TY - JOUR

T1 - Proteomic analysis of two divergently responding potato genotypes (Solanum tuberosum L.) following osmotic stress treatment in vitro

AU - Bündig, C.

AU - Jozefowicz, A.M.

AU - Mock, H.-P.

AU - Winkelmann, T.

N1 - Funding Information: The study was conducted with funding provided by the Federal Ministry of Food and Agriculture (BMEL) through the Agency of Renewable Resources (FNR), funding nos. 22023511 (LUH) and 22023411 (IPK). The authors would like to thank Dagmar Lewejohann, Dr. Christin Lorenz and Annegret Wolf for their support and Prof. Dr. Hans-Peter Braun for partial use of his proteomic laboratory.

PY - 2016/6/30

Y1 - 2016/6/30

N2 - Starch potatoes (Solanum tuberosum L.) are of interest for production of starch, ethanol, and biopolymers. Due to the predicted increase in drought periods, the breeding of starch potatoes for drought tolerance is essential. This study aims to elucidate the physiological mechanisms that give rise to drought tolerance. Two genotypes contrasting in drought tolerance were compared. We applied osmotic stress which is a known component of drought stress under in vitro conditions. Shoot tips were harvested after 11 days of culture on control medium and medium supplied with 0.2 M sorbitol. Their proteomes were analyzed using two-dimensional isoelectric focussing sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D-IEF/SDS-PAGE). Of a total of 679 distinct protein spots, 118 and 20 spots with differential abundance were found in the sensitive and the tolerant genotype, respectively, after the application of stress. Using mass spectrometry, the proteins in 100 differentially abundant spots were identified; a majority of these proteins were from the chloroplast. For the sensitive genotype, an increase in the abundance of proteinase inhibitors and their precursors, changes in stress responsive proteins and an altered RNA/DNA-binding response were observed. The differentially abundant spots of the tolerant genotype comprised one chaperone and one hydrogen peroxide detoxifying protein. Biological significance Our findings reveal that the two genotypes have different responses to osmotic stress in terms of protein degradation and reactive oxygen species (ROS) scavenging and production. Our data suggest that the tolerant genotype might adjust to the applied stress more quickly. A comparative temporal analysis might provide further insights into these rapid changes and assist in the development of biomarkers.

AB - Starch potatoes (Solanum tuberosum L.) are of interest for production of starch, ethanol, and biopolymers. Due to the predicted increase in drought periods, the breeding of starch potatoes for drought tolerance is essential. This study aims to elucidate the physiological mechanisms that give rise to drought tolerance. Two genotypes contrasting in drought tolerance were compared. We applied osmotic stress which is a known component of drought stress under in vitro conditions. Shoot tips were harvested after 11 days of culture on control medium and medium supplied with 0.2 M sorbitol. Their proteomes were analyzed using two-dimensional isoelectric focussing sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D-IEF/SDS-PAGE). Of a total of 679 distinct protein spots, 118 and 20 spots with differential abundance were found in the sensitive and the tolerant genotype, respectively, after the application of stress. Using mass spectrometry, the proteins in 100 differentially abundant spots were identified; a majority of these proteins were from the chloroplast. For the sensitive genotype, an increase in the abundance of proteinase inhibitors and their precursors, changes in stress responsive proteins and an altered RNA/DNA-binding response were observed. The differentially abundant spots of the tolerant genotype comprised one chaperone and one hydrogen peroxide detoxifying protein. Biological significance Our findings reveal that the two genotypes have different responses to osmotic stress in terms of protein degradation and reactive oxygen species (ROS) scavenging and production. Our data suggest that the tolerant genotype might adjust to the applied stress more quickly. A comparative temporal analysis might provide further insights into these rapid changes and assist in the development of biomarkers.

KW - In vitro

KW - Mass spectrometry

KW - Osmotic stress

KW - Proteomics

KW - Solanum tuberosum

KW - Stress response

UR - http://www.scopus.com/inward/record.url?scp=84969531850&partnerID=8YFLogxK

U2 - 10.1016/j.jprot.2016.04.048

DO - 10.1016/j.jprot.2016.04.048

M3 - Article

VL - 143

SP - 227

EP - 241

JO - Journal of proteomics

JF - Journal of proteomics

SN - 1874-3919

ER -