The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria

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Externe Organisationen

  • Max-Planck-Institut für experimentelle Endokrinologie
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Details

OriginalspracheEnglisch
Seiten (von - bis)326-333
Seitenumfang8
FachzeitschriftPlanta
Jahrgang201
Ausgabenummer3
PublikationsstatusVeröffentlicht - März 1997

Abstract

The 23-kDa nuclear-encoded heat-shock protein (HSP) of Chenopodium rubrum L. is regulated by light at tile posttranslational level. Higher light intensities are more effective in inducing the accumulation of the mature protein under heat-shock conditions. Based on this and other properties the protein was considered to belong to the group of small chloroplastic HSPs. However, we have now obtained the following evidence that this 23-kDa HSP is localized in the mitochondria: (i) Immunogold-labelled protein was almost exclusively restricted to the mitochondria in electron microscope thin sections. (ii) rising purified, isolated mitochondria from potato tubers the in-vitro-synthesized translation product of 31 kDa was readily transported into mitochondria where it was processed to the 23-kDa product. (iii) The protein could be detected by Western blotting in a preparation of washed mitochondria of Chenopodium, while under the same conditions no signal could be obtained in a preparation of isolated chloroplasts. (iv) Finally, sequence comparison with the published sequences of mitochondrial proteins by Lenne et al. (1995, Biochem J 311:805 813)and LaFayette et al. (1996, Plant Mol Biol 31):159 169) showed clearly that the 23-kDa protein is considerably more similar to these two proteins than to the group of plastid small HSPs. From these data we infer that mitochondria are involved in the response of the plants to high light stress under heat-shock conditions.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik
  • Agrar- und Biowissenschaften (insg.)
  • Pflanzenkunde

Zitieren

The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria. / Debel, Karsten; Sierralta, Walter D.; Braun, Hans-Peter et al.
in: Planta, Jahrgang 201, Nr. 3, 03.1997, S. 326-333.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Debel K, Sierralta WD, Braun HP, Schmitz U, Kloppstech K. The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria. Planta. 1997 Mär;201(3):326-333. doi: 10.1007/s004250050074
Debel, Karsten ; Sierralta, Walter D. ; Braun, Hans-Peter et al. / The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria. in: Planta. 1997 ; Jahrgang 201, Nr. 3. S. 326-333.
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title = "The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria",
abstract = "The 23-kDa nuclear-encoded heat-shock protein (HSP) of Chenopodium rubrum L. is regulated by light at tile posttranslational level. Higher light intensities are more effective in inducing the accumulation of the mature protein under heat-shock conditions. Based on this and other properties the protein was considered to belong to the group of small chloroplastic HSPs. However, we have now obtained the following evidence that this 23-kDa HSP is localized in the mitochondria: (i) Immunogold-labelled protein was almost exclusively restricted to the mitochondria in electron microscope thin sections. (ii) rising purified, isolated mitochondria from potato tubers the in-vitro-synthesized translation product of 31 kDa was readily transported into mitochondria where it was processed to the 23-kDa product. (iii) The protein could be detected by Western blotting in a preparation of washed mitochondria of Chenopodium, while under the same conditions no signal could be obtained in a preparation of isolated chloroplasts. (iv) Finally, sequence comparison with the published sequences of mitochondrial proteins by Lenne et al. (1995, Biochem J 311:805 813)and LaFayette et al. (1996, Plant Mol Biol 31):159 169) showed clearly that the 23-kDa protein is considerably more similar to these two proteins than to the group of plastid small HSPs. From these data we infer that mitochondria are involved in the response of the plants to high light stress under heat-shock conditions.",
keywords = "Cell culture (plant), Chenopodium Heat-shock protein (mitochondria), Mitochondrion Protein transport (in vitro)",
author = "Karsten Debel and Sierralta, {Walter D.} and Hans-Peter Braun and Udo Schmitz and Klaus Kloppstech",
note = "Funding information: We gratefully acknowledge the skilful and excellent technical help by Mrs. Ingrid Bonig during the experiments. This work was generously supported by the Deutsche Forschungsgemeinschaft, Bonn, Germany. It was also carried out as part of a collaboration between Dr. S. Gepstein, Haifa Israel, and K.K. with the help of a grant (Stiftung Volkswagenwerk, Hannover) which was provided by the Ministry of Science and Arts of the Government of Niedersachsen, Germany.",
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pages = "326--333",
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TY - JOUR

T1 - The 23-kDa light-stress-regulated heat-shock protein of Chenopodium rubrum L. is located in the mitochondria

AU - Debel, Karsten

AU - Sierralta, Walter D.

