TY - JOUR

T1 - Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening

T2 - Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

AU - Bündig, Christin

AU - Vu, T. H.

AU - Meise, P.

AU - Seddig, Sylvia

AU - Schum, A.

AU - Winkelmann, Traud

N1 - Funding Information: The study was conducted with funding provided by the Federal Ministry of Food and Agriculture (BMEL) through the Agency of Renewable Resources (FNR), funding no.: 22023511 (LUH) and 22023311 (JKI). The authors would like to thank Dr Klaus Dehmer for in?vitro material of S.?chacoense and S.?tarijense. Publisher Copyright: © 2016 Blackwell Verlag GmbH Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/6

Y1 - 2017/6

N2 - Solanum tuberosum (potato) as a drought sensitive plant is also one of the most promising plants to meet the demands for food and starch of a growing population. Distinguishing genotypes into tolerant and susceptible is therefore of utmost interest. We subjected eighteen potato genotypes and two wild species, S. tarijense and S. chacoense, to osmotic stress applied in vitro by addition of 0.2 m sorbitol to a solid medium. Here, we report that a ratio of root:shoot dry mass (DM) together with the SSI (stress susceptibility index, equivalent to drought susceptibility index by Fischer and Maurer, Aust. J. Agron. Res., 29, 1978) of shoot DM were found to be relevant parameters to characterize genotypes in vitro for their osmotic stress tolerance. Drought stress data from pot trials in a rainout shelter (2013 and 2015) correlated poorly with the data obtained in in vitro experiments. However, the most tolerant and most sensitive genotypes in vitro were also categorized to be more tolerant or sensitive than the average to drought stress in vivo. Both, under in vitro and in vivo conditions, proline displayed an increase under osmotic stress conditions in nearly all potatoes tested, but no direct correlations were found to stress tolerance. However, a genotype classified as tolerant displayed earlier proline accumulation. Proline is thought of as one factor for plants to withstand stressful conditions, but cannot be used to distinguish potato genotypes for their stress tolerance to osmotic stress in vitro. Analysis of the osmotic potential of in vitro and in vivo stressed plants displayed a general increase compared to the control.

AB - Solanum tuberosum (potato) as a drought sensitive plant is also one of the most promising plants to meet the demands for food and starch of a growing population. Distinguishing genotypes into tolerant and susceptible is therefore of utmost interest. We subjected eighteen potato genotypes and two wild species, S. tarijense and S. chacoense, to osmotic stress applied in vitro by addition of 0.2 m sorbitol to a solid medium. Here, we report that a ratio of root:shoot dry mass (DM) together with the SSI (stress susceptibility index, equivalent to drought susceptibility index by Fischer and Maurer, Aust. J. Agron. Res., 29, 1978) of shoot DM were found to be relevant parameters to characterize genotypes in vitro for their osmotic stress tolerance. Drought stress data from pot trials in a rainout shelter (2013 and 2015) correlated poorly with the data obtained in in vitro experiments. However, the most tolerant and most sensitive genotypes in vitro were also categorized to be more tolerant or sensitive than the average to drought stress in vivo. Both, under in vitro and in vivo conditions, proline displayed an increase under osmotic stress conditions in nearly all potatoes tested, but no direct correlations were found to stress tolerance. However, a genotype classified as tolerant displayed earlier proline accumulation. Proline is thought of as one factor for plants to withstand stressful conditions, but cannot be used to distinguish potato genotypes for their stress tolerance to osmotic stress in vitro. Analysis of the osmotic potential of in vitro and in vivo stressed plants displayed a general increase compared to the control.

KW - in vitro

KW - osmotic stress

KW - Solanum tuberosum

UR - http://www.scopus.com/inward/record.url?scp=84983395793&partnerID=8YFLogxK

U2 - 10.1111/jac.12186

DO - 10.1111/jac.12186

M3 - Article

AN - SCOPUS:84983395793

VL - 203

SP - 206

EP - 218

JO - Journal of Agronomy and Crop Science

JF - Journal of Agronomy and Crop Science

SN - 0931-2250

IS - 3

ER -