3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells

Research output: Contribution to journal › Article › Research › peer review

Authors

  • Vitalii V. Mutsenko
  • Vasilii V. Bazhenov
  • Olena Rogulska
  • Dmitriy N. Tarusin
  • Kathleen Schütz
  • Sophie Brüggemeier
  • Elke Gossla
  • Ashwini R. Akkineni
  • Heike Meißner
  • Anja Lode
  • Stephan Meschke
  • Andre Ehrlich
  • Slavica Petović
  • Rajko Martinović
  • Mirko Djurović
  • Allison L. Stelling
  • Sergey Nikulin
  • Sergey Rodin
  • Alexander Tonevitsky
  • Michael Gelinsky
  • Alexander Y. Petrenko
  • Birgit Glasmacher
  • Hermann Ehrlich

Research Organisations

External Research Organisations

  • TU Bergakademie Freiberg - University of Resources
  • Technische Universität Dresden
  • BromMarin GmbH
  • University of Montenegro
  • Duke University
  • Moscow Institute of Physics and Technology
  • P. Hertsen Moscow Oncology Research Institute
  • National Academy of Sciences of Ukraine
  • Institute for Problems of Cryobiology and Cryomedicine
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Details

Original languageEnglish
Pages (from-to)1966-1974
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume104
Early online date25 Mar 2017
Publication statusPublished - Nov 2017

Abstract

The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

Keywords

    Chitin scaffolds, Mesenchymal stromal cells, Sponges

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. / Mutsenko, Vitalii V.; Bazhenov, Vasilii V.; Rogulska, Olena et al.
In: International Journal of Biological Macromolecules, Vol. 104, 11.2017, p. 1966-1974.

Research output: Contribution to journal › Article › Research › peer review

Mutsenko, VV, Bazhenov, VV, Rogulska, O, Tarusin, DN, Schütz, K, Brüggemeier, S, Gossla, E, Akkineni, AR, Meißner, H, Lode, A, Meschke, S, Ehrlich, A, Petović, S, Martinović, R, Djurović, M, Stelling, AL, Nikulin, S, Rodin, S, Tonevitsky, A, Gelinsky, M, Petrenko, AY, Glasmacher, B & Ehrlich, H 2017, '3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells', International Journal of Biological Macromolecules, vol. 104, pp. 1966-1974. https://doi.org/10.1016/j.ijbiomac.2017.03.116
Mutsenko, V. V., Bazhenov, V. V., Rogulska, O., Tarusin, D. N., Schütz, K., Brüggemeier, S., Gossla, E., Akkineni, A. R., Meißner, H., Lode, A., Meschke, S., Ehrlich, A., Petović, S., Martinović, R., Djurović, M., Stelling, A. L., Nikulin, S., Rodin, S., Tonevitsky, A., ... Ehrlich, H. (2017). 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. International Journal of Biological Macromolecules, 104, 1966-1974. https://doi.org/10.1016/j.ijbiomac.2017.03.116
Mutsenko VV, Bazhenov VV, Rogulska O, Tarusin DN, Schütz K, Brüggemeier S et al. 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. International Journal of Biological Macromolecules. 2017 Nov;104:1966-1974. Epub 2017 Mar 25. doi: 10.1016/j.ijbiomac.2017.03.116
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abstract = "The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.",
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AU - Mutsenko, Vitalii V.

AU - Bazhenov, Vasilii V.

AU - Rogulska, Olena

AU - Tarusin, Dmitriy N.

AU - Schütz, Kathleen

AU - Brüggemeier, Sophie

AU - Gossla, Elke

AU - Akkineni, Ashwini R.

AU - Meißner, Heike

AU - Lode, Anja

AU - Meschke, Stephan

AU - Ehrlich, Andre

AU - Petović, Slavica

AU - Martinović, Rajko

AU - Djurović, Mirko

AU - Stelling, Allison L.

AU - Nikulin, Sergey

AU - Rodin, Sergey

AU - Tonevitsky, Alexander

AU - Gelinsky, Michael

AU - Petrenko, Alexander Y.

AU - Glasmacher, Birgit

AU - Ehrlich, Hermann

N1 - Funding information: The present work was partially supported by Leonhard-Euler-Programm from German Academic Exchange Service (DAAD, Germany) as well as DFG Grant EH 394-3 (DFG, Germany). The authors would like to thank Igor Kovalenko, Galina Bozhok, Vitaliy Kholodny and Ortrud Zieschang for excellent technical assistance.

PY - 2017/11

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