Details
| Original language | English |
|---|---|
| Pages (from-to) | 76-87 |
| Number of pages | 12 |
| Journal | Prostaglandins Leukotrienes and Essential Fatty Acids |
| Volume | 121 |
| Publication status | Published - Jun 2017 |
Abstract
INTRODUCTION: EPA and DHA cause different physiological effects, which are in many cases mediated via their oxidative metabolites (oxylipins). However, metabolism studies investigating the effect of either EPA or DHA on comprehensive oxylipin patterns are lacking.
MATERIAL AND METHODS: The short and long term (1, 3, 6, and 12 week) effect of 1076mg/d DHA (free of EPA) on free (unesterified) oxylipin concentrations in plasma and lipopolysacharid (LPS) stimulated blood of 12 healthy men (mean age 25.1 ± 1.5 years) was investigated.
RESULTS: After DHA supplementation, plasma levels of all DHA-oxylipins (HDHAs, EpDPEs, DiHDPEs) significantly increased (up to 600%) in a time-dependent fashion. Oxylipins of EPA and arachidonic acid (AA) were also affected. Whereas a slight increase in several EPA-derived hydroxy-FAs (including the RvE1 precursor 18-HEPE) and dihydroxy-FAs was observed after DHA supplementation, a trend to a slight decline in AA-derived oxylipin levels was found. In LPS stimulated blood, it is shown that DHA supplementation significantly reduces the ability of immune cells to form AA-derived COX (TXB2 and PGB2) and 12-LOX (12-HETE) eicosanoids. While no increase in EPA COX metabolites was found, n-3 PUFA 12-LOX metabolites of EPA (12-HEPE) and DHA (14-HDHA) were highly induced.
CONCLUSION: We demonstrated that DHA supplementation causes a time-dependent shift in the entire oxylipin profile suggesting a cross-linked metabolism of PUFAs and subsequent formation of oxygenated lipid mediators.
Keywords
- 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid/blood, Adult, Arachidonic Acid/metabolism, C-Reactive Protein/metabolism, Dietary Supplements, Docosahexaenoic Acids/administration & dosage, Eicosanoids/metabolism, Fatty Acids, Unsaturated/blood, Germany, Humans, Inflammation/blood, Leukocyte Count, Lipopolysaccharides/toxicity, Male, Oxylipins/blood, Young Adult, Immune cell stimulation, LC n-3 PUFA, EPA, Free oxylipins, DHA, Lipid mediators
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 121, 06.2017, p. 76-87.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of DHA supplementation on oxylipin levels in plasma and immune cell stimulated blood
AU - Schuchardt, Jan Philipp
AU - Ostermann, Annika I
AU - Stork, Lisa
AU - Fritzsch, Sabrina
AU - Kohrs, Heike
AU - Greupner, Theresa
AU - Hahn, Andreas
AU - Schebb, Nils Helge
N1 - Funding information: The provision of the DHA capsules by DSM Nutritional Products (Columbia, MD, USA) is kindly acknowledged. We would like to thank the participants who contributed their time to this project. This study was supported by grants from the German Research Foundation (Grant SCHE 1801 and SCHU 2516) to NHS and JPS.
PY - 2017/6
Y1 - 2017/6
N2 - INTRODUCTION: EPA and DHA cause different physiological effects, which are in many cases mediated via their oxidative metabolites (oxylipins). However, metabolism studies investigating the effect of either EPA or DHA on comprehensive oxylipin patterns are lacking.MATERIAL AND METHODS: The short and long term (1, 3, 6, and 12 week) effect of 1076mg/d DHA (free of EPA) on free (unesterified) oxylipin concentrations in plasma and lipopolysacharid (LPS) stimulated blood of 12 healthy men (mean age 25.1 ± 1.5 years) was investigated.RESULTS: After DHA supplementation, plasma levels of all DHA-oxylipins (HDHAs, EpDPEs, DiHDPEs) significantly increased (up to 600%) in a time-dependent fashion. Oxylipins of EPA and arachidonic acid (AA) were also affected. Whereas a slight increase in several EPA-derived hydroxy-FAs (including the RvE1 precursor 18-HEPE) and dihydroxy-FAs was observed after DHA supplementation, a trend to a slight decline in AA-derived oxylipin levels was found. In LPS stimulated blood, it is shown that DHA supplementation significantly reduces the ability of immune cells to form AA-derived COX (TXB2 and PGB2) and 12-LOX (12-HETE) eicosanoids. While no increase in EPA COX metabolites was found, n-3 PUFA 12-LOX metabolites of EPA (12-HEPE) and DHA (14-HDHA) were highly induced.CONCLUSION: We demonstrated that DHA supplementation causes a time-dependent shift in the entire oxylipin profile suggesting a cross-linked metabolism of PUFAs and subsequent formation of oxygenated lipid mediators.
AB - INTRODUCTION: EPA and DHA cause different physiological effects, which are in many cases mediated via their oxidative metabolites (oxylipins). However, metabolism studies investigating the effect of either EPA or DHA on comprehensive oxylipin patterns are lacking.MATERIAL AND METHODS: The short and long term (1, 3, 6, and 12 week) effect of 1076mg/d DHA (free of EPA) on free (unesterified) oxylipin concentrations in plasma and lipopolysacharid (LPS) stimulated blood of 12 healthy men (mean age 25.1 ± 1.5 years) was investigated.RESULTS: After DHA supplementation, plasma levels of all DHA-oxylipins (HDHAs, EpDPEs, DiHDPEs) significantly increased (up to 600%) in a time-dependent fashion. Oxylipins of EPA and arachidonic acid (AA) were also affected. Whereas a slight increase in several EPA-derived hydroxy-FAs (including the RvE1 precursor 18-HEPE) and dihydroxy-FAs was observed after DHA supplementation, a trend to a slight decline in AA-derived oxylipin levels was found. In LPS stimulated blood, it is shown that DHA supplementation significantly reduces the ability of immune cells to form AA-derived COX (TXB2 and PGB2) and 12-LOX (12-HETE) eicosanoids. While no increase in EPA COX metabolites was found, n-3 PUFA 12-LOX metabolites of EPA (12-HEPE) and DHA (14-HDHA) were highly induced.CONCLUSION: We demonstrated that DHA supplementation causes a time-dependent shift in the entire oxylipin profile suggesting a cross-linked metabolism of PUFAs and subsequent formation of oxygenated lipid mediators.
KW - 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid/blood
KW - Adult
KW - Arachidonic Acid/metabolism
KW - C-Reactive Protein/metabolism
KW - Dietary Supplements
KW - Docosahexaenoic Acids/administration & dosage
KW - Eicosanoids/metabolism
KW - Fatty Acids, Unsaturated/blood
KW - Germany
KW - Humans
KW - Inflammation/blood
KW - Leukocyte Count
KW - Lipopolysaccharides/toxicity
KW - Male
KW - Oxylipins/blood
KW - Young Adult
KW - Immune cell stimulation
KW - LC n-3 PUFA
KW - EPA
KW - Free oxylipins
KW - DHA
KW - Lipid mediators
UR - http://www.scopus.com/inward/record.url?scp=85021120805&partnerID=8YFLogxK
U2 - 10.1016/j.plefa.2017.06.007
DO - 10.1016/j.plefa.2017.06.007
M3 - Article
C2 - 28651702
VL - 121
SP - 76
EP - 87
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
ER -