Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Samir AE Bashandy
  • Marawan AElbaset
  • Fatma A.A. Ibrahim
  • Sherif A. Abdelmottaleb Moussa
  • Noha A. Abd El-Latif
  • Sherif M. Afifi
  • Tuba Esatbeyoglu
  • Ahmed M.A. El-Seidy

External Research Organisations

  • National Research Centre (NRC)
  • University of Bologna
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Details

Original languageEnglish
Article number100260
JournalJournal of Trace Elements and Minerals
Volume14
Early online date24 Aug 2025
Publication statusPublished - Dec 2025

Abstract

Background: Obesity and overweight result in low-grade systemic inflammation associated with an increase of oxidative stress and hepcidin discharging and lowering iron absorption giving rise to anemia. This study is aimed to investigate the anti-anemic effect of cerium oxide nanoparticles (CeO 2NPs) in obese rats via investigation of hepcidin expression, ferritin, inflammatory markers, and oxidative stress parameters Methods: CeO 2NPs were synthesized using sol–gel method. Eight male rats were specified as control non-obese. Twenty-four Wistar adult male rats were subjected to high fat diet /sucrose and they were divided into 3 groups; obese, 15 or 30 mg.kg −1 CeO 2NPs IP treatment for the subsequent 8 weeks. Blood cell counts, hepicidin gene expression in adipose tissue, inflammatory and oxidative stress parameters beside iron parameters were evaluated in the experimental groups. Results: CeO 2NPs crystallite size is 48.88 nm. The ratio of Ce 4+ was 69.33 %. The intraperitoneal injection of obese rats with CeO 2NPs substantially reduced the elevetion in WBCs, platelets count, plasma cholesterol, triglycerides, malondialdehyde (MDA), superoxide dismutase (SOD), TNF-α (Tumor necrosis factor alpha), IL-6 (Interleukin-6), and CRP (C-reactive protein) levels. Also, CeO 2NPs combate the increase of hepcidin gene expression, ferritin level, iron content in adipose tissue, body weight, body mass index (BMI) and plasma leptin concentration of obese rats. Moreover, RBCs and WBCs count, Hb level, adiponectin level, and reduced glutathione (GSH) were enhanced in obese rats given CeO 2NPs. Conclusion: CeO 2NPs may have anti-anemic activity in obese rats via management of hepcidin gene expression along with antioxidant and anti-inflammatory properties.

Keywords

    BMI, CeO NPs, Hepcidin gene expression, Iron overload, Obesity, Oxidative stress

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation. / Bashandy, Samir AE; AElbaset, Marawan; Ibrahim, Fatma A.A. et al.
In: Journal of Trace Elements and Minerals, Vol. 14, 100260, 12.2025.

Research output: Contribution to journalArticleResearchpeer review

Bashandy, SAE, AElbaset, M, Ibrahim, FAA, Moussa, SAA, El-Latif, NAA, Afifi, SM, Esatbeyoglu, T & El-Seidy, AMA 2025, 'Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation', Journal of Trace Elements and Minerals, vol. 14, 100260. https://doi.org/10.1016/j.jtemin.2025.100260
Bashandy, S. AE., AElbaset, M., Ibrahim, F. A. A., Moussa, S. A. A., El-Latif, N. A. A., Afifi, S. M., Esatbeyoglu, T., & El-Seidy, A. M. A. (2025). Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation. Journal of Trace Elements and Minerals, 14, Article 100260. https://doi.org/10.1016/j.jtemin.2025.100260
Bashandy SAE, AElbaset M, Ibrahim FAA, Moussa SAA, El-Latif NAA, Afifi SM et al. Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation. Journal of Trace Elements and Minerals. 2025 Dec;14:100260. Epub 2025 Aug 24. doi: 10.1016/j.jtemin.2025.100260
Bashandy, Samir AE ; AElbaset, Marawan ; Ibrahim, Fatma A.A. et al. / Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation. In: Journal of Trace Elements and Minerals. 2025 ; Vol. 14.
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title = "Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation",
abstract = "Background: Obesity and overweight result in low-grade systemic inflammation associated with an increase of oxidative stress and hepcidin discharging and lowering iron absorption giving rise to anemia. This study is aimed to investigate the anti-anemic effect of cerium oxide nanoparticles (CeO 2NPs) in obese rats via investigation of hepcidin expression, ferritin, inflammatory markers, and oxidative stress parameters Methods: CeO 2NPs were synthesized using sol–gel method. Eight male rats were specified as control non-obese. Twenty-four Wistar adult male rats were subjected to high fat diet /sucrose and they were divided into 3 groups; obese, 15 or 30 mg.kg −1 CeO 2NPs IP treatment for the subsequent 8 weeks. Blood cell counts, hepicidin gene expression in adipose tissue, inflammatory and oxidative stress parameters beside iron parameters were evaluated in the experimental groups. Results: CeO 2NPs crystallite size is 48.88 nm. The ratio of Ce 4+ was 69.33 %. The intraperitoneal injection of obese rats with CeO 2NPs substantially reduced the elevetion in WBCs, platelets count, plasma cholesterol, triglycerides, malondialdehyde (MDA), superoxide dismutase (SOD), TNF-α (Tumor necrosis factor alpha), IL-6 (Interleukin-6), and CRP (C-reactive protein) levels. Also, CeO 2NPs combate the increase of hepcidin gene expression, ferritin level, iron content in adipose tissue, body weight, body mass index (BMI) and plasma leptin concentration of obese rats. Moreover, RBCs and WBCs count, Hb level, adiponectin level, and reduced glutathione (GSH) were enhanced in obese rats given CeO 2NPs. Conclusion: CeO 2NPs may have anti-anemic activity in obese rats via management of hepcidin gene expression along with antioxidant and anti-inflammatory properties.",
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TY - JOUR

