Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 739-783 |
| Seitenumfang | 45 |
| Fachzeitschrift | Biotechnology advances |
| Jahrgang | 36 |
| Ausgabenummer | 3 |
| Frühes Online-Datum | 5 Feb. 2018 |
| Publikationsstatus | Veröffentlicht - Mai 2018 |
Abstract
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
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in: Biotechnology advances, Jahrgang 36, Nr. 3, 05.2018, S. 739-783.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung
}
TY - JOUR
T1 - Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.
AU - He, Yi
AU - Wang, Bin
AU - Chen, Wanping
AU - Cox, Russell J.
AU - He, Jingren
AU - Chen, Fusheng
N1 - © 2018 Elsevier Inc. All rights reserved.
PY - 2018/5
Y1 - 2018/5
N2 - High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
AB - High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
KW - Aspergillus spp.
KW - Combinatorial biosynthesis
KW - DNA assembly
KW - Heterologous expression
KW - Microbial secondary metabolites
KW - Pathway engineering
KW - Promoter Regions, Genetic
KW - Multigene Family
KW - Bridged Bicyclo Compounds, Heterocyclic
KW - Cloning, Molecular/methods
KW - Computational Biology/methods
KW - Microorganisms, Genetically-Modified
KW - Tricarboxylic Acids
KW - Lovastatin/genetics
KW - Polyketides/metabolism
KW - Polymerase Chain Reaction/methods
KW - Recombination, Genetic
KW - Genetic Engineering/methods
KW - Secondary Metabolism/genetics
KW - Biosynthetic Pathways/genetics
KW - Aspergillus/genetics
UR - http://www.scopus.com/inward/record.url?scp=85042365902&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2018.02.001
DO - 10.1016/j.biotechadv.2018.02.001
M3 - Article
C2 - 29421302
AN - SCOPUS:85042365902
VL - 36
SP - 739
EP - 783
JO - Biotechnology advances
JF - Biotechnology advances
SN - 0734-9750
IS - 3
ER -