Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 1034 |
| Fachzeitschrift | Journal of Fungi |
| Jahrgang | 7 |
| Ausgabenummer | 12 |
| Publikationsstatus | Veröffentlicht - 1 Dez. 2021 |
Abstract
The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Ökologie, Evolution, Verhaltenswissenschaften und Systematik
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
- Medizin (insg.)
- Mikrobiologie (medizinisch)
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in: Journal of Fungi, Jahrgang 7, Nr. 12, 1034, 01.12.2021.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Trichoderma reesei contains a biosynthetic gene cluster that encodes the antifungal agent ilicicolin h
AU - Shenouda, Mary L.
AU - Ambilika, Maria
AU - Cox, Russell J.
N1 - Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG [CO 1328/2-1, INST 187/621-1, INST 187/686-1)]. The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.
AB - The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.
KW - Heterologous expression
KW - Ilicicolin H
KW - PKS-NRPS
KW - Shunt metabolite
KW - Trichoderma reesei
UR - http://www.scopus.com/inward/record.url?scp=85121600351&partnerID=8YFLogxK
U2 - 10.3390/jof7121034
DO - 10.3390/jof7121034
M3 - Article
AN - SCOPUS:85121600351
VL - 7
JO - Journal of Fungi
JF - Journal of Fungi
IS - 12
M1 - 1034
ER -