T1 - Anti-Anemic Potential of Cerium Oxide Nanoparticles in Diet-Induced Obese Rats: Role of Hepcidin, Oxidative Stress, and Inflammation

AU - Bashandy, Samir AE

AU - AElbaset, Marawan

AU - Ibrahim, Fatma A.A.

AU - Moussa, Sherif A. Abdelmottaleb

AU - El-Latif, Noha A. Abd

AU - Afifi, Sherif M.

AU - Esatbeyoglu, Tuba

AU - El-Seidy, Ahmed M.A.

N1 - Publisher Copyright: © 2025 The Author(s)

PY - 2025/12

Y1 - 2025/12

N2 - Background: Obesity and overweight result in low-grade systemic inflammation associated with an increase of oxidative stress and hepcidin discharging and lowering iron absorption giving rise to anemia. This study is aimed to investigate the anti-anemic effect of cerium oxide nanoparticles (CeO 2NPs) in obese rats via investigation of hepcidin expression, ferritin, inflammatory markers, and oxidative stress parameters Methods: CeO 2NPs were synthesized using sol–gel method. Eight male rats were specified as control non-obese. Twenty-four Wistar adult male rats were subjected to high fat diet /sucrose and they were divided into 3 groups; obese, 15 or 30 mg.kg −1 CeO 2NPs IP treatment for the subsequent 8 weeks. Blood cell counts, hepicidin gene expression in adipose tissue, inflammatory and oxidative stress parameters beside iron parameters were evaluated in the experimental groups. Results: CeO 2NPs crystallite size is 48.88 nm. The ratio of Ce 4+ was 69.33 %. The intraperitoneal injection of obese rats with CeO 2NPs substantially reduced the elevetion in WBCs, platelets count, plasma cholesterol, triglycerides, malondialdehyde (MDA), superoxide dismutase (SOD), TNF-α (Tumor necrosis factor alpha), IL-6 (Interleukin-6), and CRP (C-reactive protein) levels. Also, CeO 2NPs combate the increase of hepcidin gene expression, ferritin level, iron content in adipose tissue, body weight, body mass index (BMI) and plasma leptin concentration of obese rats. Moreover, RBCs and WBCs count, Hb level, adiponectin level, and reduced glutathione (GSH) were enhanced in obese rats given CeO 2NPs. Conclusion: CeO 2NPs may have anti-anemic activity in obese rats via management of hepcidin gene expression along with antioxidant and anti-inflammatory properties.

AB - Background: Obesity and overweight result in low-grade systemic inflammation associated with an increase of oxidative stress and hepcidin discharging and lowering iron absorption giving rise to anemia. This study is aimed to investigate the anti-anemic effect of cerium oxide nanoparticles (CeO 2NPs) in obese rats via investigation of hepcidin expression, ferritin, inflammatory markers, and oxidative stress parameters Methods: CeO 2NPs were synthesized using sol–gel method. Eight male rats were specified as control non-obese. Twenty-four Wistar adult male rats were subjected to high fat diet /sucrose and they were divided into 3 groups; obese, 15 or 30 mg.kg −1 CeO 2NPs IP treatment for the subsequent 8 weeks. Blood cell counts, hepicidin gene expression in adipose tissue, inflammatory and oxidative stress parameters beside iron parameters were evaluated in the experimental groups. Results: CeO 2NPs crystallite size is 48.88 nm. The ratio of Ce 4+ was 69.33 %. The intraperitoneal injection of obese rats with CeO 2NPs substantially reduced the elevetion in WBCs, platelets count, plasma cholesterol, triglycerides, malondialdehyde (MDA), superoxide dismutase (SOD), TNF-α (Tumor necrosis factor alpha), IL-6 (Interleukin-6), and CRP (C-reactive protein) levels. Also, CeO 2NPs combate the increase of hepcidin gene expression, ferritin level, iron content in adipose tissue, body weight, body mass index (BMI) and plasma leptin concentration of obese rats. Moreover, RBCs and WBCs count, Hb level, adiponectin level, and reduced glutathione (GSH) were enhanced in obese rats given CeO 2NPs. Conclusion: CeO 2NPs may have anti-anemic activity in obese rats via management of hepcidin gene expression along with antioxidant and anti-inflammatory properties.

KW - BMI

KW - CeO NPs

KW - Hepcidin gene expression

KW - Iron overload

KW - Obesity

KW - Oxidative stress

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U2 - 10.1016/j.jtemin.2025.100260

DO - 10.1016/j.jtemin.2025.100260

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JO - Journal of Trace Elements and Minerals

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