Details
| Original language | English |
|---|---|
| Article number | 103757 |
| Journal | European journal of soil biology |
| Volume | 126 |
| Early online date | 16 Jul 2025 |
| Publication status | Published - Sept 2025 |
Abstract
Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess 13C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.
Keywords
- Metagenomics, Microbial community, Priming effect, Soil organic carbon, Stable isotope probing
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
- Agricultural and Biological Sciences(all)
- Insect Science
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In: European journal of soil biology, Vol. 126, 103757, 09.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Deciphering the microbial mechanisms underlying glucose induced soil priming effects under low and high nutrient levels
AU - Wang, Jiaqi
AU - Zhang, Xichang
AU - Fu, Yingyi
AU - Van Zwieten, Lukas
AU - Sun, Han
AU - Guggenberger, Georg
AU - Hu, Lingfei
AU - Luo, Yu
AU - Ge, Tida
AU - Kuzyakov, Yakov
N1 - Publisher Copyright: © 2025 Elsevier Masson SAS
PY - 2025/9
Y1 - 2025/9
N2 - Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess 13C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.
AB - Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess 13C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.
KW - Metagenomics
KW - Microbial community
KW - Priming effect
KW - Soil organic carbon
KW - Stable isotope probing
UR - http://www.scopus.com/inward/record.url?scp=105010691388&partnerID=8YFLogxK
U2 - 10.1016/j.ejsobi.2025.103757
DO - 10.1016/j.ejsobi.2025.103757
M3 - Article
AN - SCOPUS:105010691388
VL - 126
JO - European journal of soil biology
JF - European journal of soil biology
SN - 1164-5563
M1 - 103757
ER -