Details
| Original language | English |
|---|---|
| Pages (from-to) | 84-100 |
| Number of pages | 17 |
| Journal | Environmental microbiology |
| Volume | 16 |
| Issue number | 1 |
| Publication status | Published - Jan 2014 |
Abstract
2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
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In: Environmental microbiology, Vol. 16, No. 1, 01.2014, p. 84-100.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Agricultural soil and drilosphere as reservoirs of new and unusual assimilators of 2,4-dichlorophenol carbon
AU - Dallinger, Anja
AU - Horn, Marcus A.
N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/1
Y1 - 2014/1
N2 - 2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.
AB - 2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.
UR - http://www.scopus.com/inward/record.url?scp=84891623985&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.12209
DO - 10.1111/1462-2920.12209
M3 - Article
VL - 16
SP - 84
EP - 100
JO - Environmental microbiology
JF - Environmental microbiology
SN - 1462-2912
IS - 1
ER -