Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Lothar Lauterboeck
  • Debapriya Saha
  • Anamika Chatterjee
  • Nicola Hofmann
  • Birgit Glasmacher

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Details

OriginalspracheEnglisch
Seiten (von - bis)530-538
Seitenumfang9
FachzeitschriftBiopreservation and biobanking
Jahrgang14
Ausgabenummer6
Frühes Online-Datum7 Sept. 2016
PublikationsstatusVeröffentlicht - 1 Dez. 2016

Abstract

In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks. This gives rise to a need for well-characterized and serum-free freezing media for application in cryopreservation. For this purpose, cryobiological additives such as methylcellulose, poloxamer-188, and α-tocopherol, which have previously been shown to exhibit a cytoprotective activity, have been investigated for cryoprotection on stem cells. With this strategy, the application of fetal bovine serum (FBS) could be avoided and the concentration of toxic cryoprotective agents such as dimethyl sulfoxide (DMSO) could be reduced. Our results suggest that the viability, as well as the adipogenic and osteogenic differentiation capacity of the thawed bone marrow-derived multipotent stromal stem cells, could be maintained using a freezing medium without FBS consisting of methylcellulose, poloxamer, and α-tocopherol with only 2.5% DMSO (% v/v).

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Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus. / Lauterboeck, Lothar; Saha, Debapriya; Chatterjee, Anamika et al.
in: Biopreservation and biobanking, Jahrgang 14, Nr. 6, 01.12.2016, S. 530-538.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Lauterboeck L, Saha D, Chatterjee A, Hofmann N, Glasmacher B. Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus. Biopreservation and biobanking. 2016 Dez 1;14(6):530-538. Epub 2016 Sep 7. doi: 10.1089/bio.2016.0038
Lauterboeck, Lothar ; Saha, Debapriya ; Chatterjee, Anamika et al. / Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus. in: Biopreservation and biobanking. 2016 ; Jahrgang 14, Nr. 6. S. 530-538.
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