Synchronizing droplet microfluidics and ion mobility spectrometry via the electrospray current recorded with a tailored low-noise, high-gain current amplifier

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • T. Ostermeier
  • A. Nitschke
  • C. Thoben
  • M. Hitzemann
  • K. Welters
  • D. Belder
  • S. Zimmermann

Externe Organisationen

  • Universität Leipzig
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Details

OriginalspracheEnglisch
Aufsatznummer129040
FachzeitschriftTALANTA
Jahrgang298
Frühes Online-Datum28 Okt. 2025
PublikationsstatusVeröffentlicht - 1 Feb. 2026

Abstract

In the process of coupling ion mobility spectrometry (IMS) and droplet microfluidics, the sample to be analyzed is ionized by electrospray ionization (ESI), thereby transferring the liquid samples into the gas phase with concurrent ionization. In contradistinction to conventional ESI, in droplet microfluidics the sample to be analyzed is present in individual droplets, which are separated from each other by an oil phase resulting in the process of ESI becoming discontinuous due to alternating sample droplets and oil phase. Typically, averaging is necessary in IMS to suppress noise and thus improve detection limits. However, in combination with droplet microfluidics, continuous averaging leads to reduced signal-to-noise ratio, because the spectra of the oil phase not containing any information are included into averaging. To avoid this effect, it is imperative to synchronize the ESI-IMS with the droplet microfluidics using a suited trigger signal. Therefore, we present a fast, low-noise, high-gain current amplifier that can operate at high electrical potential and allows for monitoring the ESI current in both polarities. The design of this amplifier is predicated on the current change of the ESI current during the volume transition between oil and droplet inside the emitter. This transition has been mathematically modelled and incorporated as a volume-dependent term within an existing equation for the ESI current. Finally, we present experimental data to support the idea of increasing signal-to-noise ratio by triggering the ESI-IMS via the electrospray current. Furthermore, triggering the IMS facilitates droplet-wise storage of data.

ASJC Scopus Sachgebiete

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Synchronizing droplet microfluidics and ion mobility spectrometry via the electrospray current recorded with a tailored low-noise, high-gain current amplifier. / Ostermeier, T.; Nitschke, A.; Thoben, C. et al.
in: TALANTA, Jahrgang 298, 129040, 01.02.2026.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Ostermeier T, Nitschke A, Thoben C, Hitzemann M, Welters K, Belder D et al. Synchronizing droplet microfluidics and ion mobility spectrometry via the electrospray current recorded with a tailored low-noise, high-gain current amplifier. TALANTA. 2026 Feb 1;298:129040. Epub 2025 Okt 28. doi: 10.1016/j.talanta.2025.129040
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abstract = "In the process of coupling ion mobility spectrometry (IMS) and droplet microfluidics, the sample to be analyzed is ionized by electrospray ionization (ESI), thereby transferring the liquid samples into the gas phase with concurrent ionization. In contradistinction to conventional ESI, in droplet microfluidics the sample to be analyzed is present in individual droplets, which are separated from each other by an oil phase resulting in the process of ESI becoming discontinuous due to alternating sample droplets and oil phase. Typically, averaging is necessary in IMS to suppress noise and thus improve detection limits. However, in combination with droplet microfluidics, continuous averaging leads to reduced signal-to-noise ratio, because the spectra of the oil phase not containing any information are included into averaging. To avoid this effect, it is imperative to synchronize the ESI-IMS with the droplet microfluidics using a suited trigger signal. Therefore, we present a fast, low-noise, high-gain current amplifier that can operate at high electrical potential and allows for monitoring the ESI current in both polarities. The design of this amplifier is predicated on the current change of the ESI current during the volume transition between oil and droplet inside the emitter. This transition has been mathematically modelled and incorporated as a volume-dependent term within an existing equation for the ESI current. Finally, we present experimental data to support the idea of increasing signal-to-noise ratio by triggering the ESI-IMS via the electrospray current. Furthermore, triggering the IMS facilitates droplet-wise storage of data.",
keywords = "Droplet microfluidics, Electrospray ionization, ESI, ESI current equation, Ion mobility spectrometry, Volume transition",
author = "T. Ostermeier and A. Nitschke and C. Thoben and M. Hitzemann and K. Welters and D. Belder and S. Zimmermann",
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AU - Ostermeier, T.

AU - Nitschke, A.

AU - Thoben, C.

AU - Hitzemann, M.

AU - Welters, K.

AU - Belder, D.

AU - Zimmermann, S.

N1 - Publisher Copyright: © 2025 The Authors.

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Y1 - 2026/2/1

N2 - In the process of coupling ion mobility spectrometry (IMS) and droplet microfluidics, the sample to be analyzed is ionized by electrospray ionization (ESI), thereby transferring the liquid samples into the gas phase with concurrent ionization. In contradistinction to conventional ESI, in droplet microfluidics the sample to be analyzed is present in individual droplets, which are separated from each other by an oil phase resulting in the process of ESI becoming discontinuous due to alternating sample droplets and oil phase. Typically, averaging is necessary in IMS to suppress noise and thus improve detection limits. However, in combination with droplet microfluidics, continuous averaging leads to reduced signal-to-noise ratio, because the spectra of the oil phase not containing any information are included into averaging. To avoid this effect, it is imperative to synchronize the ESI-IMS with the droplet microfluidics using a suited trigger signal. Therefore, we present a fast, low-noise, high-gain current amplifier that can operate at high electrical potential and allows for monitoring the ESI current in both polarities. The design of this amplifier is predicated on the current change of the ESI current during the volume transition between oil and droplet inside the emitter. This transition has been mathematically modelled and incorporated as a volume-dependent term within an existing equation for the ESI current. Finally, we present experimental data to support the idea of increasing signal-to-noise ratio by triggering the ESI-IMS via the electrospray current. Furthermore, triggering the IMS facilitates droplet-wise storage of data.

AB - In the process of coupling ion mobility spectrometry (IMS) and droplet microfluidics, the sample to be analyzed is ionized by electrospray ionization (ESI), thereby transferring the liquid samples into the gas phase with concurrent ionization. In contradistinction to conventional ESI, in droplet microfluidics the sample to be analyzed is present in individual droplets, which are separated from each other by an oil phase resulting in the process of ESI becoming discontinuous due to alternating sample droplets and oil phase. Typically, averaging is necessary in IMS to suppress noise and thus improve detection limits. However, in combination with droplet microfluidics, continuous averaging leads to reduced signal-to-noise ratio, because the spectra of the oil phase not containing any information are included into averaging. To avoid this effect, it is imperative to synchronize the ESI-IMS with the droplet microfluidics using a suited trigger signal. Therefore, we present a fast, low-noise, high-gain current amplifier that can operate at high electrical potential and allows for monitoring the ESI current in both polarities. The design of this amplifier is predicated on the current change of the ESI current during the volume transition between oil and droplet inside the emitter. This transition has been mathematically modelled and incorporated as a volume-dependent term within an existing equation for the ESI current. Finally, we present experimental data to support the idea of increasing signal-to-noise ratio by triggering the ESI-IMS via the electrospray current. Furthermore, triggering the IMS facilitates droplet-wise storage of data.

KW - Droplet microfluidics

KW - Electrospray ionization

KW - ESI

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