Details
| Original language | English |
|---|---|
| Article number | 117924 |
| Journal | Carbohydrate polymers |
| Volume | 262 |
| Early online date | 17 Mar 2021 |
| Publication status | Published - 15 Jun 2021 |
Abstract
In pursuit of a chemically-defined matrix for in vitro cardiac tissue generation, we present dextran (Dex)-derived hydrogels as matrices suitable for bioartificial cardiac tissues (BCT). The dextran hydrogels were generated in situ by using hydrazone formation as the crosslinking reaction. Material properties were flexibly adjusted, by varying the degrees of derivatization and the molecular weight of dextran used. Furthermore, to modulate dextran's bioactivity, cyclic pentapeptide RGD was coupled to its backbone. BCTs were generated by using a blend of modified dextran and human collagen (hColI) in combination with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and fibroblasts. These hColI + Dex blends with or without RGD supported tissue formation and functional maturation of CMs. Contraction forces (hColI + Dex-RGD: 0.27 ± 0.02 mN; hColI + Dex: 0.26 ± 0.01 mN) and frequencies were comparable to published constructs. Thus, we could demonstrate that, independent of the presence of RGD, our covalently linked dextran hydrogels are a promising matrix for building cardiac grafts.
Keywords
- Cardiac tissue engineering, Cardiomyocytes, Dextran, Human induced pluripotent stem cells, Hydrogel, RGD peptide
ASJC Scopus subject areas
- Chemistry(all)
- Organic Chemistry
- Materials Science(all)
- Polymers and Plastics
- Materials Science(all)
- Materials Chemistry
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In: Carbohydrate polymers, Vol. 262, 117924, 15.06.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dextran-based scaffolds for in-situ hydrogelation
T2 - Use for next generation of bioartificial cardiac tissues
AU - Banerjee, Samhita
AU - Szepes, Monika
AU - Dibbert, Nick
AU - Rios-Camacho, Julio-Cesar
AU - Kirschning, Andreas
AU - Gruh, Ina
AU - Dräger, Gerald
N1 - Funding Information: This study was funded by the REBIRTH Cluster of Excellence (EXC62, DFG, Germany) and the REBIRTH Research Center for Translational Regenerative Medicine ( ZN3440 , State of Lower Saxony, Ministry of Science and Culture (Nieders. Vorab), Germany).
PY - 2021/6/15
Y1 - 2021/6/15
N2 - In pursuit of a chemically-defined matrix for in vitro cardiac tissue generation, we present dextran (Dex)-derived hydrogels as matrices suitable for bioartificial cardiac tissues (BCT). The dextran hydrogels were generated in situ by using hydrazone formation as the crosslinking reaction. Material properties were flexibly adjusted, by varying the degrees of derivatization and the molecular weight of dextran used. Furthermore, to modulate dextran's bioactivity, cyclic pentapeptide RGD was coupled to its backbone. BCTs were generated by using a blend of modified dextran and human collagen (hColI) in combination with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and fibroblasts. These hColI + Dex blends with or without RGD supported tissue formation and functional maturation of CMs. Contraction forces (hColI + Dex-RGD: 0.27 ± 0.02 mN; hColI + Dex: 0.26 ± 0.01 mN) and frequencies were comparable to published constructs. Thus, we could demonstrate that, independent of the presence of RGD, our covalently linked dextran hydrogels are a promising matrix for building cardiac grafts.
AB - In pursuit of a chemically-defined matrix for in vitro cardiac tissue generation, we present dextran (Dex)-derived hydrogels as matrices suitable for bioartificial cardiac tissues (BCT). The dextran hydrogels were generated in situ by using hydrazone formation as the crosslinking reaction. Material properties were flexibly adjusted, by varying the degrees of derivatization and the molecular weight of dextran used. Furthermore, to modulate dextran's bioactivity, cyclic pentapeptide RGD was coupled to its backbone. BCTs were generated by using a blend of modified dextran and human collagen (hColI) in combination with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and fibroblasts. These hColI + Dex blends with or without RGD supported tissue formation and functional maturation of CMs. Contraction forces (hColI + Dex-RGD: 0.27 ± 0.02 mN; hColI + Dex: 0.26 ± 0.01 mN) and frequencies were comparable to published constructs. Thus, we could demonstrate that, independent of the presence of RGD, our covalently linked dextran hydrogels are a promising matrix for building cardiac grafts.
KW - Cardiac tissue engineering
KW - Cardiomyocytes
KW - Dextran
KW - Human induced pluripotent stem cells
KW - Hydrogel
KW - RGD peptide
UR - http://www.scopus.com/inward/record.url?scp=85102839813&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2021.117924
DO - 10.1016/j.carbpol.2021.117924
M3 - Article
C2 - 33838803
AN - SCOPUS:85102839813
VL - 262
JO - Carbohydrate polymers
JF - Carbohydrate polymers
SN - 0144-8617
M1 - 117924
ER -