The chemical journey of Europium(III) through winter rye (Secale cereale L.): Understanding through mass spectrometry and chemical microscopy

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Julia Stadler
  • Manja Vogel
  • Robin Steudtner
  • Björn Drobot
  • Anna L. Kogiomtzidis
  • Martin Weiss
  • Clemens Walther

Externe Organisationen

  • Helmholtz-Zentrum Dresden-Rossendorf (HZDR)
  • VKTA – Strahlenschutz, Analytik & Entsorgung Rossendorf e.V (VKTA)
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Details

OriginalspracheEnglisch
Aufsatznummer137252
Seitenumfang12
FachzeitschriftCHEMOSPHERE
Jahrgang313
Frühes Online-Datum17 Nov. 2022
PublikationsstatusVeröffentlicht - Feb. 2023

Abstract

A combination of biochemical preparation methods with microscopic, spectroscopic, and mass spectrometric analysis techniques as contemplating state of the art application, was used for direct visualization, localization, and chemical identification of europium in plants. This works illustrates the chemical journey of europium (Eu(III)) through winter rye (Secale cereale L.), providing insight into the possibilities of speciation for Rare Earth Elements (REE) and trivalent f-elements. Kinetic experiments of contaminated plants show a maximum europium concentration in Secale cereale L. after four days. Transport of the element through the vascular bundle was confirmed with Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDS). For chemical speciation, plants were grown in a liquid nutrition medium, whereby Eu(III) species distribution could be measured by mass spectrometry and luminescence measurements. Both techniques confirm the occurrence of Eu malate species in the nutrition medium, and further analysis of the plant was performed. Luminescence results indicate a change in Eu(III) species distribution from root tip to plant leaves. Microscopic analysis show at least three different Eu(III) species with potential binding to organic and inorganic phosphate groups and a Eu(III) protein complex. With plant root extraction, further europium species could be identified by using Electrospray Ionization Mass Spectrometry (ESI MS). Complexation with malate, citrate, a combined malate-citrate ligand, and aspartate was confirmed mostly in a 1:1 stoichiometry (Eu:ligand). The combination of the used analytical techniques opens new possibilities in direct species analysis, especially regarding to the understanding of rare earth elements (REE) uptake in plants. This work provides a contribution in better understanding of plant mechanisms of the f-elements and their species uptake.

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The chemical journey of Europium(III) through winter rye (Secale cereale L.): Understanding through mass spectrometry and chemical microscopy. / Stadler, Julia; Vogel, Manja; Steudtner, Robin et al.
in: CHEMOSPHERE, Jahrgang 313, 137252, 02.2023.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Stadler J, Vogel M, Steudtner R, Drobot B, Kogiomtzidis AL, Weiss M et al. The chemical journey of Europium(III) through winter rye (Secale cereale L.): Understanding through mass spectrometry and chemical microscopy. CHEMOSPHERE. 2023 Feb;313:137252. Epub 2022 Nov 17. doi: 10.1016/j.chemosphere.2022.137252
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title = "The chemical journey of Europium(III) through winter rye (Secale cereale L.): Understanding through mass spectrometry and chemical microscopy",
abstract = "A combination of biochemical preparation methods with microscopic, spectroscopic, and mass spectrometric analysis techniques as contemplating state of the art application, was used for direct visualization, localization, and chemical identification of europium in plants. This works illustrates the chemical journey of europium (Eu(III)) through winter rye (Secale cereale L.), providing insight into the possibilities of speciation for Rare Earth Elements (REE) and trivalent f-elements. Kinetic experiments of contaminated plants show a maximum europium concentration in Secale cereale L. after four days. Transport of the element through the vascular bundle was confirmed with Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDS). For chemical speciation, plants were grown in a liquid nutrition medium, whereby Eu(III) species distribution could be measured by mass spectrometry and luminescence measurements. Both techniques confirm the occurrence of Eu malate species in the nutrition medium, and further analysis of the plant was performed. Luminescence results indicate a change in Eu(III) species distribution from root tip to plant leaves. Microscopic analysis show at least three different Eu(III) species with potential binding to organic and inorganic phosphate groups and a Eu(III) protein complex. With plant root extraction, further europium species could be identified by using Electrospray Ionization Mass Spectrometry (ESI MS). Complexation with malate, citrate, a combined malate-citrate ligand, and aspartate was confirmed mostly in a 1:1 stoichiometry (Eu:ligand). The combination of the used analytical techniques opens new possibilities in direct species analysis, especially regarding to the understanding of rare earth elements (REE) uptake in plants. This work provides a contribution in better understanding of plant mechanisms of the f-elements and their species uptake.",
keywords = "Europium, Imaging, Plant uptake, Rare earth elements, Species analysis",
author = "Julia Stadler and Manja Vogel and Robin Steudtner and Bj{\"o}rn Drobot and Kogiomtzidis, {Anna L.} and Martin Weiss and Clemens Walther",
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TY - JOUR

T1 - The chemical journey of Europium(III) through winter rye (Secale cereale L.)

