Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 112-123 |
Seitenumfang | 12 |
Fachzeitschrift | Reproduction, Fertility and Development |
Jahrgang | 36 |
Ausgabenummer | 2 |
Publikationsstatus | Veröffentlicht - 22 Sept. 2023 |
Abstract
Context. Genome editing enables the introduction of beneficial sequence variants into the genomes of animals with high genetic merit in a single generation. This can be achieved by introducing variants into primary cells followed by producing a live animal from these cells by somatic cell nuclear transfer cloning. The latter step is associated with low efficiencies and developmental problems due to incorrect reprogramming of the donor cells, causing animal welfare concerns. Direct editing of fertilised one-cell embryos could circumvent this issue and might better integrate with genetic improvement strategies implemented by the industry. Methods. In vitro fertilised zygotes were injected with TALEN editors and repair template to introduce a known coat colour dilution mutation in the PMEL gene. Embryo biopsies of injected embryos were screened by polymerase chain reaction and sequencing for intended biallelic edits before transferring verified embryos into recipients for development to term. Calves were genotyped and their coats scanned with visible and hyperspectral cameras to assess thermal energy absorption. Key results. Multiple non-mosaic calves with precision edited genotypes were produced, including calves from high genetic merit parents. Compared to controls, the edited calves showed a strong coat colour dilution which was associated with lower thermal energy absorbance. Conclusions. Although biopsy screening was not absolutely accurate, non-mosaic, precisely edited calves can be readily produced by embryo-mediated editing. The lighter coat colouring caused by the PMEL mutation can lower radiative heat gain which might help to reduce heat stress. Implications. The study validates putative causative sequence variants to rapidly adapt grazing cattle to changing environmental conditions.
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in: Reproduction, Fertility and Development, Jahrgang 36, Nr. 2, 22.09.2023, S. 112-123.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - Production of light-coloured, low heat-absorbing Holstein Friesian cattle by precise embryo-mediated genome editing
AU - Wei, Jingwei
AU - Brophy, Brigid
AU - Cole, Sally Ann
AU - Leath, Shane
AU - Oback, Björn
AU - Boch, Jens
AU - Wells, David N.
AU - Laible, Götz
N1 - Funding Information: The authors would like to thank members of the Animal Biotech team and farm staff for their support. We are grateful for funding of this study by AgResearch, CRV Ltd, Livestock Improvement Corporation and the Ministry of Business, Innovation and Employment.
PY - 2023/9/22
Y1 - 2023/9/22
N2 - Context. Genome editing enables the introduction of beneficial sequence variants into the genomes of animals with high genetic merit in a single generation. This can be achieved by introducing variants into primary cells followed by producing a live animal from these cells by somatic cell nuclear transfer cloning. The latter step is associated with low efficiencies and developmental problems due to incorrect reprogramming of the donor cells, causing animal welfare concerns. Direct editing of fertilised one-cell embryos could circumvent this issue and might better integrate with genetic improvement strategies implemented by the industry. Methods. In vitro fertilised zygotes were injected with TALEN editors and repair template to introduce a known coat colour dilution mutation in the PMEL gene. Embryo biopsies of injected embryos were screened by polymerase chain reaction and sequencing for intended biallelic edits before transferring verified embryos into recipients for development to term. Calves were genotyped and their coats scanned with visible and hyperspectral cameras to assess thermal energy absorption. Key results. Multiple non-mosaic calves with precision edited genotypes were produced, including calves from high genetic merit parents. Compared to controls, the edited calves showed a strong coat colour dilution which was associated with lower thermal energy absorbance. Conclusions. Although biopsy screening was not absolutely accurate, non-mosaic, precisely edited calves can be readily produced by embryo-mediated editing. The lighter coat colouring caused by the PMEL mutation can lower radiative heat gain which might help to reduce heat stress. Implications. The study validates putative causative sequence variants to rapidly adapt grazing cattle to changing environmental conditions.
AB - Context. Genome editing enables the introduction of beneficial sequence variants into the genomes of animals with high genetic merit in a single generation. This can be achieved by introducing variants into primary cells followed by producing a live animal from these cells by somatic cell nuclear transfer cloning. The latter step is associated with low efficiencies and developmental problems due to incorrect reprogramming of the donor cells, causing animal welfare concerns. Direct editing of fertilised one-cell embryos could circumvent this issue and might better integrate with genetic improvement strategies implemented by the industry. Methods. In vitro fertilised zygotes were injected with TALEN editors and repair template to introduce a known coat colour dilution mutation in the PMEL gene. Embryo biopsies of injected embryos were screened by polymerase chain reaction and sequencing for intended biallelic edits before transferring verified embryos into recipients for development to term. Calves were genotyped and their coats scanned with visible and hyperspectral cameras to assess thermal energy absorption. Key results. Multiple non-mosaic calves with precision edited genotypes were produced, including calves from high genetic merit parents. Compared to controls, the edited calves showed a strong coat colour dilution which was associated with lower thermal energy absorbance. Conclusions. Although biopsy screening was not absolutely accurate, non-mosaic, precisely edited calves can be readily produced by embryo-mediated editing. The lighter coat colouring caused by the PMEL mutation can lower radiative heat gain which might help to reduce heat stress. Implications. The study validates putative causative sequence variants to rapidly adapt grazing cattle to changing environmental conditions.
KW - cattle
KW - coat color dilution
KW - embryo-mediated
KW - genome editing
KW - heat stress
KW - homology-directed repair
KW - microinjection
KW - P. Leu18del
KW - PMEL
KW - pre-melanosome protein 17
KW - TALEN
UR - http://www.scopus.com/inward/record.url?scp=85179642582&partnerID=8YFLogxK
U2 - 10.1071/RD23163
DO - 10.1071/RD23163
M3 - Review article
C2 - 38064192
AN - SCOPUS:85179642582
VL - 36
SP - 112
EP - 123
JO - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
SN - 1031-3613
IS - 2
ER -