Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

Autoren

  • Hannes Kamin
  • Lena Nolte
  • Jochen Maurer
  • Andreas Bleilevens
  • Elmar Stickeler
  • Sonja Johannsmeier
  • Dag Heinemann
  • Tammo Ripken

Externe Organisationen

  • Laser Zentrum Hannover e.V. (LZH)
  • Rheinisch-Westfälische Technische Hochschule Aachen (RWTH)
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Details

OriginalspracheEnglisch
Titel des SammelwerksThree-Dimensional and Multidimensional Microscopy
UntertitelImage Acquisition and Processing XXIX
Herausgeber/-innenThomas G. Brown, Tony Wilson, Laura Waller
Herausgeber (Verlag)SPIE
ISBN (elektronisch)9781510648036
PublikationsstatusVeröffentlicht - 2 März 2022
VeranstaltungThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022 - Virtual, Online
Dauer: 20 Feb. 202224 Feb. 2022

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band11966
ISSN (Print)1605-7422

Abstract

The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.

ASJC Scopus Sachgebiete

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Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography. / Kamin, Hannes; Nolte, Lena; Maurer, Jochen et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX. Hrsg. / Thomas G. Brown; Tony Wilson; Laura Waller. SPIE, 2022. 119660B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 11966).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

Kamin, H, Nolte, L, Maurer, J, Bleilevens, A, Stickeler, E, Johannsmeier, S, Heinemann, D & Ripken, T 2022, Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography. in TG Brown, T Wilson & L Waller (Hrsg.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX., 119660B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Bd. 11966, SPIE, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022, Virtual, Online, 20 Feb. 2022. https://doi.org/10.1117/12.2609425
Kamin, H., Nolte, L., Maurer, J., Bleilevens, A., Stickeler, E., Johannsmeier, S., Heinemann, D., & Ripken, T. (2022). Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography. In T. G. Brown, T. Wilson, & L. Waller (Hrsg.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX Artikel 119660B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 11966). SPIE. https://doi.org/10.1117/12.2609425
Kamin H, Nolte L, Maurer J, Bleilevens A, Stickeler E, Johannsmeier S et al. Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography. in Brown TG, Wilson T, Waller L, Hrsg., Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX. SPIE. 2022. 119660B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2609425
Kamin, Hannes ; Nolte, Lena ; Maurer, Jochen et al. / Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX. Hrsg. / Thomas G. Brown ; Tony Wilson ; Laura Waller. SPIE, 2022. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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title = "Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography",
abstract = "The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.",
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AU - Kamin, Hannes

AU - Nolte, Lena

AU - Maurer, Jochen

AU - Bleilevens, Andreas

AU - Stickeler, Elmar

AU - Johannsmeier, Sonja

AU - Heinemann, Dag

AU - Ripken, Tammo

N1 - Funding Information: This work is funded by the Federal Ministry of Education and Research, Germany, Grant no. 031L0146C

PY - 2022/3/2

Y1 - 2022/3/2

N2 - The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.

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KW - 3D imaging

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A2 - Wilson, Tony

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Y2 - 20 February 2022 through 24 February 2022

ER -

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