Details
| Original language | English |
|---|---|
| Pages (from-to) | 1864-1871 |
| Number of pages | 8 |
| Journal | Arthritis and Rheumatology |
| Volume | 66 |
| Issue number | 7 |
| Early online date | 18 Mar 2014 |
| Publication status | Published - Jul 2014 |
| Externally published | Yes |
Abstract
Objective To investigate the role of adhesion molecules in C protein-induced myositis (CIM), a murine model of polymyositis (PM). Methods CIM was induced in wild-type mice, L-selectin-deficient (L-selectin-/-) mice, intercellular adhesion molecule 1 (ICAM-1)-deficient (ICAM-1 -/-) mice, and mice deficient in both L-selectin and ICAM-1 (L-selectin-/-ICAM-1-/- mice). Myositis severity, inflammatory cell infiltration, and messenger RNA expression in the inflamed muscles were analyzed. The effect of dendritic polyglycerol sulfate, a synthetic inhibitor that suppresses the function of L-selectin and endothelial P-selectin, was also examined. Results L-selectin-/- mice and L-selectin-/-ICAM-1-/- mice developed significantly less severe myositis compared to wild-type mice, while ICAM-1 deficiency did not inhibit the development of myositis. L-selectin-/- mice that received wild-type T cells developed myositis. Treatment with dendritic polyglycerol sulfate significantly diminished the severity of myositis in wild-type mice compared to treatment with control. Conclusion These data indicate that L-selectin plays a major role in the development of CIM, whereas ICAM-1 plays a lesser role, if any, in the development of CIM. L-selectin-targeted therapy may be a candidate for the treatment of PM.
ASJC Scopus subject areas
- Medicine(all)
- Immunology and Allergy
- Medicine(all)
- Rheumatology
- Immunology and Microbiology(all)
- Immunology
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In: Arthritis and Rheumatology, Vol. 66, No. 7, 07.2014, p. 1864-1871.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A crucial role of L-selectin in C protein
T2 - Induced experimental polymyositis in mice
AU - Oishi, Kyosuke
AU - Hamaguchi, Yasuhito
AU - Matsushita, Takashi
AU - Hasegawa, Minoru
AU - Okiyama, Naoko
AU - Dernedde, Jens
AU - Weinhart, Marie
AU - Haag, Rainer
AU - Tedder, Thomas F.
AU - Takehara, Kazuhiko
AU - Kohsaka, Hitoshi
AU - Fujimoto, Manabu
PY - 2014/7
Y1 - 2014/7
N2 - Objective To investigate the role of adhesion molecules in C protein-induced myositis (CIM), a murine model of polymyositis (PM). Methods CIM was induced in wild-type mice, L-selectin-deficient (L-selectin-/-) mice, intercellular adhesion molecule 1 (ICAM-1)-deficient (ICAM-1 -/-) mice, and mice deficient in both L-selectin and ICAM-1 (L-selectin-/-ICAM-1-/- mice). Myositis severity, inflammatory cell infiltration, and messenger RNA expression in the inflamed muscles were analyzed. The effect of dendritic polyglycerol sulfate, a synthetic inhibitor that suppresses the function of L-selectin and endothelial P-selectin, was also examined. Results L-selectin-/- mice and L-selectin-/-ICAM-1-/- mice developed significantly less severe myositis compared to wild-type mice, while ICAM-1 deficiency did not inhibit the development of myositis. L-selectin-/- mice that received wild-type T cells developed myositis. Treatment with dendritic polyglycerol sulfate significantly diminished the severity of myositis in wild-type mice compared to treatment with control. Conclusion These data indicate that L-selectin plays a major role in the development of CIM, whereas ICAM-1 plays a lesser role, if any, in the development of CIM. L-selectin-targeted therapy may be a candidate for the treatment of PM.
AB - Objective To investigate the role of adhesion molecules in C protein-induced myositis (CIM), a murine model of polymyositis (PM). Methods CIM was induced in wild-type mice, L-selectin-deficient (L-selectin-/-) mice, intercellular adhesion molecule 1 (ICAM-1)-deficient (ICAM-1 -/-) mice, and mice deficient in both L-selectin and ICAM-1 (L-selectin-/-ICAM-1-/- mice). Myositis severity, inflammatory cell infiltration, and messenger RNA expression in the inflamed muscles were analyzed. The effect of dendritic polyglycerol sulfate, a synthetic inhibitor that suppresses the function of L-selectin and endothelial P-selectin, was also examined. Results L-selectin-/- mice and L-selectin-/-ICAM-1-/- mice developed significantly less severe myositis compared to wild-type mice, while ICAM-1 deficiency did not inhibit the development of myositis. L-selectin-/- mice that received wild-type T cells developed myositis. Treatment with dendritic polyglycerol sulfate significantly diminished the severity of myositis in wild-type mice compared to treatment with control. Conclusion These data indicate that L-selectin plays a major role in the development of CIM, whereas ICAM-1 plays a lesser role, if any, in the development of CIM. L-selectin-targeted therapy may be a candidate for the treatment of PM.
UR - http://www.scopus.com/inward/record.url?scp=84903479676&partnerID=8YFLogxK
U2 - 10.1002/art.38630
DO - 10.1002/art.38630
M3 - Article
C2 - 24644046
AN - SCOPUS:84903479676
VL - 66
SP - 1864
EP - 1871
JO - Arthritis and Rheumatology
JF - Arthritis and Rheumatology
SN - 2326-5191
IS - 7
ER -