Details
| Original language | English |
|---|---|
| Pages (from-to) | 1319-1329 |
| Number of pages | 11 |
| Journal | Molecular plant pathology |
| Volume | 24 |
| Issue number | 10 |
| Publication status | Published - 15 Sept 2023 |
Abstract
In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.
Keywords
- beet mosaic virus, betalain biosynthesis, BvMYB1, Caryophyllales, infectious cDNA clone, virus tracking
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Soil Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Molecular plant pathology, Vol. 24, No. 10, 15.09.2023, p. 1319-1329.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris
AU - Rollwage, Lukas
AU - Maiss, Edgar
AU - Menzel, Wulf
AU - Hossain, Roxana
AU - Varrelmann, Mark
N1 - Funding Information: The authors would like to thank all staff of the Institute of Sugar Beet Research, Leibniz University Hannover and Leibniz Institute DSMZ for their technical support on laboratory work.
PY - 2023/9/15
Y1 - 2023/9/15
N2 - In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.
AB - In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.
KW - beet mosaic virus
KW - betalain biosynthesis
KW - BvMYB1
KW - Caryophyllales
KW - infectious cDNA clone
KW - virus tracking
UR - http://www.scopus.com/inward/record.url?scp=85164478280&partnerID=8YFLogxK
U2 - 10.1111/mpp.13372
DO - 10.1111/mpp.13372
M3 - Article
AN - SCOPUS:85164478280
VL - 24
SP - 1319
EP - 1329
JO - Molecular plant pathology
JF - Molecular plant pathology
SN - 1464-6722
IS - 10
ER -