST activity. Upon application of the high PAT concentration (25 μg·mL-1) and of the concentrated fungal filtrate (100%, v/v), the reduction in GST activity of roots was 73.8–76.0% and of shoots was 60–61.7%. Conversely, significant increases in the activities of antioxidant enzymes were induced. Application of 25 μg·PAT·mL-1 increased APX, GR, DHAR, and MDHAR activity of root by 2.40-, 2.00-, 1.24-, and 2.16-fold, respectively. In shoots, the enzymatic activity was increased by 1.57-, 1.45-, 1.45-, and 1.61-fold, respectively. Similar induction values of the enzymatic activity were obtained upon application of the concentrated fungal filtrate. This is the first report describing the response of GST and antioxidant enzyme activities of plant cells to PAT toxicity.
AB - The mycotoxin patulin (PAT) was purified from Penicillium vulpinum CM1 culture that has been isolated from a soil cultivated with maize. The effect of PAT and of a fungal culture filtrate on the activities of glutathione-S-transferase (GST) and some antioxidant enzymes viz. ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) was investigated in roots and shoots of 8-day-old maize seedlings. PAT and culture filtrate caused significant reduction effects in a dose-related manner on the total GST activity. Upon application of the high PAT concentration (25 μg·mL-1) and of the concentrated fungal filtrate (100%, v/v), the reduction in GST activity of roots was 73.8–76.0% and of shoots was 60–61.7%. Conversely, significant increases in the activities of antioxidant enzymes were induced. Application of 25 μg·PAT·mL-1 increased APX, GR, DHAR, and MDHAR activity of root by 2.40-, 2.00-, 1.24-, and 2.16-fold, respectively. In shoots, the enzymatic activity was increased by 1.57-, 1.45-, 1.45-, and 1.61-fold, respectively. Similar induction values of the enzymatic activity were obtained upon application of the concentrated fungal filtrate. This is the first report describing the response of GST and antioxidant enzyme activities of plant cells to PAT toxicity.
KW - Antioxidants
KW - Glutathione-S-transferase (GST)
KW - Maize
KW - Patulin (PAT)
KW - Penicillium vulpinum
UR - http://www.scopus.com/inward/record.url?scp=85026681696&partnerID=8YFLogxK
U2 - 10.3390/ijerph14070825
DO - 10.3390/ijerph14070825
M3 - Article
AN - SCOPUS:85026681696
VL - 14
JO - International Journal of Environmental Research and Public Health
JF - International Journal of Environmental Research and Public Health
SN - 1661-7827
IS - 7
M1 - 825
ER -