Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 358-361 |
| Seitenumfang | 4 |
| Fachzeitschrift | Biotechnology and bioengineering |
| Jahrgang | 70 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 12 Sept. 2000 |
| Extern publiziert | Ja |
Abstract
In high-cell density cultures of Escherichia coli, the amount of ammonia required to maintain a constant pH was an effective on-line measure of biomass. When acetic acid formation occurred, biomass estimates were corrected by additional measurements of the culture conductivity. In the absence of acetic acid excretion, the culture conductivity decreased approximately linearly during the fed-batch phase of the high-cell density cultivation. Upward deviation of the culture conductivity from this trend was proportional to the acetate concentration in the medium. Thus, simultaneous determination of the acetic acid concentration and correction of biomass estimates from ammonia consumption were possible by conductivity measurements. (C) 2000 John Wiley and Sons, Inc.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Biotechnology and bioengineering, Jahrgang 70, Nr. 3, 12.09.2000, S. 358-361.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Simple technique for simultaneous on-line estimation of biomass and acetate from base consumption and conductivity measurements in high-cell density cultures of Escherichia coli
AU - Hoffmann, Frank
AU - Schmidt, Michael
AU - Rinas, Ursula
PY - 2000/9/12
Y1 - 2000/9/12
N2 - In high-cell density cultures of Escherichia coli, the amount of ammonia required to maintain a constant pH was an effective on-line measure of biomass. When acetic acid formation occurred, biomass estimates were corrected by additional measurements of the culture conductivity. In the absence of acetic acid excretion, the culture conductivity decreased approximately linearly during the fed-batch phase of the high-cell density cultivation. Upward deviation of the culture conductivity from this trend was proportional to the acetate concentration in the medium. Thus, simultaneous determination of the acetic acid concentration and correction of biomass estimates from ammonia consumption were possible by conductivity measurements. (C) 2000 John Wiley and Sons, Inc.
AB - In high-cell density cultures of Escherichia coli, the amount of ammonia required to maintain a constant pH was an effective on-line measure of biomass. When acetic acid formation occurred, biomass estimates were corrected by additional measurements of the culture conductivity. In the absence of acetic acid excretion, the culture conductivity decreased approximately linearly during the fed-batch phase of the high-cell density cultivation. Upward deviation of the culture conductivity from this trend was proportional to the acetate concentration in the medium. Thus, simultaneous determination of the acetic acid concentration and correction of biomass estimates from ammonia consumption were possible by conductivity measurements. (C) 2000 John Wiley and Sons, Inc.
KW - Acetate
KW - Biomass
KW - Estimation
KW - On-line
UR - http://www.scopus.com/inward/record.url?scp=84975842426&partnerID=8YFLogxK
U2 - 10.1002/1097-0290(20001105)70:3<358::AID-BIT14>3.0.CO;2-T
DO - 10.1002/1097-0290(20001105)70:3<358::AID-BIT14>3.0.CO;2-T
M3 - Article
C2 - 10992241
AN - SCOPUS:84975842426
VL - 70
SP - 358
EP - 361
JO - Biotechnology and bioengineering
JF - Biotechnology and bioengineering
SN - 0006-3592
IS - 3
ER -