Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli

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Translated title of the contributionWhole-Cell Biocatalytical Production of the Sesquiterpene Presilphiperfolan-8b-ol in Pathway-Engineered Escherichia coli
Original languageGerman
Pages (from-to)576-586
Number of pages11
JournalChemie-Ingenieur-Technik
Volume95
Issue number4
Publication statusPublished - 22 Mar 2023

Abstract

Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.

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Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli. / Wildhagen, Maik; Pudenz, Tabea; Nguyen, Trang et al.
In: Chemie-Ingenieur-Technik, Vol. 95, No. 4, 22.03.2023, p. 576-586.

Research output: Contribution to journalArticleResearchpeer review

Wildhagen, Maik ; Pudenz, Tabea ; Nguyen, Trang et al. / Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli. In: Chemie-Ingenieur-Technik. 2023 ; Vol. 95, No. 4. pp. 576-586.
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title = "Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli",
abstract = "Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.",
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author = "Maik Wildhagen and Tabea Pudenz and Trang Nguyen and Andreas Kirschning and Sascha Beutel",
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AU - Pudenz, Tabea

AU - Nguyen, Trang

AU - Kirschning, Andreas

AU - Beutel, Sascha

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PY - 2023/3/22

Y1 - 2023/3/22

N2 - Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.

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