Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 207-215 |
| Seitenumfang | 9 |
| Fachzeitschrift | Propagation of Ornamental Plants |
| Jahrgang | 9 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 2009 |
Abstract
Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Forstwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Propagation of Ornamental Plants, Jahrgang 9, Nr. 4, 2009, S. 207-215.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Use of the PMI/mannose selection system for genetic transformation of Kalanchoe blossfeldiana poelln
AU - Ilczuk, Agnieszka
AU - Mibus, Heiko
AU - Schmidt, Malthe
AU - Serek, Margrethe
PY - 2009
Y1 - 2009
N2 - Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
AB - Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
KW - Agrobacterium tumefaciens
KW - Phosphomannose isomerase
KW - Selective agent
UR - http://www.scopus.com/inward/record.url?scp=73549104600&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:73549104600
VL - 9
SP - 207
EP - 215
JO - Propagation of Ornamental Plants
JF - Propagation of Ornamental Plants
SN - 1311-9109
IS - 4
ER -