The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Sebastian Schramm
  • Wilfried Rozhon
  • Adebimpe N. Adedeji-Badmus
  • Yuanyuan Liang
  • Shahran Nayem
  • Traud Winkelmann
  • Brigitte Poppenberger

Externe Organisationen

  • Technische Universität München (TUM)
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Details

OriginalspracheEnglisch
Aufsatznummer702985
FachzeitschriftFrontiers in Plant Science
Jahrgang12
PublikationsstatusVeröffentlicht - 28 Juli 2021

Abstract

Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the plant is still mainly collected from the wild and therefore efforts are made to promote its domestication. However, in addition to beneficial properties, there was first evidence that C. crepidioides can accumulate the highly toxic pyrrolizidine alkaloid (PA) jacobine and here it was investigated, how jacobine production is controlled. Using ecotypes from Africa and Asia that were characterized in terms of their PA profiles, it is shown that the tetraploid C. crepidioides forms jacobine, an ability that its diploid close relative Crassocephalum rubens appears to lack. Evidence is provided that nitrogen (N) deficiency strongly increases jacobine in the leaves of C. crepidioides, that this capacity depends more strongly on the shoot than the root system, and that homospermidine synthase (HSS) activity is not rate-limiting for this reaction. A characterization of HSS gene representation and transcription showed that C. crepidioides and C. rubens possess two functional versions, one of which is conserved, that the HSS transcript is mainly present in roots and that its abundance is not controlled by N deficiency. In summary, this work improves our understanding of how environmental cues impact PA biosynthesis in plants and provides a basis for the development of PA-free C. crepidioides cultivars, which will aid its domestication and safe use.

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The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation. / Schramm, Sebastian; Rozhon, Wilfried; Adedeji-Badmus, Adebimpe N. et al.
in: Frontiers in Plant Science, Jahrgang 12, 702985, 28.07.2021.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Schramm S, Rozhon W, Adedeji-Badmus AN, Liang Y, Nayem S, Winkelmann T et al. The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation. Frontiers in Plant Science. 2021 Jul 28;12:702985. doi: 10.3389/fpls.2021.702985
Schramm, Sebastian ; Rozhon, Wilfried ; Adedeji-Badmus, Adebimpe N. et al. / The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation. in: Frontiers in Plant Science. 2021 ; Jahrgang 12.
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title = "The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation",
abstract = "Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the plant is still mainly collected from the wild and therefore efforts are made to promote its domestication. However, in addition to beneficial properties, there was first evidence that C. crepidioides can accumulate the highly toxic pyrrolizidine alkaloid (PA) jacobine and here it was investigated, how jacobine production is controlled. Using ecotypes from Africa and Asia that were characterized in terms of their PA profiles, it is shown that the tetraploid C. crepidioides forms jacobine, an ability that its diploid close relative Crassocephalum rubens appears to lack. Evidence is provided that nitrogen (N) deficiency strongly increases jacobine in the leaves of C. crepidioides, that this capacity depends more strongly on the shoot than the root system, and that homospermidine synthase (HSS) activity is not rate-limiting for this reaction. A characterization of HSS gene representation and transcription showed that C. crepidioides and C. rubens possess two functional versions, one of which is conserved, that the HSS transcript is mainly present in roots and that its abundance is not controlled by N deficiency. In summary, this work improves our understanding of how environmental cues impact PA biosynthesis in plants and provides a basis for the development of PA-free C. crepidioides cultivars, which will aid its domestication and safe use.",
keywords = "alkaloids, domestication, ebolo, fireweed, genome, neglected crop, nutrients, Yoruba bologi",
author = "Sebastian Schramm and Wilfried Rozhon and Adedeji-Badmus, {Adebimpe N.} and Yuanyuan Liang and Shahran Nayem and Traud Winkelmann and Brigitte Poppenberger",
note = "Funding Information: This work was supported by the Deutsche Akademische Austauschdienst (DAAD Ph.D. fellowship to AA-B). SS and AA-B were members of the TUM Graduate School. Funding Information: We thank the Millenium Seed Bank at Kew Royal Botanic Gardens for seeds of the Crassocephalum accessions used and the IPK gene bank for seeds of the Pisum sativum reference standard. Irene Ziegler and Nicolai K?hler are thanked for technical assistance. Funding. This work was supported by the Deutsche Akademische Austauschdienst (DAAD Ph.D. fellowship to AA-B). SS and AA-B were members of the TUM Graduate School. ",
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Download