T2 - Understanding through mass spectrometry and chemical microscopy

AU - Stadler, Julia

AU - Vogel, Manja

AU - Steudtner, Robin

AU - Drobot, Björn

AU - Kogiomtzidis, Anna L.

AU - Weiss, Martin

AU - Walther, Clemens

N1 - Funding Information: We would like to thank the Siebold-Sasse-Foundation for financial support.

PY - 2023/2

Y1 - 2023/2

N2 - A combination of biochemical preparation methods with microscopic, spectroscopic, and mass spectrometric analysis techniques as contemplating state of the art application, was used for direct visualization, localization, and chemical identification of europium in plants. This works illustrates the chemical journey of europium (Eu(III)) through winter rye (Secale cereale L.), providing insight into the possibilities of speciation for Rare Earth Elements (REE) and trivalent f-elements. Kinetic experiments of contaminated plants show a maximum europium concentration in Secale cereale L. after four days. Transport of the element through the vascular bundle was confirmed with Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDS). For chemical speciation, plants were grown in a liquid nutrition medium, whereby Eu(III) species distribution could be measured by mass spectrometry and luminescence measurements. Both techniques confirm the occurrence of Eu malate species in the nutrition medium, and further analysis of the plant was performed. Luminescence results indicate a change in Eu(III) species distribution from root tip to plant leaves. Microscopic analysis show at least three different Eu(III) species with potential binding to organic and inorganic phosphate groups and a Eu(III) protein complex. With plant root extraction, further europium species could be identified by using Electrospray Ionization Mass Spectrometry (ESI MS). Complexation with malate, citrate, a combined malate-citrate ligand, and aspartate was confirmed mostly in a 1:1 stoichiometry (Eu:ligand). The combination of the used analytical techniques opens new possibilities in direct species analysis, especially regarding to the understanding of rare earth elements (REE) uptake in plants. This work provides a contribution in better understanding of plant mechanisms of the f-elements and their species uptake.

AB - A combination of biochemical preparation methods with microscopic, spectroscopic, and mass spectrometric analysis techniques as contemplating state of the art application, was used for direct visualization, localization, and chemical identification of europium in plants. This works illustrates the chemical journey of europium (Eu(III)) through winter rye (Secale cereale L.), providing insight into the possibilities of speciation for Rare Earth Elements (REE) and trivalent f-elements. Kinetic experiments of contaminated plants show a maximum europium concentration in Secale cereale L. after four days. Transport of the element through the vascular bundle was confirmed with Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDS). For chemical speciation, plants were grown in a liquid nutrition medium, whereby Eu(III) species distribution could be measured by mass spectrometry and luminescence measurements. Both techniques confirm the occurrence of Eu malate species in the nutrition medium, and further analysis of the plant was performed. Luminescence results indicate a change in Eu(III) species distribution from root tip to plant leaves. Microscopic analysis show at least three different Eu(III) species with potential binding to organic and inorganic phosphate groups and a Eu(III) protein complex. With plant root extraction, further europium species could be identified by using Electrospray Ionization Mass Spectrometry (ESI MS). Complexation with malate, citrate, a combined malate-citrate ligand, and aspartate was confirmed mostly in a 1:1 stoichiometry (Eu:ligand). The combination of the used analytical techniques opens new possibilities in direct species analysis, especially regarding to the understanding of rare earth elements (REE) uptake in plants. This work provides a contribution in better understanding of plant mechanisms of the f-elements and their species uptake.

KW - Europium

KW - Imaging

KW - Plant uptake

KW - Rare earth elements

KW - Species analysis

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U2 - 10.1016/j.chemosphere.2022.137252

DO - 10.1016/j.chemosphere.2022.137252

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VL - 313

JO - CHEMOSPHERE

JF - CHEMOSPHERE

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