AU - Braun, Hans-Peter

AU - Schmitz, Udo

AU - Kloppstech, Klaus

N1 - Funding information: We gratefully acknowledge the skilful and excellent technical help by Mrs. Ingrid Bonig during the experiments. This work was generously supported by the Deutsche Forschungsgemeinschaft, Bonn, Germany. It was also carried out as part of a collaboration between Dr. S. Gepstein, Haifa Israel, and K.K. with the help of a grant (Stiftung Volkswagenwerk, Hannover) which was provided by the Ministry of Science and Arts of the Government of Niedersachsen, Germany.

PY - 1997/3

Y1 - 1997/3

N2 - The 23-kDa nuclear-encoded heat-shock protein (HSP) of Chenopodium rubrum L. is regulated by light at tile posttranslational level. Higher light intensities are more effective in inducing the accumulation of the mature protein under heat-shock conditions. Based on this and other properties the protein was considered to belong to the group of small chloroplastic HSPs. However, we have now obtained the following evidence that this 23-kDa HSP is localized in the mitochondria: (i) Immunogold-labelled protein was almost exclusively restricted to the mitochondria in electron microscope thin sections. (ii) rising purified, isolated mitochondria from potato tubers the in-vitro-synthesized translation product of 31 kDa was readily transported into mitochondria where it was processed to the 23-kDa product. (iii) The protein could be detected by Western blotting in a preparation of washed mitochondria of Chenopodium, while under the same conditions no signal could be obtained in a preparation of isolated chloroplasts. (iv) Finally, sequence comparison with the published sequences of mitochondrial proteins by Lenne et al. (1995, Biochem J 311:805 813)and LaFayette et al. (1996, Plant Mol Biol 31):159 169) showed clearly that the 23-kDa protein is considerably more similar to these two proteins than to the group of plastid small HSPs. From these data we infer that mitochondria are involved in the response of the plants to high light stress under heat-shock conditions.

AB - The 23-kDa nuclear-encoded heat-shock protein (HSP) of Chenopodium rubrum L. is regulated by light at tile posttranslational level. Higher light intensities are more effective in inducing the accumulation of the mature protein under heat-shock conditions. Based on this and other properties the protein was considered to belong to the group of small chloroplastic HSPs. However, we have now obtained the following evidence that this 23-kDa HSP is localized in the mitochondria: (i) Immunogold-labelled protein was almost exclusively restricted to the mitochondria in electron microscope thin sections. (ii) rising purified, isolated mitochondria from potato tubers the in-vitro-synthesized translation product of 31 kDa was readily transported into mitochondria where it was processed to the 23-kDa product. (iii) The protein could be detected by Western blotting in a preparation of washed mitochondria of Chenopodium, while under the same conditions no signal could be obtained in a preparation of isolated chloroplasts. (iv) Finally, sequence comparison with the published sequences of mitochondrial proteins by Lenne et al. (1995, Biochem J 311:805 813)and LaFayette et al. (1996, Plant Mol Biol 31):159 169) showed clearly that the 23-kDa protein is considerably more similar to these two proteins than to the group of plastid small HSPs. From these data we infer that mitochondria are involved in the response of the plants to high light stress under heat-shock conditions.

KW - Cell culture (plant)

KW - Chenopodium Heat-shock protein (mitochondria)

KW - Mitochondrion Protein transport (in vitro)

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U2 - 10.1007/s004250050074

DO - 10.1007/s004250050074

M3 - Article

C2 - 9129338

AN - SCOPUS:1842292085

VL - 201

SP - 326

EP - 333

JO - Planta

JF - Planta

SN - 0032-0935

IS - 3

ER -

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