The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Rachel A Kettles
  • Natalia Tschowri
  • Kevin J Lyons
  • Prateek Sharma
  • Regine Hengge
  • Mark A Webber
  • David C Grainger

Externe Organisationen

  • University of Birmingham
  • Humboldt-Universität zu Berlin (HU Berlin)
  • Quadram Institute
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Details

OriginalspracheEnglisch
Seiten (von - bis)1609-1625
Seitenumfang17
FachzeitschriftMolecular microbiology
Jahrgang112
Ausgabenummer5
Frühes Online-Datum8 Nov. 2019
PublikationsstatusVeröffentlicht - Nov. 2019
Extern publiziertJa

Abstract

The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibiotic resistance. The operon exerts its effect via an encoded transcription factor called MarA that modulates efflux pump and porin expression. In this work, we show that MarA is also a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon. The operon, known to influence the formation of curli fibres and colanic acid, is usually expressed during periods of starvation. Hence, the ycgZ-ymgABC promoter is recognised by σ38 (RpoS)-associated RNA polymerase (RNAP). Surprisingly, MarA does not influence σ38 -dependent transcription. Instead, MarA drives transcription by the housekeeping σ70 -associated RNAP. The effects of MarA on ycgZ-ymgABC expression are coupled with biofilm formation by the rcsCDB phosphorelay system, with YcgZ, YmgA and YmgB forming a complex that directly interacts with the histidine kinase domain of RcsC.

ASJC Scopus Sachgebiete

Zitieren

The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. / Kettles, Rachel A; Tschowri, Natalia; Lyons, Kevin J et al.
in: Molecular microbiology, Jahrgang 112, Nr. 5, 11.2019, S. 1609-1625.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Kettles, RA, Tschowri, N, Lyons, KJ, Sharma, P, Hengge, R, Webber, MA & Grainger, DC 2019, 'The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation', Molecular microbiology, Jg. 112, Nr. 5, S. 1609-1625. https://doi.org/10.1111/mmi.14386
Kettles, R. A., Tschowri, N., Lyons, K. J., Sharma, P., Hengge, R., Webber, M. A., & Grainger, D. C. (2019). The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. Molecular microbiology, 112(5), 1609-1625. https://doi.org/10.1111/mmi.14386
Kettles RA, Tschowri N, Lyons KJ, Sharma P, Hengge R, Webber MA et al. The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. Molecular microbiology. 2019 Nov;112(5):1609-1625. Epub 2019 Nov 8. doi: 10.1111/mmi.14386
Kettles, Rachel A ; Tschowri, Natalia ; Lyons, Kevin J et al. / The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. in: Molecular microbiology. 2019 ; Jahrgang 112, Nr. 5. S. 1609-1625.
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title = "The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation",
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T1 - The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation

AU - Kettles, Rachel A

AU - Tschowri, Natalia

AU - Lyons, Kevin J

AU - Sharma, Prateek

AU - Hengge, Regine

AU - Webber, Mark A

AU - Grainger, David C

N1 - Publisher Copyright: © 2019 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/11

Y1 - 2019/11

N2 - The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibiotic resistance. The operon exerts its effect via an encoded transcription factor called MarA that modulates efflux pump and porin expression. In this work, we show that MarA is also a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon. The operon, known to influence the formation of curli fibres and colanic acid, is usually expressed during periods of starvation. Hence, the ycgZ-ymgABC promoter is recognised by σ38 (RpoS)-associated RNA polymerase (RNAP). Surprisingly, MarA does not influence σ38 -dependent transcription. Instead, MarA drives transcription by the housekeeping σ70 -associated RNAP. The effects of MarA on ycgZ-ymgABC expression are coupled with biofilm formation by the rcsCDB phosphorelay system, with YcgZ, YmgA and YmgB forming a complex that directly interacts with the histidine kinase domain of RcsC.

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KW - Multienzyme Complexes/genetics

KW - Phosphoprotein Phosphatases/genetics

KW - Porins/genetics

KW - Protein Kinases/genetics

KW - Sigma Factor/genetics

KW - Transcription, Genetic/genetics

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