Details
| Original language | English |
|---|---|
| Article number | 497 |
| Pages (from-to) | 1-14 |
| Number of pages | 14 |
| Journal | Plants |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 5 Mar 2021 |
Abstract
An intact skin is essential in high-quality apples. Ongoing deposition of cuticular mate-rial during fruit development may decrease microcracking. Our objective was to establish a system for quantifying cutin and wax deposition in developing apple fruit. Oleic acid (13C and14C labelled) and palmitic acid (14C labelled) were fed to developing apples and the amounts incorporated in the cutin and wax fractions were quantified. The incorporation of14C oleic acid (C18) was significantly higher than that of14C palmitic acid (C16) and the incorporation in the cutin fraction exceeded that in the wax fraction. The amount of precursor incorporated in the cutin increased asymptotically with time, but the amount in the wax fraction remained about constant. Increasing the concentration of the precursor applied generally increased incorporation. Incorporation in the cutin fraction was high during early development (43 days after full bloom) and decreased towards maturity. Incorporation was higher from a dilute donor solution (infinite dose feeding) than from a donor solution subjected to drying (finite dose feeding) or from perfusion of the precursor by injection. Feeding the skin of a developing apple with oleic acid resulted in significant incorporation in the cutin fraction under both laboratory and field conditions.
Keywords
- Cuticle, Cutin, Epidermis, Hypodermis, Malus × domestica, Oleic acid, Palmitic acid, Wax
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Environmental Science(all)
- Ecology
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Plants, Vol. 10, No. 3, 497, 05.03.2021, p. 1-14.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cutin synthesis in developing, field-grown apple fruit examined by external feeding of labelled precursors
AU - Si, Yiru
AU - Khanal, Bishnu P.
AU - Sauheitl, Leopold
AU - Knoche, Moritz
N1 - Funding: This research was funded by a grant (KH 374/2-1) from the Deutsche Forschungsge-meinschaft (DFG). The publication of this article was funded by the Open Access fund of the Leibniz Universität Hannover.
PY - 2021/3/5
Y1 - 2021/3/5
N2 - An intact skin is essential in high-quality apples. Ongoing deposition of cuticular mate-rial during fruit development may decrease microcracking. Our objective was to establish a system for quantifying cutin and wax deposition in developing apple fruit. Oleic acid (13C and14C labelled) and palmitic acid (14C labelled) were fed to developing apples and the amounts incorporated in the cutin and wax fractions were quantified. The incorporation of14C oleic acid (C18) was significantly higher than that of14C palmitic acid (C16) and the incorporation in the cutin fraction exceeded that in the wax fraction. The amount of precursor incorporated in the cutin increased asymptotically with time, but the amount in the wax fraction remained about constant. Increasing the concentration of the precursor applied generally increased incorporation. Incorporation in the cutin fraction was high during early development (43 days after full bloom) and decreased towards maturity. Incorporation was higher from a dilute donor solution (infinite dose feeding) than from a donor solution subjected to drying (finite dose feeding) or from perfusion of the precursor by injection. Feeding the skin of a developing apple with oleic acid resulted in significant incorporation in the cutin fraction under both laboratory and field conditions.
AB - An intact skin is essential in high-quality apples. Ongoing deposition of cuticular mate-rial during fruit development may decrease microcracking. Our objective was to establish a system for quantifying cutin and wax deposition in developing apple fruit. Oleic acid (13C and14C labelled) and palmitic acid (14C labelled) were fed to developing apples and the amounts incorporated in the cutin and wax fractions were quantified. The incorporation of14C oleic acid (C18) was significantly higher than that of14C palmitic acid (C16) and the incorporation in the cutin fraction exceeded that in the wax fraction. The amount of precursor incorporated in the cutin increased asymptotically with time, but the amount in the wax fraction remained about constant. Increasing the concentration of the precursor applied generally increased incorporation. Incorporation in the cutin fraction was high during early development (43 days after full bloom) and decreased towards maturity. Incorporation was higher from a dilute donor solution (infinite dose feeding) than from a donor solution subjected to drying (finite dose feeding) or from perfusion of the precursor by injection. Feeding the skin of a developing apple with oleic acid resulted in significant incorporation in the cutin fraction under both laboratory and field conditions.
KW - Cuticle
KW - Cutin
KW - Epidermis
KW - Hypodermis
KW - Malus × domestica
KW - Oleic acid
KW - Palmitic acid
KW - Wax
UR - http://www.scopus.com/inward/record.url?scp=85102053739&partnerID=8YFLogxK
U2 - 10.3390/plants10030497
DO - 10.3390/plants10030497
M3 - Article
AN - SCOPUS:85102053739
VL - 10
SP - 1
EP - 14
JO - Plants
JF - Plants
IS - 3
M1 - 497
ER -