Details
| Translated title of the contribution | Kryokonservierung von dendritischen Zellen für die Krebstherapie |
|---|---|
| Original language | English |
| Article number | 300 |
| Journal | Problems of cryobiology :scientific and theoretical journal |
| Volume | 32 |
| Issue number | 4 |
| Publication status | Published - 30 Dec 2022 |
Abstract
Sustainable Development Goals
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In: Problems of cryobiology :scientific and theoretical journal, Vol. 32, No. 4, 300, 30.12.2022.
Research output: Contribution to journal › Article › Research
}
TY - JOUR
T1 - Cryopreservation of Dendritic Cells for Cancer Therapy
AU - Deeb, Tarek
AU - Zeh, Lena
AU - Rittinghaus, Tim Sören Frederik
AU - Dettmar-Richardt, Claudia
AU - Grammel, Simon
AU - Glasmacher, Birgit
PY - 2022/12/30
Y1 - 2022/12/30
N2 - In recent decades, cell therapy has become increasingly important as a complementary approach to cancer treat-ment. Dendritic cell therapy shows great potential for the treatment of cancer. Better availability of this cell therapy should be ensured by cryopreservation. The project is dedicated to the investigation of a supply chain model in which equine dendritic cells derived from monocytes are first cultured, shipped and cryopreserved. Then, the samples are thawed and shipped back after three specifi c time periods corresponding to the real therapeutic process. In total, the quality, as well as the quantity (automatic cell counting as well as vitality determination) of ൱൰ samples from four diff erent animals, is evaluated before and after the cryopreservation process in terms of storage time, con-centration, and total volume. Here, a composition of ൯ mil-lion cells in ൫ ml seems to be the most suitable for the pro-cedure and provided better results (overall vitality ≥൱൲%). In addition, isotonic saline was found to have the potential to replace autologous plasma in cryoprotectants.
AB - In recent decades, cell therapy has become increasingly important as a complementary approach to cancer treat-ment. Dendritic cell therapy shows great potential for the treatment of cancer. Better availability of this cell therapy should be ensured by cryopreservation. The project is dedicated to the investigation of a supply chain model in which equine dendritic cells derived from monocytes are first cultured, shipped and cryopreserved. Then, the samples are thawed and shipped back after three specifi c time periods corresponding to the real therapeutic process. In total, the quality, as well as the quantity (automatic cell counting as well as vitality determination) of ൱൰ samples from four diff erent animals, is evaluated before and after the cryopreservation process in terms of storage time, con-centration, and total volume. Here, a composition of ൯ mil-lion cells in ൫ ml seems to be the most suitable for the pro-cedure and provided better results (overall vitality ≥൱൲%). In addition, isotonic saline was found to have the potential to replace autologous plasma in cryoprotectants.
U2 - 10.15407/cryo32.04.300a
DO - 10.15407/cryo32.04.300a
M3 - Article
VL - 32
JO - Problems of cryobiology :scientific and theoretical journal
JF - Problems of cryobiology :scientific and theoretical journal
SN - 0233-7673
IS - 4
M1 - 300
ER -