TY - JOUR

T1 - The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

AU - Schramm, Sebastian

AU - Rozhon, Wilfried

AU - Adedeji-Badmus, Adebimpe N.

AU - Liang, Yuanyuan

AU - Nayem, Shahran

AU - Winkelmann, Traud

AU - Poppenberger, Brigitte

N1 - Funding Information: This work was supported by the Deutsche Akademische Austauschdienst (DAAD Ph.D. fellowship to AA-B). SS and AA-B were members of the TUM Graduate School. Funding Information: We thank the Millenium Seed Bank at Kew Royal Botanic Gardens for seeds of the Crassocephalum accessions used and the IPK gene bank for seeds of the Pisum sativum reference standard. Irene Ziegler and Nicolai K?hler are thanked for technical assistance. Funding. This work was supported by the Deutsche Akademische Austauschdienst (DAAD Ph.D. fellowship to AA-B). SS and AA-B were members of the TUM Graduate School.

PY - 2021/7/28

Y1 - 2021/7/28

N2 - Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the plant is still mainly collected from the wild and therefore efforts are made to promote its domestication. However, in addition to beneficial properties, there was first evidence that C. crepidioides can accumulate the highly toxic pyrrolizidine alkaloid (PA) jacobine and here it was investigated, how jacobine production is controlled. Using ecotypes from Africa and Asia that were characterized in terms of their PA profiles, it is shown that the tetraploid C. crepidioides forms jacobine, an ability that its diploid close relative Crassocephalum rubens appears to lack. Evidence is provided that nitrogen (N) deficiency strongly increases jacobine in the leaves of C. crepidioides, that this capacity depends more strongly on the shoot than the root system, and that homospermidine synthase (HSS) activity is not rate-limiting for this reaction. A characterization of HSS gene representation and transcription showed that C. crepidioides and C. rubens possess two functional versions, one of which is conserved, that the HSS transcript is mainly present in roots and that its abundance is not controlled by N deficiency. In summary, this work improves our understanding of how environmental cues impact PA biosynthesis in plants and provides a basis for the development of PA-free C. crepidioides cultivars, which will aid its domestication and safe use.

AB - Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the plant is still mainly collected from the wild and therefore efforts are made to promote its domestication. However, in addition to beneficial properties, there was first evidence that C. crepidioides can accumulate the highly toxic pyrrolizidine alkaloid (PA) jacobine and here it was investigated, how jacobine production is controlled. Using ecotypes from Africa and Asia that were characterized in terms of their PA profiles, it is shown that the tetraploid C. crepidioides forms jacobine, an ability that its diploid close relative Crassocephalum rubens appears to lack. Evidence is provided that nitrogen (N) deficiency strongly increases jacobine in the leaves of C. crepidioides, that this capacity depends more strongly on the shoot than the root system, and that homospermidine synthase (HSS) activity is not rate-limiting for this reaction. A characterization of HSS gene representation and transcription showed that C. crepidioides and C. rubens possess two functional versions, one of which is conserved, that the HSS transcript is mainly present in roots and that its abundance is not controlled by N deficiency. In summary, this work improves our understanding of how environmental cues impact PA biosynthesis in plants and provides a basis for the development of PA-free C. crepidioides cultivars, which will aid its domestication and safe use.

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KW - domestication

KW - ebolo

KW - fireweed

KW - genome

KW - neglected crop

KW - nutrients

KW - Yoruba bologi

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U2 - 10.3389/fpls.2021.702985

DO - 10.3389/fpls.2021.702985

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JO - Frontiers in Plant Science

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SN - 1664-462X

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